Protein

Protein accession
A0A223VZK2 [UniProt]
Representative
3LHkO
Source
UniProt (cluster: phalp2_31152)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
VAL
Probability: 72% (predicted by ML model)
Protein sequence
MTIKLKNGKLVSDVTNQVNQFKTTKKSSGVNQKRYVVVHYTAGENFDSDVRQLSSSPAKVSCHVVIGRNGQVAQIGSFDDIQWHAGVSQWDGINGLNSYSIGIELTNPGWLVCLDKHLPINERTKDGVRYQSASGRIYTQGLVFAKNPNVGSEIYGWVPYTTEQLTALREVIVALKQGFNSIREVVGHEQIAPKRKVDPGIGIIFPKKFLDQLNETTTDNTDDFYRGTDGKLYEYGFLDKTDRNLVAGKSGTVTTKGSALRIRDRPNAVAMVIGSYENGERVHIVDSEDGFYKTDKGYIASGYVKLDTPGFES
Physico‐chemical
properties
protein length:313 AA
molecular weight:34639,5 Da
isoelectric point:8,61
hydropathy:-0,45
Representative Protein Details
Accession
3LHkO
Protein name
3LHkO
Sequence length
219 AA
Molecular weight
24874,19370 Da
Isoelectric point
8,75856
Sequence
MQIINHKLVGEGIKHIPSPHHSSLVDVRYIIIHYTAGRGLDQTVKTFQSWHKTVSAHLVIGKDGRVVQMVPFDRAAWHAGRSRWGELTGMNKYSIGIELDNYGRLDQVEDGYQTWFGVRVDPGSVVQIVHQFGGPMCGWESYPDEQIDKLVHICAMLYYDYHVIEILGHDDIAPGRKLDPGPAFPMKRFKTSVWSAFKKRVLTQDVKSIRGASKITSEY
Other Proteins in cluster: phalp2_31152
Total (incl. this protein): 133 Avg length: 208,3 Avg pI: 8,30
Protein ID Length (AA) pI
3LHkO 219 8,75856
11maq 193 6,43209
13fR4 208 9,24117
13gpZ 205 9,84311
149fX 195 8,57708
152zt 201 8,37781
175wQ 196 7,77645
19xiW 176 7,07858
1Cid2 217 5,89190
1EY3e 225 9,99307
1FAuI 226 9,84601
1I2i7 162 6,09942
1QhC9 204 9,43367
1R0yb 200 5,78203
1YXgj 204 6,62142
1a3e7 162 9,26870
1bISK 162 9,38029
1bLus 230 9,28179
1bR6Y 162 9,23962
1bXnX 165 9,48841
1btDW 227 9,31783
1c0w1 232 9,01482
1c55J 165 9,23930
1cgDz 236 8,68178
1ci8U 234 9,47068
1cicG 227 9,28211
1dgYe 206 8,82290
1duXh 227 9,56100
1eVYC 155 9,67685
1eW4I 231 9,58343
1eW6W 157 9,22802
1eXC8 226 8,61667
1en3I 236 9,26464
1hdxG 187 9,25484
1lNuy 210 9,12461
1lQeZ 225 8,85539
1laVA 196 6,37986
1liu1 212 6,83110
1mPae 237 5,79231
1wucy 187 7,87425
1wxV3 187 7,87425
2ELJ3 204 6,82690
2Ggb6 187 9,25452
2I9kC 187 8,92612
2JUzM 162 8,99232
2QAhR 187 9,25452
2QtC1 170 9,75447
2QzdR 259 8,89872
2ZVwB 211 9,41898
2eQcL 164 9,18676
2ecJB 220 6,47228
2fYde 205 8,71247
2j2zU 165 7,77600
2lLHg 254 9,75157
2lfv9 272 8,22509
361wX 205 8,97569
37chq 205 9,66905
3MyVW 230 9,28159
3NvbA 214 9,73964
3NzZS 213 7,93549
3V58D 284 6,13181
3WWxp 206 9,60245
3ZBvV 216 9,09592
3hr8v 232 5,82556
3nq0x 200 6,21747
49ArQ 209 9,60303
49lz4 206 9,44373
4CSFT 206 7,22324
4Dwk9 225 6,70935
4GJsB 165 5,30498
4MoBN 265 5,18812
4SoYJ 189 9,94472
4Sp1J 187 7,87425
4UiRf 165 4,96713
4VpCF 285 8,97408
4VpCW 283 6,85890
4et9g 204 9,89501
4f1Io 225 9,55539
4f4Bl 164 9,07671
4gKI2 204 9,78761
4gaXi 190 6,42249
4idO6 227 9,54669
4jUST 164 6,17876
4kAdt 220 5,95999
4kVGm 226 8,45240
4kmOo 212 8,66747
4sT3y 162 8,74696
4tsBv 178 9,00187
4uFXw 283 6,85890
4vxZP 162 8,53724
5BjRc 206 6,65325
