Protein

Protein accession
D3W0F3 [UniProt]
Representative
UUjd
Source
UniProt (cluster: phalp2_15234)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVNQAQVKQWIDTHVGKWVDFDGMYGAQCMDLAVQYAHDLWGFRLTGNAENLRNQALPAGWQRIKNSRGFVPQQGDIFVWYGTKHPYGHTGIVISATLNNYTALEQNMVTGKGQAADKAAINTRPYPSEFWGVVRPPITSGGNITPPDQNGTGGKLTAQRGTFKANSAVNIRRAPNTKTGTVAGVLKAGQTVNYDNIIDADGWRWVSWVGASGNRNYSAVRRLSDNFRTGLCY
Physico‐chemical
properties
protein length:233 AA
molecular weight:25623,4 Da
isoelectric point:9,69
hydropathy:-0,47
Representative Protein Details
Accession
UUjd
Protein name
UUjd
Sequence length
313 AA
Molecular weight
N/A Da
Isoelectric point
5,38273
Sequence
MKKQDAVNWAVKQIGKSIDADGAHGAQCMDEIVAFCKQHFDWHPTGDAIDLSTQELPKGFQRIENTDEFVPQQGDIGILDSGEYGHTNIIVVANQQYYDSIDQNWYNASSNGSPAAFVQNHDYSEFWGVIRPPYEDAEKGITTESTKLQVINDSINYTMNKRVGSIDGVVIHNTAGSRTAVQDYNALSNTSVARYEAGVAHYYIDRFTIWRAIDTYRIAWHVADTYGNGHYLGYEVEESMSASNKDFMMNEQATFKQAAIDMLYYGIEPNTKTVKLHNQFVATACPHRXFRSHGCDYHYYGNNVYKGFIRDRV
Other Proteins in cluster: phalp2_15234
Total (incl. this protein): 13 Avg length: 296,9 Avg pI: 7,15
Protein ID Length (AA) pI
UUjd 313 5,38273
3e8Q7 463 8,84966
7lwUf 463 8,55142
7rdFp 463 8,74825
wv2E 358 8,31173
X2KRT0 238 5,79101
I4DSK2 238 6,29005
A0A1L7DQ41 236 5,61174
A0AAE8BI36 238 6,29602
A0AAX3YWN5 237 7,69784
A0AAX4JHM4 142 5,41360
A0AAX4PR86 238 6,29022
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_38184
7bpzP
 284 53,0% 294 1.470E-101
2 phalp2_25267
88emb
 10 28,1% 298 1.822E-26
3 phalp2_5002
1dLD0
 578 27,4% 237 1.935E-20
4 phalp2_2230
4k5IM
 1 25,3% 319 3.840E-19
5 phalp2_18952
3MmP0
 1 25,4% 306 3.732E-15
6 phalp2_29259
8IzI7
 88 21,1% 326 7.420E-12

Domains

Domains [InterPro]
Unannotated
Ami2
Representative sequence (used for alignment): UUjd (313 AA)
Member sequence: D3W0F3 (233 AA)
1 313 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Lactococcus phage 1358
[NCBI] 		741942 Whiteheadvirus > Whiteheadvirus wv1358
Host Lactococcus lactis
[NCBI] 		1358 Firmicutes > Bacilli > Lactobacillales > Streptococcaceae > Lactococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
GQ403788 [NCBI]
CDS location
range 20162 -> 20863
strand +
CDS
ATGGTAAATCAAGCACAGGTCAAGCAATGGATTGACACACACGTCGGCAAATGGGTCGACTTCGACGGCATGTACGGCGCGCAATGCATGGACTTAGCCGTCCAATATGCACACGACCTTTGGGGCTTTCGCCTTACAGGTAACGCCGAAAACTTGCGAAATCAAGCTTTGCCCGCAGGGTGGCAACGCATTAAGAATTCGCGCGGCTTCGTTCCACAGCAGGGCGACATCTTCGTGTGGTACGGCACTAAGCACCCGTACGGGCACACAGGTATCGTCATCAGCGCGACGTTGAACAACTACACCGCGCTCGAGCAAAACATGGTTACAGGTAAAGGACAAGCCGCTGACAAAGCGGCAATCAACACACGCCCTTATCCAAGCGAATTTTGGGGCGTTGTGCGCCCGCCGATTACTTCGGGGGGAAATATTACCCCACCCGACCAAAACGGCACAGGCGGCAAACTGACGGCACAGCGGGGTACATTCAAGGCAAACAGCGCCGTGAATATCCGCCGCGCACCGAATACAAAAACAGGAACAGTCGCAGGCGTCCTCAAAGCAGGTCAGACCGTGAACTATGATAATATCATTGACGCGGACGGCTGGCGTTGGGTTTCATGGGTCGGCGCTTCGGGCAACCGAAATTATAGCGCAGTCCGCCGCTTGTCTGACAATTTCCGAACAGGGTTGTGCTATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0003824 catalytic activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001cd91fa_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (UUjd) rather than this protein.
PDB ID
UUjd
Method AlphaFoldv2
Resolution 91.78
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50