Protein

Protein accession
A0A0U4K7B3 [UniProt]
Representative
3X7ua
Source
UniProt (cluster: phalp2_20487)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTFSSLISGTIPHNNKFSSRQGVPVSRIIQHHFAGSSDERLRSATEQASSHYVIYNDGSIWGLVPEEYRAWTSGSFEADAPSITYEVQNSSFQINGNDDDPNSWAVSDAAYNSIVLLTADIARRYGFGAVTPTNYQGHRQWYSTACPGGFLWARMDNTRSLANKNLSGATVPTPTPSTPPVAGKTVWQLADEVLAGHHGSGDDRKISLGIQYDAVQAEINRRYGVVVVAPAEKTVSQLADEVLAGAHGNGEQRRASLGNRFDEVQNEINRRLGGGGVAPQGLNIAQLADAVMRGEYGSGQDRINRLGANYDAVQQEVNRRLGGNASPVANINALADAVLRGEYGNGDERVRRLGANYAAVQAEINRRYA
Physico‐chemical
properties
protein length:369 AA
molecular weight:39878,4 Da
isoelectric point:6,09
hydropathy:-0,48
Representative Protein Details
Accession
3X7ua
Protein name
3X7ua
Sequence length
326 AA
Molecular weight
34646,42760 Da
Isoelectric point
9,87238
Sequence
MTFSHLISGTVPHHNKLSSRAGSPIVRVIPHHHAATSNAGLDRLTNPNEQASANYIIMSSGRIYGQVPEEYRAWTSGSHEADAPSITFEIQNSGVAVNGNDNDPNSWPISSAAFHSTVALIADIAKRYQWGGVGTYNVRGHREFYSTACPGGYVWNQLGNIRASANALALGKVTNVTPVSKPAPAAPSIKGKTVWTLAQEVIKGVYGSGDARKKALGSQYAAVQAEVNRILAAPAAKLPSKGKKSTAQLVDEVLAGVHGSGDARKKSLGGRYDEVQAAVNRRVGGVNISALADAVIRGEYGTGAERQRRLGVNYVAVQAEVNRRYR
Other Proteins in cluster: phalp2_20487
Total (incl. this protein): 81 Avg length: 340,6 Avg pI: 8,38
Protein ID Length (AA) pI
3X7ua 326 9,87238
10GLI 253 9,59691
15Jg8 381 8,83425
1Fvh7 370 9,76556
1J2hk 327 9,45347
1KVSZ 374 9,28366
1LsAw 376 9,09599
1Ly93 271 8,45801
1MfDA 326 8,71756
1Rg00 345 9,98939
1hUmc 356 4,55874
24FWQ 312 8,93346
2G9P4 366 5,83608
3V3Hx 368 5,81266
3V40f 368 6,26482
3fQ6z 335 8,96654
3m20a 309 9,30068
40xai 363 4,60813
4FT5B 384 9,33375
4FWaj 380 9,46881
4XRWq 374 5,20005
4ejBu 325 9,19269
5BYmU 373 6,39100
5CiQt 373 6,22344
5PHV1 309 9,13422
5Tixj 309 9,01772
5XKBt 309 9,23369
5tDgs 310 9,01347
5zNz4 267 4,79445
68sak 338 8,72291
696ng 318 9,10482
6BRin 269 4,64496
6Dh5k 300 9,57879
6F68N 322 9,72855
6a8rX 309 9,13738
6siSe 330 9,15601
6uMYX 309 9,05628
7DXxF 369 9,62341
7s7Ot 312 9,01334
7uITa 310 8,84321
7yFMi 312 8,60345
7ydy8 310 8,99954
8KrC6 374 7,88063
8KrC7 369 6,08503
8KrC8 369 7,11172
8KrCg 317 4,75375
8KrCh 317 4,81537
8fxm2 324 9,99597
8mQRb 331 8,73793
K9vG 252 8,96493
ZoiK 253 9,97863
fnnl 321 9,56171
fsaG 357 4,82292
fssF 322 9,62444
ftjr 374 9,18044
fwJ2 368 7,92370
ynWa 252 8,57115
A0A218M5G8 374 7,88063
A0A222ZGI4 369 7,11172
A0A2Z5HF94 374 7,88044
A0A345KL46 374 7,88044
A0A345KUG3 374 7,88044
A0A5J6TE42 372 9,83467
A0A5J6THU6 377 9,23872
A0A5J6TI33 377 9,23872
A0A5J6TJS0 372 9,83467
A0A5J6TJU6 374 9,18070
A0A5J6TK72 377 9,23872
A0A5J6TPP9 377 9,23872
A0A5J6TU82 377 9,23872
A0A5J6TVH2 377 9,23872
A0A5P8D8P8 374 9,86929
A0A7T3KBZ2 374 9,86929
A0A9E7NIH9 374 7,88044
A0AAE7VHQ9 374 7,10882
A0A9E7J0G7 326 9,74693
A0A9E7QA88 326 9,74693
A0A9E7QI10 326 9,74693
A0AA96HKS9 326 9,74693
A0AA96NJ95 326 9,74693
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_1607
7ZyR0
 345 40,0% 327 4.272E-100
2 phalp2_10280
1cMFf
 75 31,0% 351 9.935E-62
3 phalp2_2230
4k5IM
 1 30,0% 293 1.152E-52
4 phalp2_15173
ovmV
 70 27,7% 306 2.387E-48
5 phalp2_30111
3bZzl
 4 43,7% 201 6.326E-46
6 phalp2_13871
7vJcf
 1 29,9% 254 3.655E-42
7 phalp2_28103
7vQh7
 9 34,5% 249 8.010E-41
8 phalp2_6225
6oM64
 106 34,9% 209 2.751E-40
9 phalp2_11417
eYHn
 21 26,3% 300 2.751E-40
10 phalp2_5937
6aZNZ
 614 25,7% 303 3.240E-39

