Protein

Protein accession
A0A220BZ23 [UniProt]
Representative
28Kwv
Source
UniProt (cluster: phalp2_796)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAKTQAEINKRLDAYAKGTVDSPYRVKKATSYDPSFGVMEAGAIDADKYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQNYGTGFKIHENKPSTVPKKGWIAVYTEGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSSGQQYENSLAKAGYARYANGIAHYYGSEGYVWEAIDAKNQIAWHTGDGTGANSGNFRFAGIEVCQSMSASDAQFLKNEQAVFQFTAEKFKEWGLTPNRKTVRLHMEFVPTACPHRSMVLHTGFNPVTQGRPSQAIMNKLKDYFIKQIKNYMNNGTSSSTVVKKGKTSSASTPATRPVTGSWKKNQYGTWYKPENATFVNGNQPIVTRIGSPFLNAPVGGNLPAGATIVYDEVAIQAGHIWIGYNAYNGNRVYCPVRTCQGVPPNQVPGVAWGTFK
Physico‐chemical
properties
protein length:495 AA
molecular weight:54864,3 Da
isoelectric point:9,59
hydropathy:-0,63
Representative Protein Details
Accession
28Kwv
Protein name
28Kwv
Sequence length
240 AA
Molecular weight
27300,81230 Da
Isoelectric point
9,72720
Sequence
MKTQSQINKRLRDYKNGVVDSPYRVKRWTSYDASFGAMEPGCIDKDRAYHAQCMDLAIDYVMWLTDNQTEMWGDAKSSIINKFPKGWKIVENKPSTIPKKGWIAVYTAGTYSRYGHIGIVYDGGNTSSFQILEQNWNGWANKKPSLRWDNYYGLTHFIVPPVAKEIEAPKKDAKSAPKQLVKKNTNIKVNANHIKGWTMTKRGGKPKGVVIHNDAGTMNSKQYYNNLVNADYNRLARGIA
Other Proteins in cluster: phalp2_796
Total (incl. this protein): 80 Avg length: 399,0 Avg pI: 9,49
Protein ID Length (AA) pI
28Kwv 240 9,72720
75Bnd 281 9,28843
7aTPJ 209 9,77774
8JNqH 284 9,32169
Db3g 173 7,75980
ym70 340 9,54043
H9A139 209 9,77774
A0A076YLJ5 117 8,92592
G0XM55 494 9,50304
A0A0D3MUT0 496 9,54043
A0A223G0Q5 267 9,46249
A1YTR6 284 9,32169
A0A220BYG2 495 9,53238
A0A2K9V4B1 496 9,61238
A0A0N9SKE2 487 9,70863
A0A075BF30 267 9,46249
A0A0E3T7A8 267 9,46249
A0A0E3X980 267 9,46249
A0A0H4TFI6 267 9,46249
A0A0H4TFT7 267 9,46249
A0A0U1WF03 495 9,50304
A0A1S6KVP4 267 9,46249
A0A1X9SIZ0 495 9,53289
A0A220BXX6 495 9,53289
A0A249Y173 495 9,53289
A0A2R4P8P5 495 9,50304
A0A2R4SAX3 267 9,46249
A0A2Z5YQJ1 495 9,56190
A0A3G1IX95 267 9,46249
A0A3G1LE45 495 9,53289
A0A3G1LVL2 267 9,46249
A0A3G1SV15 495 9,53289
A0A3G6V8P1 495 9,50304
A0A3T0IDH5 495 9,53289
A0A410T9F6 267 9,46249
A0A410T9X3 267 9,46249
A0A410TAR2 267 9,46249
A0A411BLH1 267 9,46249
A0A411BLN7 267 9,46249
A0A449C1G7 495 9,54043
A0A449C2H8 496 9,63133
A0A449C2I2 495 9,50304
A0A449C4M0 496 9,56732
A0A4D5ZFX8 267 9,46249
A0A4D6DVY4 267 9,46249
A0A4Y6E7D0 171 9,45830
A0A5B8R6G8 267 9,46249
A0A5C1K771 495 9,50304
A0A5J6D6I4 495 9,53289
A0A6B9J3F4 495 9,53289
A0A6N1W257 495 9,53289
A0A7D3V2D8 495 9,56190
A0A7G7WVA2 495 9,53289
A0A8E5NS87 267 9,46249
D3G7G7 495 9,50304
E5L6X9 267 9,42310
G2ZIU7 267 9,46249
I6W8P7 495 9,50304
I6WKT7 495 9,50304
I6X5A8 495 9,50304
I6XDJ0 495 9,50304
I6XKI4 495 9,55430
K7QML4 495 9,50304
K7R291 267 9,46249
D6QY02 495 9,50304
I6W949 495 9,50291
A0A2H4JC36 487 9,61522
A0A8E5KBK2 496 9,53289
A0A8S5VS84 487 9,57538
A0A977KL01 495 9,56190
A0AA47KXA5 496 9,53289
A0AA50ACP7 487 9,65454
A0AA94WUI0 495 9,64681
A0AAE9P3C8 495 9,53289
A0AAE9VR66 495 9,53289
A0AAF0FFI7 496 9,62869
A0AAX4FYY5 495 9,53289
A0AAX4FZP4 495 9,53289
A0AAX4J6B7 495 9,56893
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_27772
7kL3N
 52 46,9% 147 6.457E-42
2 phalp2_35297
6Ynr2
 1 29,4% 204 7.937E-18
3 phalp2_14804
73AW6
 35 26,9% 178 1.579E-11
4 phalp2_14461
4HE7L
 9 26,7% 161 6.350E-08
5 phalp2_10799
3WA57
 47 28,1% 153 1.532E-07
6 phalp2_26341
1gBcI
 110 27,4% 204 5.186E-05

