Protein

Protein accession
Q856D3 [UniProt]
Representative
8Lrse
Source
UniProt (cluster: phalp2_9416)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSRRGDVVWLPDILRSAGLPVQVADGANNRGHGDMADVWGIVDHHTGSDNASWQSIAFHPSLGLASQLHLSYGGLFTVCGVGIAWHAGNGSWPGLGTNNANPRTIGIEAANDGGGKPGRPHRADWPAVQYDHYVEGNAAILKFLNEPVTHSIGHKEWAGAAQGKWDPGGIDMNLFRKDIESAMRGAVPILNAIDEHAKVAKWLGKRITVPEKPTADGKGRWAEFENGTIYWSANTGAFAVPKHLLETYTALGWEQGELGYPIAPHAVIPDGGDVQAFQNGVLYRKYGQGGHVIKGAIGNRYRLNGWEAGFLGWPTSSEIKHGGMIYQTFEGGRIAFSPDGTIVLNKDNEFV
Physico‐chemical
properties
protein length:351 AA
molecular weight:37811,9 Da
isoelectric point:6,30
hydropathy:-0,34
Representative Protein Details
Accession
8Lrse
Protein name
8Lrse
Sequence length
351 AA
Molecular weight
37927,88880 Da
Isoelectric point
6,13198
Sequence
MSRRGDAVWLPDILRSAGLPVQVAEGANNRGHGDMADVWGVVDHHTGSDNASWQSIAYHPSLGLASQLHLSFSGLFTVCGVGIAWHAGNGSWPGLGTNNANPRTIGIEAANDGGGTPGRPHRADWPAVQYDHYVEGNAAILKFLNEPVTHSIGHKEWAGAAQGKWDPGGIDMNLFRKDIESAMRGAVPILNAIDEHAKVAAWLGKRLHPGEKPTADGKGRFAQFERGYIYWSAETGAFAIPSHLMETYAELGWEAGALGYPIAPHAVIPDAGDVQAFQRGVLYRKYGQPGHFITGAIGNRFRLNGWEAGWLGWPTTNETKQGGMLWQGFEHGRIAFSADGTVVLNERNEFV
Other Proteins in cluster: phalp2_9416
Total (incl. this protein): 65 Avg length: 368,2 Avg pI: 6,80
Protein ID Length (AA) pI
8Lrse 351 6,13198
1ca1Q 349 5,94282
1d7Jt 353 6,06520
1dMtV 507 6,19883
36G2C 355 6,05565
6Rpln 497 6,65212
6RtyI 359 5,88309
6Ru4Q 496 5,82488
753Vm 360 6,64524
7AGPk 344 7,81146
7AdvZ 354 8,76559
7PKzi 344 9,14911
7ehGk 370 6,41811
7erYE 355 5,81175
7hIsX 333 6,51963
7jGYX 369 8,31476
7jOj5 370 6,63813
7lDDX 345 6,89766
7loJt 351 6,31518
7p41B 347 5,72558
7r8X5 356 5,96221
7vnik 349 6,04451
7wDZG 342 7,88850
7xjrq 346 5,76327
7yyve 356 5,80522
7zSQt 384 5,91878
8HMRF 369 7,68875
8HMRN 375 6,56100
8HMRR 351 6,30148
8HMRU 346 8,95493
8Los9 381 6,14489
8Lpeq 344 6,75704
8LuFW 368 7,26928
8MAiB 370 7,22488
8MJmJ 355 5,78589
8MpIC 344 9,06163
8N07j 364 7,25222
8elp1 356 6,53258
8rQmq 356 5,68114
9wu 363 6,15961
A0A3S9UQK1 351 6,13198
A0A515MGQ6 369 8,31476
A0A4Y6EEE3 369 7,68875
A0A2H4PF03 375 6,56100
A0A2U8UKC2 370 7,22488
A0A2Z4Q3T0 375 6,60216
A0A345KRY4 375 7,17418
A0A3G8FFL9 369 7,20954
A0A3S9UR36 369 6,60216
A0A3S9URD9 375 6,60216
A0A4Y5NZK9 369 6,60216
A0A4Y6EPA5 375 7,17418
A0A514DFT8 369 7,20886
A0A649VXH4 375 6,60216
A0A6B9LJ18 375 7,17668
A0A7D5JJ38 369 7,72194
A0A7L7SRV7 369 6,60216
A0A7T0Q4U3 369 6,60216
A0A7T3KB06 375 7,72171
A0A4Y6EHX7 369 6,56100
A0A899INN0 368 7,20891
A0A8F2E770 375 6,83673
A0A8F3E9A2 369 6,56100
A0AAE7WKI3 375 6,60130
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_27885
8LlFs
 25 44,6% 352 6.147E-130
2 phalp2_7159
2QxCN
 1 25,6% 409 1.032E-39
3 phalp2_12497
QM59
 22 27,8% 248 5.764E-22