5Ew0Q 204 9,19385
5Hcqt 214 6,39560
5Ih5S 208 6,48234
5iqFh 234 9,22499
5jbwx 202 9,47493
6Qtpt 187 6,78324
6RU1p 206 7,27092
6RUQ8 206 6,74806
6RVRs 206 6,74795
6RgQF 270 5,32180
6VEWq 229 9,15298
7ImNT 256 9,66434
7IpE0 198 8,26622
7UpIf 187 8,92586
7VbYe 160 9,57563
7cOsy 259 9,54114
7dqua 227 9,13183
7lNNq 244 7,92479
7rZrn 201 5,57621
7wIHl 259 9,27096
7woyp 157 9,13422
7yiNC 227 8,95751
83Mc0 188 8,27789
8ake3 208 9,68091
8bZHt 187 8,95442
8eW5X 188 8,27789
8elAi 217 7,01646
8encY 216 6,44318
8kch4 267 9,57061
8qdJu 204 9,80185
AzR 162 8,76133
QPkf 161 9,13428
RrmL 193 10,00467
XMZY 215 6,37872
bjsw 207 5,96931
e5Mj 187 9,11784
k44A 215 7,02424
kZfl 257 9,05228
lVPm 204 6,89363
nVnH 187 9,49331
sRNL 206 9,35979
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_36096
6F3wF
 69 39,1% 199 3.693E-75
2 phalp2_22919
33qi6
 180 37,5% 197 2.214E-73
3 phalp2_4783
4YJpR
 19 36,3% 223 6.621E-65
4 phalp2_24979
ze0C
 8 36,4% 195 6.856E-56
5 phalp2_9775
84cP5
 1 35,1% 185 1.286E-55
6 phalp2_40199
2cSYZ
 15 32,8% 219 5.586E-54
7 phalp2_11760
45aqa
 242 37,1% 183 2.993E-51
8 phalp2_2346
4ToIc
 3 27,2% 198 6.919E-50
9 phalp2_31437
3j8r5
 5 36,8% 206 1.297E-49
10 phalp2_6728
24ioh
 33 32,5% 206 1.295E-46

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 3LHkO (219 AA)
Member sequence: A0A223VZK2 (313 AA)
1 219 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Agrobacterium phage Atu_ph04
[NCBI] 		2024263 Pootjesviridae > Rollinsvirus > Rollinsvirus ph04
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MF403007 [NCBI]
CDS location
range 4648 -> 5589
strand -
CDS
ATGACAATCAAGTTGAAGAATGGCAAGCTGGTAAGTGACGTCACTAACCAGGTCAATCAGTTCAAGACCACGAAGAAATCGTCCGGCGTCAATCAGAAACGATACGTCGTCGTACACTACACAGCCGGCGAGAATTTTGACAGTGACGTTCGTCAGCTCTCTTCGAGTCCGGCGAAGGTTTCGTGTCACGTCGTTATCGGTCGAAACGGACAAGTCGCTCAGATCGGCTCCTTCGACGACATCCAGTGGCATGCTGGCGTCTCACAGTGGGATGGCATCAACGGTCTGAACAGTTACTCGATCGGCATCGAACTCACGAATCCTGGCTGGCTGGTTTGCCTCGACAAGCATCTTCCTATCAACGAACGAACAAAGGACGGTGTCCGCTATCAGAGTGCAAGCGGCCGAATCTACACCCAGGGTCTCGTATTCGCGAAGAATCCGAACGTCGGCAGCGAAATCTACGGCTGGGTTCCGTATACCACGGAACAGCTCACAGCACTGAGAGAGGTCATCGTCGCTCTCAAGCAAGGTTTCAATTCGATTCGCGAAGTCGTCGGTCACGAACAGATCGCTCCCAAGCGCAAGGTCGACCCAGGCATCGGCATCATCTTCCCGAAGAAGTTCCTCGACCAGCTCAACGAAACGACGACAGACAACACTGATGATTTCTATCGAGGAACCGACGGCAAGCTCTACGAGTACGGTTTCCTCGACAAGACTGATCGTAACCTCGTTGCCGGAAAATCCGGAACGGTGACGACGAAAGGTTCTGCTCTTCGAATCCGAGATCGTCCGAACGCTGTGGCCATGGTCATCGGATCTTACGAGAACGGCGAACGAGTTCACATCGTCGACTCCGAAGACGGTTTCTACAAGACTGACAAGGGTTACATCGCTTCTGGATACGTGAAACTCGACACGCCTGGTTTCGAATCTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000bb9d0f2_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (3LHkO) rather than this protein.
PDB ID
3LHkO
Method AlphaFoldv2
Resolution 94.30
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50