Domains

Domains [InterPro]
CHAP
Disordered region
Representative sequence (used for alignment): 28Kwv (240 AA)
Member sequence: A0A220BZ23 (495 AA)
1 240 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage vB_Sau_S24
[NCBI] 		1981553 Herelleviridae >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KY794643 [NCBI]
CDS location
range 133431 -> 134918
strand -
CDS
ATGGCTAAGACTCAAGCAGAAATAAACAAACGTTTAGACGCTTATGCAAAAGGAACAGTGGATAGCCCTTATAGAGTTAAAAAAGCAACAAGTTATGACCCATCGTTTGGTGTAATGGAAGCAGGGGCAATTGATGCAGATAAATATTATCACGCACAATGTCAAGACTTAATTACAGATTATGTATTATGGCTAACAGATAATAAAGTTAGAACTTGGGGTAATGCTAAAGACCAAATTAAACAGAACTATGGTACAGGTTTTAAAATACACGAGAACAAACCTTCTACAGTACCTAAAAAAGGTTGGATTGCTGTTTATACAGAAGGTAGTTATGAGCAATGGGGTCATATAGGTATTGTATATGATGGAGGTAATACTTCAACATTTACTATCCTAGAGCAGAACTGGAATGGTTATGCTAATAAAAAACCTACAAAACGTGTAGATAATTATTATGGATTAACTCATTTCATTGAAATACCTGTAAAAGCAGGAACTACTGTTAAAAAAGAAACAGCTAAGAAGAGTGCAAGTAAAACTCCTGCACCTAAAAAGAAAGCAACATTAAAAGTTTCTAAGAATCATATTAACTATACAATGGATAAACGTGGTAAAAAACCTGAAGGCATGGTAATACACAACGATGCGGGTCGTTCTTCAGGACAACAATACGAGAATTCATTAGCTAAAGCAGGTTATGCTAGATATGCTAATGGTATTGCTCATTACTACGGTTCTGAAGGTTATGTATGGGAAGCAATAGATGCTAAGAATCAAATTGCTTGGCATACAGGAGACGGAACAGGAGCAAACTCAGGTAACTTTAGATTTGCAGGTATTGAAGTCTGTCAATCAATGAGTGCTAGTGATGCTCAATTCCTTAAAAATGAACAGGCAGTATTCCAATTTACAGCAGAGAAATTTAAAGAATGGGGTCTTACTCCTAACCGTAAAACTGTAAGATTGCATATGGAATTCGTTCCTACTGCGTGCCCTCACCGTTCAATGGTTCTTCATACAGGATTCAATCCAGTAACACAAGGAAGACCATCACAAGCAATAATGAATAAACTTAAAGATTATTTCATTAAACAAATTAAAAACTACATGAATAACGGAACTTCAAGTTCTACTGTAGTTAAAAAAGGTAAAACAAGTAGTGCAAGTACACCGGCAACTAGACCAGTTACAGGTTCTTGGAAAAAGAACCAATACGGAACTTGGTATAAACCGGAAAACGCAACATTCGTTAATGGTAACCAACCTATAGTAACTAGAATAGGTTCTCCATTCTTAAACGCTCCAGTAGGAGGTAACTTACCGGCAGGAGCTACAATTGTATATGACGAGGTAGCAATCCAAGCGGGTCATATTTGGATTGGTTATAACGCTTATAATGGTAACAGAGTATATTGCCCTGTTAGAACTTGTCAAGGTGTTCCACCTAATCAAGTACCTGGTGTTGCTTGGGGAACATTCAAGTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0046872 metal ion binding molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000b8d73d5_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (28Kwv) rather than this protein.
PDB ID
28Kwv
Method AlphaFoldv2
Resolution 87.38
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50