Protein

Protein accession
A5X9I5 [UniProt]
Representative
7tL4I
Source
UniProt (cluster: phalp2_22274)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MPRSDCHPQMAAFLDLLAYAEGTKGLGDDGYNKLVNPAGFFQDYREHPDVLVRVNPTLHSTAAGRYQFLSRHWRHYQAALGLPDFGPVSQDTWAIQLIRERKALDDVIKGRIPQAISKCANMWASLPGAGYGQREHKLADLLAKFTEFGGVLA
Physico‐chemical
properties
protein length:153 AA
molecular weight:17024,2 Da
isoelectric point:7,83
hydropathy:-0,32
Representative Protein Details
Accession
7tL4I
Protein name
7tL4I
Sequence length
234 AA
Molecular weight
25710,56850 Da
Isoelectric point
8,53260
Sequence
MTALLRRGSRGEAVRALQQELNRQAGAGLVDDGVFGPVTERAVKAFQRSRGLTPDGLVGPATRGALGTAPKRPAPGQGEANLCAFLDMIAFAEGTDRYGEQDGYDVLVGGELFTDFSDHPNKRVWLPAYGIHSTAAGRYQILHRYWKHYQAQLGLPDFGPASQDLYAVQQIRERRAYDDVLAGRISDAIRKCANIWASFPGAGYGQREVAERELIAFYRRKGGRLEHETGEMTA
Other Proteins in cluster: phalp2_22274
Total (incl. this protein): 47 Avg length: 164,9 Avg pI: 8,96
Protein ID Length (AA) pI
7tL4I 234 8,53260
7lkU2 226 7,85388
8gJY6 226 9,37314
eYXz 217 6,22878
A0A3G3M753 154 9,29990
Q9MC90 160 9,01276
A0A6B9X2B7 159 9,24523
A0A7U3VCA8 159 9,39209
A0A411BAK3 154 9,29990
A0A411BF89 154 9,16555
A0A5J6TBM1 154 9,23730
A0A385EH56 154 9,16587
A0A7G5B9G5 163 9,16781
A0A4D6DYM8 154 9,29990
A0A2Z5HSB9 154 9,27985
A0A6B9LQW6 154 9,29990
B1GS82 154 9,29990
A0A3G8F5V3 159 9,13783
A0A1W6DY17 154 9,29990
A0A1W6JT17 162 9,78690
A0A173GBW4 154 9,16587
A0A0N9SIA1 168 8,90491
A0A1B4U3Y9 162 9,58408
A0A2P1MXF6 154 9,32105
H2BD99 160 9,01276
D5LH07 162 9,51626
A0A291AXJ7 154 9,41530
A0A4V1EYN7 154 9,29990
A0A411BGV2 154 9,29997
A0A4Y5TNB0 154 9,16587
A0A6B9WVP8 159 9,13783
A0A7D7JFY7 149 9,22634
A0A8E3UZB5 154 9,29990
A0A9E6MIT4 154 9,29990
A0A9E9L7J0 159 9,11668
A0AAE7X2R9 243 9,56519
A0AAE8BT32 154 9,29971
A0AAE8XPV6 154 9,41504
A0AAF0D800 154 9,32079
A0AAU8GCH4 154 9,43761
A0AAU8GFI0 154 9,43761
A0AAU8GGH4 154 9,43761
A0AB39U1W8 154 9,27985
A0A6G5Y7G4 167 8,86913
A0AAE9HB56 176 4,94416
A0AAX4QJ35 176 4,94416
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_8549
1DMQs
 2433 55,2% 161 1.011E-62
2 phalp2_15310
1iZB6
 260 56,0% 148 1.256E-58
3 phalp2_14090
8oyxl
 17 55,9% 152 3.223E-58
4 phalp2_4748
7I1R1
 83 55,6% 158 1.722E-55
5 phalp2_14292
3zJio
 170 52,2% 157 1.005E-47
6 phalp2_10185
wALc
 11 44,7% 143 2.601E-36
7 phalp2_29942
8nkTU
 3 38,4% 182 4.312E-35
8 phalp2_24710
6nKSK
 5 37,6% 162 4.626E-33
9 phalp2_34051
89Glt
 18 35,4% 192 2.335E-30
10 phalp2_30604
6m7cL
 14 31,4% 156 1.070E-16

Domains

Domains [InterPro]
PG_1
Unannotated
Representative sequence (used for alignment): 7tL4I (234 AA)
Member sequence: A5X9I5 (153 AA)
1 234 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471

Taxonomy

  Name Taxonomy ID Lineage
Phage Aeromonas phage phiO18P
[NCBI] 		2913974 Peduoviridae > Bielevirus > Bielevirus phiO18P
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
DQ674738 [NCBI]
CDS location
range 22745 -> 23206
strand +
CDS
ATGCCGCGCAGTGACTGCCACCCCCAGATGGCCGCCTTTCTCGACCTGCTCGCCTATGCCGAGGGCACCAAGGGGCTGGGCGACGACGGTTACAACAAGCTGGTCAACCCGGCCGGGTTCTTCCAGGACTACCGCGAACACCCCGATGTGTTGGTGCGGGTCAACCCGACCCTGCACAGCACCGCTGCCGGACGCTATCAATTCCTGTCCCGTCATTGGCGCCACTACCAGGCGGCGCTCGGCCTGCCGGACTTTGGCCCCGTCTCACAGGACACCTGGGCTATCCAGCTTATCCGCGAGCGCAAGGCGCTGGACGACGTGATCAAGGGGCGCATCCCCCAGGCGATCAGCAAGTGCGCCAACATGTGGGCCAGCCTGCCCGGCGCCGGCTACGGCCAGCGCGAGCACAAGCTGGCGGATCTGCTGGCCAAGTTCACCGAGTTTGGCGGTGTGCTGGCATGA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016829 lyase activity molecular function None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
4.2.2.n2 None Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		HAMAP-Rule:MF_04109

Tertiary structure

PDB ID
A5X9I5
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi00015347c5_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7tL4I) rather than this protein.
PDB ID
7tL4I
Method AlphaFoldv2
Resolution 89.58
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50