Protein

Protein accession
B5AX34 [UniProt]
Representative
5EHW9
Source
UniProt (cluster: phalp2_9097)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAINKSGAAGGAAVVVAAIAALIMPSEGISLKAYKDPVGIPTICYGETQGVHYGDTKTKEECEAMLYKRIGDYLGPVDKMMPGLPDNRRIAYTDFAYNVGLGKLTERTKRNGKEIIGTSFVDLEKAGKWQESCERLNKYVYAAGKKLNGLVKRRAEEYQICMKSTEK
Physico‐chemical
properties
protein length:167 AA
molecular weight:18308,0 Da
isoelectric point:9,06
hydropathy:-0,37
Representative Protein Details
Accession
5EHW9
Protein name
5EHW9
Sequence length
197 AA
Molecular weight
21573,52070 Da
Isoelectric point
6,32069
Sequence
MNQNQRSASIKALIASVALATGISANTLTVTVNEVEELEGLSTTAYADMADPSLATACYGETEGIHFGDEFTPEECEVMLIKRLPDYIKPVKAAIPNAPDNRLIAYSLASWNLGRGLVMLRTTKCIEKKLISGECVKRVVIPGTSIADLEDAGNWQAACDRLHQFDRAGGKKIRGLAIRREKEYRICMGYLHGQQTQ
Other Proteins in cluster: phalp2_9097
Total (incl. this protein): 11 Avg length: 165,8 Avg pI: 8,89
Protein ID Length (AA) pI
5EHW9 197 6,32069
1KLKv 153 9,22925
1KUwh 153 8,61138
3GJzL 176 9,49498
3zGcT 149 8,88131
4xkLr 174 8,86887
6WQaU 152 9,69277
6dE1M 172 9,09567
7k5HK 164 9,50465
8Hj0X 167 9,05744
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_4305
8aoFg
 2477 32,0% 153 9.974E-32
2 phalp2_26525
83EoO
 66 30,9% 171 4.141E-27
3 phalp2_11002
4Mz75
 307 28,6% 157 3.684E-26
4 phalp2_9410
8Hj1q
 5109 28,9% 152 3.273E-25
5 phalp2_35864
4Lj4w
 95 28,2% 156 2.570E-23
6 phalp2_33109
4K52y
 18 30,0% 163 2.271E-22
7 phalp2_30973
1fTML
 10 27,1% 188 2.003E-21
8 phalp2_2632
6RhYr
 14867 27,7% 155 1.292E-20
9 phalp2_8851
2IyxA
 73 26,3% 171 1.134E-19
10 phalp2_126
5jCCA
 637 29,5% 149 9.932E-19

Domains

Domains [InterPro]
GH24
Representative sequence (used for alignment): 5EHW9 (197 AA)
Member sequence: B5AX34 (167 AA)
1 197 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF00959

Taxonomy

  Name Taxonomy ID Lineage
Phage Iodobacter phage PhiPLPE
[NCBI] 		551895 Iodovirus > Iodovirus PLPE
Host Iodobacter sp. CdM7
[NCBI] 		641420 Proteobacteria > Betaproteobacteria > Neisseriales > Chromobacteriaceae > Iodobacter >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
EU876853 [NCBI]
CDS location
range 5466 -> 5969
strand +
CDS
ATGGCAATAAATAAATCTGGCGCAGCAGGTGGGGCCGCCGTGGTAGTGGCGGCTATTGCGGCACTGATTATGCCTTCTGAGGGGATTAGCCTTAAGGCATACAAAGATCCAGTAGGCATTCCGACTATTTGCTACGGCGAGACGCAGGGCGTGCATTATGGCGACACTAAGACTAAGGAAGAGTGCGAGGCCATGCTGTATAAGCGCATCGGAGATTACCTTGGGCCAGTTGATAAGATGATGCCAGGTTTGCCGGATAATCGGCGGATTGCGTACACTGATTTCGCGTATAACGTGGGTTTGGGTAAGTTGACCGAGCGCACAAAACGAAACGGCAAAGAAATTATCGGCACGTCGTTTGTTGATCTCGAAAAAGCTGGAAAGTGGCAGGAGTCATGCGAAAGGCTGAATAAGTACGTCTACGCTGCCGGTAAAAAATTAAATGGTCTGGTTAAGCGCCGGGCGGAGGAATACCAGATATGTATGAAATCCACAGAGAAATGA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

PDB ID
upi00017bdc63_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (5EHW9) rather than this protein.
PDB ID
5EHW9
Method AlphaFoldv2
Resolution 93.35
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50