Protein

Protein accession
S5M9Y4 [UniProt]
Representative
7bONi
Source
UniProt (cluster: phalp2_2700)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKPDDFFSKIAPIAIQEMKRTGIPASLTIAQGILESGWGGSELAVNANNWFGIKGVGPAGSYERDSPEEENGKKITRRSPFRKYHDWEDSVRDHSEFLLKPRYAKIINADWRTACHEVEKAGYATDSQYAEKLIKRIEEYQLYKYDQGVDRVVATPILIIDPGHGGTDSGAVGNGLKEKDLTLQISLYQFNRFKELGLPVAITRTTDITLKPSQRTGAVKQSRAMYCISNHINAGGGEGVEAIHSIHATDKLAKVLAHSVVNCGQKFRKAYSRQGNGGADYYFMHRETGAVDTTIMEYGFIDNTVDAERLKANWKIYAEAVIKAFCEYVGHTYKPKGGAAAMSDLDKSLEALVKAGVIQSPEYWKTNAVQGGKVKGENAATLIQNMAKKLGV
Physico‐chemical
properties
protein length:392 AA
molecular weight:43232,5 Da
isoelectric point:8,73
hydropathy:-0,45
Representative Protein Details
Accession
7bONi
Protein name
7bONi
Sequence length
442 AA
Molecular weight
48379,10390 Da
Isoelectric point
8,56722
Sequence
MIKQQAFIDTLAPAAVEDWKAYGVPASLTIAQAILESNWGTSELATRASNLFGIKGNGPAGTYTKVSDEYVNGKKIQKASGFRKYNSWIESIRDHTQFLLKPRYAKVIGSDWKTACREVYDSGYATDPAYPEKLKRIIEQYKLYEYDRKDGVQMRPILIIDAGHGGSDPGAVGNGLREKDLTLQISNYQYARFQALGVPVTLTRTSDVTLTPDQRTKLVRDSGVKYCLSNHINAGGGSGVEAIHSVFSSDKLAKALAQSVASCGMNFRRVFSKEGAGGKDYYFMHRETGSVETVILEYGFIDDAADALKLKDNWKTYAESVVKAFCEYIGVSYHLPTDLSPKGVDNVSIEVNGVQLPVKGYLQNGVSYLPVRAVSEAVGVSPEWDAATKQVKVNGRTLAATIVNGASYAPARELAAALGLQVEWEGSNRIVRINSSFYKTVE
Other Proteins in cluster: phalp2_2700
Total (incl. this protein): 21 Avg length: 402,8 Avg pI: 8,06
Protein ID Length (AA) pI
7bONi 442 8,56722
2VF4c 404 5,17521
2WCmA 434 8,62305
2dNH 432 9,30648
3eoKG 405 5,91367
76toR 340 9,68684
7cJiK 409 9,27624
7r7dc 391 6,61733
7srj9 394 7,11683
7teVY 399 8,71556
7tf1y 436 8,39657
7vXRg 392 7,16588
7w9Sd 474 9,12500
7wEEc 444 8,07004
7xH23 394 6,83235
7xZLZ 442 8,66154
7xZPj 410 7,61167
7xZWX 397 7,66976
8HzTA 392 8,72755
A0A9E7N069 235 9,17425
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_37594
45uQn
 106 34,4% 348 2.309E-60
2 phalp2_20406
39Yjh
 13 33,0% 345 1.465E-59
3 phalp2_37214
234pK
 1 31,8% 348 1.710E-52
4 phalp2_429
7BmmE
 20 29,4% 350 7.744E-50
5 phalp2_14098
7ooYL
 2 30,3% 372 3.563E-49
6 phalp2_24803
725t1
 19 28,3% 367 1.600E-41
7 phalp2_11791
82p55
 1 27,5% 352 2.459E-37
8 phalp2_40048
3v9yD
 1 20,8% 423 2.348E-24
9 phalp2_16521
71S8q
 9 24,5% 383 5.793E-22
10 phalp2_11993
37SWv
 2 22,5% 342 5.821E-21

Domains

Domains [InterPro]
GLUCO
Ami3
Cu_amine_oxidN1
Representative sequence (used for alignment): 7bONi (442 AA)
Member sequence: S5M9Y4 (392 AA)
1 442 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520, PF01832, PF07833

Taxonomy

  Name Taxonomy ID Lineage
Phage Brevibacillus phage Emery
[NCBI] 		1296660 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KC595516 [NCBI]
CDS location
range 24270 -> 25448
strand +
CDS
ATGAAACCGGATGATTTTTTCTCCAAAATAGCACCTATTGCTATCCAAGAAATGAAGAGAACCGGTATCCCTGCATCCTTAACCATTGCTCAAGGAATCCTGGAGTCAGGATGGGGAGGCTCCGAGCTGGCGGTTAATGCAAATAATTGGTTTGGGATTAAAGGAGTCGGACCTGCAGGTAGCTACGAGCGGGATTCCCCAGAAGAAGAAAACGGGAAGAAAATAACAAGGAGATCTCCTTTTCGCAAATATCATGACTGGGAAGATTCTGTGCGTGACCATTCAGAGTTTTTGTTGAAGCCACGTTACGCAAAGATTATTAACGCCGATTGGCGTACTGCTTGCCATGAAGTTGAGAAAGCGGGCTATGCAACTGATTCGCAGTATGCTGAAAAACTTATTAAACGAATCGAAGAATACCAACTTTACAAATATGACCAAGGAGTTGATAGAGTAGTGGCCACACCTATTTTGATTATTGATCCAGGACATGGCGGCACTGATTCAGGTGCAGTGGGCAACGGATTGAAAGAAAAGGATCTCACTCTCCAAATTAGTTTGTATCAATTTAATAGATTTAAAGAGCTAGGCTTGCCCGTAGCGATTACAAGGACAACAGATATCACTCTGAAACCATCACAACGTACAGGAGCGGTTAAACAAAGCAGGGCTATGTATTGCATTTCAAACCATATAAACGCTGGTGGAGGCGAAGGTGTGGAGGCGATCCACTCGATTCATGCCACTGATAAACTTGCGAAAGTGCTTGCCCATTCTGTTGTGAATTGTGGCCAAAAGTTCCGTAAGGCGTATAGCCGCCAAGGAAACGGAGGGGCTGACTACTATTTCATGCATCGAGAGACCGGCGCGGTAGACACCACAATTATGGAATATGGTTTTATCGACAATACGGTAGACGCGGAACGCTTAAAAGCTAACTGGAAAATTTACGCTGAGGCTGTTATCAAAGCATTTTGTGAATACGTTGGGCATACCTACAAACCAAAAGGAGGAGCTGCAGCTATGAGCGACTTAGATAAATCATTAGAGGCTCTTGTAAAAGCGGGTGTCATTCAATCACCTGAGTATTGGAAAACAAATGCTGTTCAAGGCGGGAAAGTAAAAGGTGAAAATGCCGCCACACTAATTCAGAACATGGCCAAAAAATTAGGGGTATAG

Gene Ontology

Description Category Evidence (source)
GO:0004040 amidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
upi00035aabb1_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7bONi) rather than this protein.
PDB ID
7bONi
Method AlphaFoldv2
Resolution 89.28
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50