Protein

Protein accession
L0LC28 [UniProt]
Representative
3ATLj
Source
UniProt (cluster: phalp2_1848)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVSIKKDLVPAGLASQVTYKDGYNPCKYIVVHETANTKAGADAQAHANLQKNGNSREASWHYQVDDKGVIQSFDDRKQCWHAGSRVYNQNAIGIELCVNSGGDFKKTVDNAAELIKMLMNKYGIPVKNVLTHKETSGGKDCPHFLRSGSKGVTWAQLISKISSTSSSSGKTPSKPAKTPSKPAKSPSGSGSINFNTNSIVDFLSSAKLDSSFANRKKLAGKYGISNYSGTAAQNESLLAKLKSDFKSTSKPAPKPASKGDQKTNSIVDYLVSIKADSSFANRKKLADKYGISNYRGTAAQNSTLLKKIRGK
Physico‐chemical
properties
protein length:311 AA
molecular weight:33421,4 Da
isoelectric point:9,83
hydropathy:-0,62
Representative Protein Details
Accession
3ATLj
Protein name
3ATLj
Sequence length
366 AA
Molecular weight
41479,78920 Da
Isoelectric point
8,95513
Sequence
MVKMIDHLSKHDHYYSGTNQKKYIAIHETANRSVGAGAWSHARLQANGNSRQASWNWQVDDEIAIRSYPEDRRTWHAGDSEYGLNSISVEICVNRDGDYDKALQNAAELVAYLRKKFDIPWNRVISHKFITGKQCPAIMLADGRWDEFIKASDPKNAGKVTVASGGSEASAPQQYDKRKVITDDAEIKAGRSRGSETIGTPSKGYRLNIVEDQGSWTKVRWNYGTSDEPDYRNAYIATVDLEKKPAEYPHVALKRTDKHTRASHNAWVTLMAAVDYTDPDLGKNLQSWLNDLTDPRTGRGYYDLSKYRHDGIMGPIAVKGLQRKLYDTSAIGKKHLYYGVADGYRGPLTVRAEIDYLNWQRQFLTN
Other Proteins in cluster: phalp2_1848
Total (incl. this protein): 6 Avg length: 333,8 Avg pI: 9,57
Protein ID Length (AA) pI
3ATLj 366 8,95513
twj5 389 9,10534
A0A0A0PQA1 312 9,82680
A0A076G810 312 9,82016
A0AA96KRH0 313 9,86065
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_4901
5Zr0m
 117 27,7% 349 4.011E-26
2 phalp2_26995
4LbNv
 19 30,4% 256 5.427E-22
3 phalp2_3935
78x1H
 58 28,8% 246 1.085E-08

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 3ATLj (366 AA)
Member sequence: L0LC28 (311 AA)
1 366 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage phiAGATE
[NCBI] 		1204533 Herelleviridae > Agatevirus > Agatevirus agate
Host Bacillus pumilus
[NCBI] 		1408 Firmicutes > Bacilli > Bacillales > Bacillaceae > Bacillus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JX238501 [NCBI]
CDS location
range 131586 -> 132521
strand +
CDS
ATGGTAAGCATCAAAAAAGATCTCGTACCGGCAGGGCTTGCATCACAAGTTACGTATAAAGACGGTTACAACCCATGTAAGTACATTGTAGTTCACGAAACAGCTAATACAAAAGCAGGGGCAGATGCTCAAGCACACGCAAACTTACAAAAAAACGGCAACTCTCGAGAAGCTTCTTGGCATTATCAAGTAGATGATAAAGGTGTTATCCAGTCATTCGATGATCGAAAGCAATGTTGGCACGCAGGGTCTAGGGTATACAACCAAAACGCAATCGGTATAGAGCTTTGCGTAAACAGTGGAGGAGACTTCAAAAAGACTGTTGATAACGCCGCTGAGCTTATTAAGATGTTGATGAATAAATATGGTATTCCTGTTAAAAACGTTCTCACTCATAAGGAGACTAGTGGAGGGAAAGATTGCCCTCACTTCCTACGTAGCGGTAGTAAAGGCGTGACATGGGCACAACTTATCAGCAAAATCAGCTCAACTTCATCGTCAAGCGGAAAAACTCCATCTAAACCAGCTAAGACACCTAGTAAACCAGCTAAGTCTCCAAGTGGCTCCGGTTCAATTAACTTTAACACTAATAGCATTGTTGACTTCTTAAGCTCTGCAAAACTGGATTCTAGTTTTGCGAATCGTAAAAAGCTTGCAGGTAAGTATGGAATCTCTAATTATTCAGGCACAGCAGCTCAGAATGAAAGTTTACTGGCTAAACTTAAGAGTGATTTTAAATCTACTTCTAAGCCTGCACCTAAGCCTGCTTCAAAAGGAGATCAAAAAACGAACAGCATTGTTGACTATTTGGTTTCAATCAAGGCAGACTCCAGCTTTGCTAACCGTAAAAAGCTTGCGGACAAATACGGAATCTCTAACTACAGAGGTACCGCTGCCCAAAACAGTACGTTACTAAAGAAGATTAGAGGAAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0002a8890f_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (3ATLj) rather than this protein.
PDB ID
3ATLj
Method AlphaFoldv2
Resolution 89.21
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50