Protein

Protein accession
A0A088F6M8 [UniProt]
Representative
3TI73
Source
UniProt (cluster: phalp2_25586)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MMKPRFITVHCSASKKGRYLTASEIRSMHVRPKSQGGNGWSDVGYHFIIRTDGATEEGRPLTRMGAGVAGHNEDNIHICLIGGLDSAGKPADTYEHIQKHALFGLIMDLCERFSIPFENVKGHRDWFGDTNGDGVIDHRDWLKECPCFTVATWLTGQLEELGY
Physico‐chemical
properties
protein length:163 AA
molecular weight:18205,4 Da
isoelectric point:6,29
hydropathy:-0,42
Representative Protein Details
Accession
3TI73
Protein name
3TI73
Sequence length
250 AA
Molecular weight
28120,65340 Da
Isoelectric point
8,75959
Sequence
MRPITKIILHCSANGPSCTFGAKEIRRFHTMPVSQGGRGWSDIGYHYVIKRDGTLEEGRPLEKAGAHTTGYNAESIGICLVGGVESDGRTPDDNFTTAQFETLARLIESLRSRWPKATIHGHSEFANKACPVFSVPKFLKSYGIEKDPSLVDWDSARWPHFKPGEFSALWGKGPMPLEWKKTLDALEDLRSMYGKPLYLERTSWQDNRLTCDIRVPSDKHAIFIRWAMDAGFTSARTVTANAVRVYGVWE
Other Proteins in cluster: phalp2_25586
Total (incl. this protein): 8 Avg length: 191,5 Avg pI: 7,69
Protein ID Length (AA) pI
3TI73 250 8,75959
23Jfa 243 9,29971
40kpy 263 8,48741
A0A2H5BG42 146 9,17651
A0A2I7QYZ5 152 6,47904
A0A2I7RRS0 152 6,02433
A0A9E8G463 163 7,00213
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_7609
5IQgF
 2 28,1% 167 6.187E-17
2 phalp2_27876
8EUCV
 18 30,4% 161 1.535E-16
3 phalp2_7337
4jq41
 32 28,9% 183 2.813E-16
4 phalp2_36432
11r0n
 207 27,3% 179 6.550E-10

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 3TI73 (250 AA)
Member sequence: A0A088F6M8 (163 AA)
1 250 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Vibrio phage VpKK5
[NCBI] 		1538804 No lineage information
Host Vibrio parahaemolyticus
[NCBI] 		670 Proteobacteria > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio > Vibrio harveyi group

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KM378617 [NCBI]
CDS location
range 48869 -> 49360
strand -
CDS
ATGATGAAGCCTCGATTCATTACAGTTCACTGCAGCGCATCTAAGAAAGGGCGTTATCTGACGGCTTCAGAAATCCGATCCATGCATGTTAGGCCTAAGTCACAAGGCGGTAACGGTTGGTCTGATGTGGGCTACCACTTCATTATCCGTACCGACGGTGCCACCGAAGAAGGGCGACCACTAACCCGTATGGGGGCTGGCGTTGCAGGCCATAACGAAGATAATATTCATATCTGCCTAATCGGTGGATTGGATAGTGCAGGTAAACCAGCAGATACCTACGAGCATATCCAGAAGCATGCCTTGTTCGGGCTGATCATGGACTTATGTGAACGCTTCTCGATCCCATTCGAAAACGTCAAGGGGCACAGAGACTGGTTTGGTGATACAAACGGTGATGGCGTTATCGACCATCGCGACTGGTTGAAAGAGTGCCCTTGCTTCACTGTGGCCACTTGGTTAACTGGTCAGCTGGAAGAGCTAGGCTACTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
A0A088F6M8
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (3TI73) rather than this protein.
PDB ID
3TI73
Method AlphaFoldv2
Resolution 78.61
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50