Protein

Protein accession
A0A1W6DX33 [UniProt]
Representative
4YJpR
Source
UniProt (cluster: phalp2_4783)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 96% (predicted by ML model)
Protein sequence
MNISNHRLAGVDFVASPNISGMLNPQYIVMHYTAGWNDESAITTLKNPAAKVSAHVVIARDGKVTQMVPFNRVAWHAGPSKFDGKNGMNNYSIGIELVNIGFLREKPGAPGVYQLSTGSAWKDVPASNLAGYDLSLKAANKRIGGGTYVWPAYTKAQIDAAKEVVKALVGAYTIKDITTHEIIDTRGWKTDPGPAFPMGEFKPLIHIGNARNDNLEPNGTYVVTASALNVRKEANTGAGVLTVLRKGAEVAVVKDLGDWSYVEYAPGKFGYLTDKYLAAA
Physico‐chemical
properties
protein length:280 AA
molecular weight:30095,9 Da
isoelectric point:9,14
hydropathy:-0,16
Representative Protein Details
Accession
4YJpR
Protein name
4YJpR
Sequence length
262 AA
Molecular weight
29537,96660 Da
Isoelectric point
6,42112
Sequence
MNVTDHKLDGVRFVKTPNMGGTIEPKFIVLHFTSGWSFDGDVSTLSDPGSKVSAHIVVGREGELTQIVPFNRRAWHAGPSRSHGFDDINSHSIGIEVSNIGYLKIHGDGHYEDEYGNIITPEGEFTLDHHSPKRHTKTPPITWTEHYHPRLDKGRYVWEPFYPAQYAQLDVIIPALLKAYPSIKWIVTHEEIDTRGWKTDINGGGVFDLNRYLVLTGQAVKPSEHDEPVVVKATPVANPVPQSGPKPEKRYGMFGIPWLFWK
Other Proteins in cluster: phalp2_4783
Total (incl. this protein): 19 Avg length: 283,7 Avg pI: 7,73
Protein ID Length (AA) pI
4YJpR 262 6,42112
1XJeS 270 9,03874
3zzM2 282 6,76921
4ePbv 334 6,77335
4gTV2 279 6,17211
6AK54 294 7,84298
8OEl 279 9,10746
8iNvk 295 7,73513
A0A0F6R5Z1 317 9,25116
A0A1B0UZW5 263 6,13096
A0A2Z4QGE6 263 6,23901
A0A6H1Z6J9 263 7,99996
A0A7S5FXN6 263 8,02330
A0A976XMC3 263 7,10274
A0A9N6WU06 293 8,75244
A0AA96J3X1 262 6,75750
A0AAU8HYF9 314 8,81336
A0AAU8HYU9 314 8,81336
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_36096
6F3wF
 69 46,1% 206 4.675E-81
2 phalp2_31152
3LHkO
 133 40,9% 215 5.885E-65
3 phalp2_22919
33qi6
 180 35,3% 229 1.439E-52
4 phalp2_24979
ze0C
 8 35,0% 194 1.176E-37
5 phalp2_40199
2cSYZ
 15 28,0% 260 3.318E-33
6 phalp2_11760
45aqa
 242 33,4% 206 1.146E-32
7 phalp2_9775
84cP5
 1 32,8% 201 2.903E-32
8 phalp2_38722
YdMl
 4 27,4% 186 2.309E-26
9 phalp2_31437
3j8r5
 5 28,9% 190 3.141E-26
10 phalp2_10215
PQuN
 6 27,0% 211 1.987E-25

Domains

Domains [InterPro]
Ami2
Disordered region
Representative sequence (used for alignment): 4YJpR (262 AA)
Member sequence: A0A1W6DX33 (280 AA)
1 262 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Sphingobium phage Lacusarx
[NCBI] 		1980139 Lacusarxvirus > Lacusarxvirus lacusarx
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KY629563 [NCBI]
CDS location
range 29697 -> 30539
strand +
CDS
ATGAACATTTCAAACCACCGACTGGCGGGCGTCGATTTCGTCGCCTCGCCCAACATCTCGGGTATGCTCAACCCGCAATATATCGTCATGCACTACACCGCCGGTTGGAACGACGAGAGCGCCATCACGACGCTCAAGAACCCGGCCGCGAAAGTGTCGGCGCATGTCGTCATCGCACGCGACGGGAAGGTCACCCAGATGGTGCCGTTCAATCGCGTAGCGTGGCACGCCGGGCCGTCGAAGTTCGACGGCAAAAACGGCATGAACAACTATTCGATCGGCATCGAGCTGGTGAACATCGGCTTCCTGCGGGAGAAGCCGGGCGCGCCGGGCGTCTACCAGCTTTCGACCGGATCGGCGTGGAAGGATGTGCCCGCCAGCAATCTTGCGGGATATGATCTGTCCCTCAAGGCAGCGAACAAGCGGATCGGCGGCGGCACCTATGTCTGGCCTGCCTACACCAAGGCGCAGATCGACGCGGCGAAGGAAGTAGTCAAGGCGCTCGTCGGCGCCTACACGATCAAGGATATCACCACCCACGAGATCATCGACACTCGCGGCTGGAAGACCGACCCCGGCCCGGCGTTCCCAATGGGCGAGTTCAAGCCGCTGATCCATATCGGCAACGCCCGCAATGACAATCTGGAACCGAATGGCACCTATGTCGTCACCGCTTCGGCGCTGAACGTGCGGAAGGAAGCCAACACTGGCGCTGGCGTCCTTACCGTGCTCCGCAAGGGCGCTGAGGTCGCTGTCGTCAAGGACCTCGGTGACTGGTCCTATGTCGAATATGCTCCGGGCAAGTTCGGCTACCTCACCGACAAATATCTCGCAGCGGCCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000a1d3aab_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (4YJpR) rather than this protein.
PDB ID
4YJpR
Method AlphaFoldv2
Resolution 84.79
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50