Domains

Domains [InterPro]
Ami2
CW_7
CW_7
CW_7
Representative sequence (used for alignment): 3X7ua (326 AA)
Member sequence: A0A0U4K7B3 (369 AA)
1 326 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08230

Taxonomy

  Name Taxonomy ID Lineage
Phage Arthrobacter phage Gordon
[NCBI] 		1772298 Gordonvirus > Gordonvirus gordon
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KU160646 [NCBI]
CDS location
range 2678 -> 3787
strand +
CDS
ATGACCTTTTCATCGCTTATCTCGGGGACAATTCCTCACAACAACAAGTTCTCCTCCCGACAGGGTGTTCCTGTCAGCCGAATCATCCAGCACCACTTCGCTGGCTCTTCGGATGAACGACTTCGTAGTGCAACGGAACAGGCTTCCTCTCATTACGTCATCTACAATGACGGATCCATCTGGGGTCTCGTACCTGAAGAGTACCGGGCATGGACTTCGGGCTCGTTCGAAGCCGACGCTCCGTCGATTACCTACGAAGTTCAGAACAGCTCGTTCCAGATCAATGGTAACGACGATGATCCCAACTCGTGGGCTGTGTCTGACGCGGCTTACAACTCCATCGTTCTTCTGACTGCCGACATCGCTCGCCGTTATGGCTTCGGTGCGGTTACTCCGACGAATTACCAGGGTCACCGTCAGTGGTACTCGACGGCCTGCCCCGGTGGATTCCTTTGGGCTCGCATGGACAACACTCGTTCGTTGGCCAACAAGAATCTCAGTGGCGCAACTGTTCCGACACCTACTCCTTCAACCCCTCCGGTTGCTGGGAAGACTGTCTGGCAGCTTGCCGATGAAGTTCTTGCCGGACATCACGGTTCGGGGGATGATCGTAAGATCTCTCTCGGGATCCAATACGACGCCGTCCAGGCTGAGATCAATCGTCGCTATGGCGTTGTGGTCGTAGCACCTGCTGAGAAGACCGTTTCGCAGCTTGCTGATGAAGTTCTGGCTGGAGCTCATGGCAATGGAGAACAGCGTCGCGCTTCCCTCGGGAACCGTTTCGACGAAGTTCAAAATGAGATCAATCGTCGTCTCGGCGGCGGTGGGGTAGCACCTCAGGGTCTCAACATTGCACAGCTTGCTGACGCTGTTATGCGTGGCGAGTACGGTTCTGGTCAGGATCGCATCAACCGTCTCGGAGCAAACTACGATGCAGTCCAGCAGGAAGTCAACCGTCGTCTCGGCGGCAATGCTTCCCCGGTGGCAAACATCAATGCGCTCGCAGATGCAGTTCTCCGCGGTGAGTACGGCAACGGTGATGAGCGAGTTCGTCGCCTCGGAGCCAACTACGCAGCCGTTCAGGCTGAGATCAACCGCCGTTACGCATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000744c5a9_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (3X7ua) rather than this protein.
PDB ID
3X7ua
Method AlphaFoldv2
Resolution 81.18
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50