Domains

Domains [InterPro]
Ami2
LGFP
Representative sequence (used for alignment): 8Lrse (351 AA)
Member sequence: Q856D3 (351 AA)
1 351 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08310

Taxonomy

  Name Taxonomy ID Lineage
Phage Mycobacterium phage Barnyard
[NCBI] 		205880 Barnyardvirus > Barnyardvirus barnyard
Host Mycobacterium
[NCBI] 		1763 Actinobacteria > Actinobacteria > Corynebacteriales > Mycobacteriaceae >
Host Mycobacterium smegmatis
[NCBI] 		1772 Actinobacteria > Actinobacteria > Corynebacteriales > Mycobacteriaceae > Mycobacterium >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AY129339 [NCBI]
CDS location
range 31341 -> 32396
strand +
CDS
GTGTCTCGACGTGGTGATGTAGTTTGGCTCCCCGACATTCTCAGGAGTGCCGGGTTGCCCGTACAGGTGGCCGATGGTGCGAACAATCGCGGCCATGGCGACATGGCAGACGTGTGGGGGATCGTTGATCACCACACCGGAAGTGACAACGCTAGTTGGCAGTCCATCGCTTTCCATCCCTCGCTGGGTCTGGCCTCCCAGTTGCATCTGAGCTACGGCGGTCTGTTCACCGTCTGTGGTGTCGGCATTGCCTGGCACGCCGGAAACGGTTCGTGGCCGGGACTGGGAACGAACAACGCCAATCCCAGAACAATCGGTATCGAAGCTGCCAATGACGGTGGTGGAAAACCGGGCCGACCGCATCGAGCGGATTGGCCTGCGGTTCAATACGATCACTACGTTGAAGGTAACGCAGCGATCCTCAAGTTCCTGAATGAACCGGTCACACATTCCATCGGACACAAGGAATGGGCCGGAGCCGCACAGGGCAAGTGGGACCCCGGTGGCATCGACATGAACCTGTTCCGCAAGGACATCGAATCCGCAATGCGCGGTGCGGTTCCGATTCTCAACGCCATCGATGAGCATGCGAAGGTCGCGAAGTGGCTGGGCAAGCGAATCACGGTGCCCGAGAAGCCAACTGCGGACGGTAAGGGACGCTGGGCCGAATTCGAAAACGGTACGATCTACTGGTCGGCCAACACCGGAGCCTTCGCCGTCCCGAAGCATCTGCTCGAAACGTACACGGCTCTCGGTTGGGAACAGGGCGAGTTGGGTTATCCCATTGCGCCCCATGCGGTTATCCCAGACGGGGGTGACGTGCAGGCGTTCCAGAACGGCGTGCTCTACCGCAAGTACGGCCAAGGTGGACACGTCATCAAGGGAGCCATCGGCAATCGCTATCGGCTCAACGGTTGGGAAGCTGGATTCCTCGGCTGGCCGACCAGCAGTGAAATCAAGCACGGCGGCATGATCTATCAGACGTTCGAAGGTGGACGTATTGCGTTTTCACCTGACGGCACAATCGTTTTGAACAAGGACAACGAATTCGTATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000017d7da_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8Lrse) rather than this protein.
PDB ID
8Lrse
Method AlphaFoldv2
Resolution 92.07
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50