Protein

Protein accession
K4I410 [UniProt]
Representative
2UiYx
Source
UniProt (cluster: phalp2_31389)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 97% (predicted by ML model)
Protein sequence
MGRGAELLVPWAGIRTGVAVITVPQAAIDLAKRFEGFHRVAKNDPGRAHPYVCPAGYWTIGYGHLCDPKHPPITEAEAEAYLAQDLKAALAATLRYCPVLATEPEGRLAAIVDFTFNLGAGRLQTSTLRRRVNQRDWRGATQELRRWVYGGGRILPGLALRREAEVAHLDVDPLKSGAA
Physico‐chemical
properties
protein length:179 AA
molecular weight:19532,2 Da
isoelectric point:9,33
hydropathy:-0,17
Representative Protein Details
Accession
2UiYx
Protein name
2UiYx
Sequence length
255 AA
Molecular weight
27438,34710 Da
Isoelectric point
9,73307
Sequence
VVTGIGGGAIAVQYFWLQQWVDSVTGLVALGGLIFGCGLPGLAIVRWVFGGGSDHQLWRTVTRTLNNAGQLCGGPSGVVGRGVELLVPGGCVRPGAAVIAVPQAAIDLAKRFEGFHRVPRADPGRAHPYICPAGYWTIGYGHLCDPKHLPITEAEAEGHLAADLRTALNATLRYCPVLATEPEGRLAAIVDFTFNLGAGRLQTSTLRRRVNQREWVAAATELRRWVYGRGRVLAGLIGRRDAEAIWLLRNPPQRL
Other Proteins in cluster: phalp2_31389
Total (incl. this protein): 4 Avg length: 181,3 Avg pI: 9,57
Protein ID Length (AA) pI
2UiYx 255 9,73307
A0A6J5KUH0 149 9,33691
A0A6J5SGB2 142 9,90004
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6830
8n5Jv
 121 35,5% 180 2.611E-24
2 phalp2_1869
3V0OR
 7 36,1% 166 1.917E-22
3 phalp2_29484
1KOZ4
 4 32,0% 162 6.107E-18
4 phalp2_10624
2nrBp
 12 34,5% 165 1.120E-17
5 phalp2_34395
4g6xF
 2 30,8% 162 5.694E-13
6 phalp2_23797
1duFR
 19 30,3% 165 9.441E-10

Domains

Domains [InterPro]
Disordered region
GH24
Representative sequence (used for alignment): 2UiYx (255 AA)
Member sequence: K4I410 (179 AA)
1 255 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF00959

Taxonomy

  Name Taxonomy ID Lineage
Phage Acidithiobacillus phage AcaML1
[NCBI] 		1229761 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JX507079 [NCBI]
CDS location
range 48704 -> 49243
strand +
CDS
GTGGGCCGGGGTGCTGAACTTCTGGTTCCTTGGGCGGGTATTCGAACGGGTGTGGCCGTGATCACGGTTCCGCAGGCGGCCATCGACCTGGCCAAGCGCTTCGAGGGCTTCCACCGCGTGGCGAAGAACGATCCCGGCCGCGCCCATCCCTACGTCTGCCCGGCTGGGTACTGGACCATCGGCTATGGGCATCTCTGCGATCCCAAGCACCCGCCGATCACCGAAGCCGAGGCCGAAGCCTACCTCGCCCAGGACTTGAAGGCAGCGCTCGCTGCCACGCTGCGCTACTGCCCAGTGTTAGCCACCGAGCCGGAAGGGCGGCTTGCCGCCATCGTGGACTTCACGTTCAACCTCGGCGCGGGAAGGTTGCAGACGTCGACGCTGCGGCGGCGGGTCAATCAGCGGGACTGGCGCGGTGCAACGCAAGAGCTACGCCGCTGGGTATACGGCGGAGGCCGGATCTTGCCAGGTTTGGCTTTGAGGCGAGAGGCAGAGGTTGCGCACCTTGACGTCGATCCACTGAAAAGTGGAGCTGCGTGA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

PDB ID
upi000291ce0b_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (2UiYx) rather than this protein.
PDB ID
2UiYx
Method AlphaFoldv2
Resolution 78.60
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50