Protein

Protein accession
A0A2H4P9I7 [UniProt]
Representative
40uRC
Source
UniProt (cluster: phalp2_25234)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MTYQLFEGHTSKNYTPGHLANQVWGQGARRVESITIHWWGNYGQEFWSVENFLCVNTKPTSAHFVVQDGLVSCIVNPDDAAWHAGNPYGNTTSIGIECRPEATDGDYQTIAELVAYLRKIYGDVPLVHHYEWQSTACPGTYDLARIDRLSRGVNVTESIEGSMTVAEADRVIAYLKALASDGWTDAQGKKHPGFMFVIEANQQRIDNIPALVAKAVWDTPINRGTKENPVYVRALQDLANNGTDTARVMADTKALLERPAGGGGGTVDTEAVAQSVFEKVIAWLKR
Physico‐chemical
properties
protein length:286 AA
molecular weight:31513,9 Da
isoelectric point:5,48
hydropathy:-0,31
Representative Protein Details
Accession
40uRC
Protein name
40uRC
Sequence length
240 AA
Molecular weight
25969,40640 Da
Isoelectric point
9,46198
Sequence
MAVTYETRYSSPNYTPAASVPAVYGMARKVEYITIHHWGLTGQSHAGVVNYLCRANGNTSAHYVISGLLATCIVDPDNAAWHAGHATGNARSIGLELRPEATALDYQGAAEVIADLRAVYGNVPLVPHKKWHATACPGKWDLAKLDRMAKAITAARKAPAKPASKPAPKPAPIVKEPAVATDPKDNRKEYNVWAYRYGKVDPRQVFSILLDAARDAKEARETAQANAVKLDKILAHLGIK
Other Proteins in cluster: phalp2_25234
Total (incl. this protein): 5 Avg length: 258,6 Avg pI: 7,94
Protein ID Length (AA) pI
40uRC 240 9,46198
A0A514A4J4 286 5,47964
A0AA49IJY3 239 9,67021
A0AA49IM57 242 9,60168
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_7901
8LrgT
 13 50,2% 169 2.698E-60
2 phalp2_31556
4fe7g
 22 47,5% 185 2.997E-58
3 phalp2_15294
1dl82
 2 36,9% 211 1.436E-54
4 phalp2_16559
7s5Uq
 12 48,4% 161 2.150E-49
5 phalp2_13806
716PV
 1 37,7% 159 5.976E-36
6 phalp2_13827
7cMO8
 12 42,1% 159 3.433E-34
7 phalp2_28005
6RLJZ
 8 39,1% 148 1.054E-32
8 phalp2_38221
7wekT
 2 34,2% 184 1.717E-24
9 phalp2_33401
6T3Pt
 1 35,7% 151 4.312E-18
10 phalp2_32194
6VJSL
 13 28,1% 199 1.942E-12

Domains

Domains [InterPro]
Ami2
Disordered region
Representative sequence (used for alignment): 40uRC (240 AA)
Member sequence: A0A2H4P9I7 (286 AA)
1 240 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Arthrobacter phage Urla
[NCBI] 		2047867 Korravirus > Korravirus urla
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MG198779 [NCBI]
CDS location
range 18448 -> 19308
strand +
CDS
ATGACATATCAACTGTTCGAAGGTCATACTTCGAAGAACTACACCCCAGGCCATTTGGCCAATCAGGTCTGGGGTCAGGGTGCGCGCCGGGTCGAGTCGATCACGATCCACTGGTGGGGCAACTACGGGCAGGAGTTCTGGTCTGTTGAGAACTTCCTGTGCGTGAACACCAAGCCGACCTCGGCGCACTTCGTGGTGCAGGATGGTTTGGTGTCCTGCATCGTCAACCCGGACGATGCCGCATGGCACGCGGGCAACCCGTACGGCAACACGACCTCCATTGGCATCGAGTGCCGACCGGAGGCCACGGACGGGGATTACCAGACCATCGCGGAGCTGGTGGCGTACCTGCGCAAAATCTACGGAGACGTACCGCTCGTGCACCACTACGAGTGGCAGTCCACGGCATGCCCGGGGACGTACGACCTCGCCCGTATTGACCGCCTCTCCCGAGGCGTCAACGTAACCGAATCAATTGAAGGGAGCATGACCGTGGCAGAAGCAGACCGCGTAATCGCATATTTGAAGGCTCTGGCCTCGGACGGCTGGACCGACGCCCAGGGTAAGAAGCACCCGGGCTTCATGTTCGTGATCGAGGCGAACCAGCAGCGGATCGACAACATCCCCGCGCTGGTGGCCAAGGCCGTTTGGGACACCCCGATCAACCGTGGCACCAAGGAGAACCCCGTCTACGTACGGGCACTCCAGGACCTCGCGAACAACGGCACGGACACTGCCCGTGTCATGGCCGACACCAAGGCCCTCCTTGAGCGGCCTGCAGGTGGCGGGGGCGGCACCGTGGACACGGAGGCAGTCGCCCAGTCCGTGTTCGAAAAAGTAATCGCTTGGCTCAAGCGCTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000ca3e18a_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (40uRC) rather than this protein.
PDB ID
40uRC
Method AlphaFoldv2
Resolution 79.25
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50