Protein

Protein accession
S5M947 [UniProt]
Representative
8JpFB
Source
UniProt (cluster: phalp2_15149)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVAWRDDFIRINQYSRPGSSLTAVRKIILHYTANPGASAANHQRYFNNLTDRYASAHLFVDKIEAICIIPLNEVTYQANDGSYRGVEALKPNANFLSVGVEMCQEPDGSFHPDTVTRSVNVCADLCRKFGLSASDIVRHYDVTHKYCPGPYVDNAALFTAFKNRVSDVLGGGSGGNTGGGTTAPPTGGGSNGIGTAYITGTNVNLRSGPGTGYSILRQLNAGESYIVWAEVNGWLNLGGDQWVKNDSSFLRFERTSGGSTGGSTGGSGSTNAGKRVVSKVDGLNFYSRPTWDKTYVVGQCNAGEGFTIVTKVPVDDAYQYKVQNSKGATYYITASSTYVEVR
Physico‐chemical
properties
protein length:342 AA
molecular weight:36721,3 Da
isoelectric point:8,42
hydropathy:-0,33
Representative Protein Details
Accession
8JpFB
Protein name
8JpFB
Sequence length
347 AA
Molecular weight
37171,85760 Da
Isoelectric point
7,59746
Sequence
MVAWRDDFIRINQYSRPGSKLTAVRKLILHYTANPGASAANHQRYFNNLTDRYASAHLFVDKIDSICIIPLDEVAYQANDGSYRGVAALQPNANFLAIGVEMCQEPDGSFHPDTVTRTVNVMADLCRKFGLSASDIVRHYDVTHKYCPGPYVDNPALFTAFKNRVSDVLGGGSGGGTGGNTGGGTTTPPTGGGDGRIGVAYITGTNVNLRSGPGTGYGVLRQLNAGESYIVWAEQDGWLCLGGDQWVKNDSSFLRFERTSGGSTGGSTGGGVGDTNAGKRVVSKVDGLNFYSRPTWDKSYVVGQCNAGEGFTIVTKVPVDDAYQYKVQNSKGATYYITASSTYVEVR
Other Proteins in cluster: phalp2_15149
Total (incl. this protein): 23 Avg length: 349,9 Avg pI: 8,25
Protein ID Length (AA) pI
8JpFB 347 7,59746
2CkVT 363 8,94043
36F8z 345 8,41669
39zYB 346 8,11233
3zE5L 365 9,41685
4DXgL 345 8,12000
4E4Yb 345 7,62099
4E5TO 342 7,56927
4E69H 335 6,65189
4EqS1 342 6,98696
4Erh5 361 8,96892
746V0 361 9,19114
7BBsQ 359 9,30970
7c4Ib 357 8,61609
7qqwj 381 8,93301
7rnkm 361 9,36521
8JpG2 342 8,41669
cZZB 346 8,35970
A0A068EP83 347 7,59746
A0A7D3ULZ2 333 7,05096
S5M4J3 346 8,40985
A0A977XRB4 336 7,74121
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3935
78x1H
 58 42,7% 353 6.761E-63
2 phalp2_14020
81gAb
 48 38,4% 351 2.839E-61
3 phalp2_11676
1LRu7
 24 32,5% 261 5.791E-37
4 phalp2_19907
6axIZ
 11 33,9% 256 1.451E-36
5 phalp2_15067
7vQ2g
 6 29,3% 232 4.403E-30
6 phalp2_14662
5ObrM
 161 29,6% 307 5.961E-30
7 phalp2_11225
6wIAx
 104 31,0% 258 8.380E-27
8 phalp2_11198
68WMu
 78 24,4% 217 1.693E-25
9 phalp2_20199
83ZcC
 8 24,0% 274 7.583E-25
10 phalp2_22264
7pxLN
 64 29,0% 231 1.023E-24

Domains

Domains [InterPro]
Ami2
SH3_3
Unannotated
Representative sequence (used for alignment): 8JpFB (347 AA)
Member sequence: S5M947 (342 AA)
1 347 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage Shanette
[NCBI] 		1296656 Herelleviridae > Siminovitchvirus > Siminovitchvirus shanette
Host Bacillus cereus
[NCBI] 		1396 Firmicutes > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KC595513 [NCBI]
CDS location
range 79914 -> 80942
strand +
CDS
ATGGTAGCATGGAGAGACGACTTTATTCGTATCAATCAGTATTCTAGACCAGGTTCTAGTCTTACAGCTGTACGTAAGATTATCTTACATTACACTGCTAACCCAGGTGCTAGTGCTGCTAACCATCAACGTTATTTCAATAACCTAACTGACCGTTACGCATCTGCACATTTATTCGTGGATAAGATTGAGGCAATCTGTATTATCCCATTAAACGAGGTAACTTACCAAGCTAATGATGGATCATACAGAGGAGTAGAAGCATTAAAGCCTAATGCTAACTTCTTATCAGTTGGTGTGGAAATGTGCCAAGAGCCTGATGGTAGCTTCCACCCTGATACAGTTACACGTTCCGTAAATGTTTGTGCAGACTTATGCCGTAAGTTCGGTTTATCTGCTTCTGACATTGTGCGACACTACGATGTAACGCATAAGTACTGTCCTGGTCCATATGTAGATAATGCAGCATTATTTACTGCTTTCAAGAATCGTGTATCTGATGTATTAGGTGGCGGTTCTGGTGGAAACACTGGTGGGGGTACAACTGCACCTCCTACTGGCGGTGGAAGTAATGGTATCGGTACAGCTTACATTACAGGCACTAACGTAAACTTACGTAGTGGTCCAGGTACTGGCTATTCTATACTACGTCAGCTAAATGCTGGTGAAAGCTATATAGTTTGGGCAGAGGTTAATGGATGGCTAAACTTAGGTGGCGACCAATGGGTTAAGAACGATTCTTCATTCTTACGTTTCGAGCGTACTAGTGGAGGTTCTACAGGTGGTTCTACTGGGGGTTCTGGTTCTACTAACGCTGGTAAACGAGTAGTATCAAAAGTAGATGGCTTAAACTTCTACAGCCGACCTACATGGGATAAAACTTATGTTGTTGGTCAATGTAATGCAGGTGAGGGCTTCACTATTGTTACTAAGGTGCCAGTAGATGACGCTTACCAGTACAAGGTGCAGAACTCCAAAGGTGCGACTTACTATATCACTGCTAGCTCCACTTATGTGGAAGTTAGATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi00035ab471_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8JpFB) rather than this protein.
PDB ID
8JpFB
Method AlphaFoldv2
Resolution 82.62
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50