Protein

Protein accession
B1ABI9 [UniProt]
Representative
7vOVo
Source
UniProt (cluster: phalp2_23566)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTISSLTNIKKYEWQSSGHFGGITRKPIDMIVLHHNGGTGSNVVPNCWIEREASAHYQIENGQIINCLDEDITAWHCGAYGVDNNSHTIGIEHQNSTGAPDWLVSNENQEKSAQLVADIAKRYGIPLDRNHIVRHREMPNCNTDCSGGLDIDWVVTRAKQIAGQGGIPAPTPTPTPSNSNPVNPVVTVSYGLRQIGAGWLGTITDFNNSNGNGFAGNSNHEHDMLFASVSHGSLRYRVHTVKSGWLGWVNQGNKNDLVNGCAGNPNEAIDGVQFYYTTPNGEVYKQAYYRSQTSKRAGWLPSVEDDKDFAGILGEPLDRLQIAIRSSNPF
Physico‐chemical
properties
protein length:330 AA
molecular weight:36140,6 Da
isoelectric point:5,98
hydropathy:-0,53
Representative Protein Details
Accession
7vOVo
Protein name
7vOVo
Sequence length
241 AA
Molecular weight
26326,35330 Da
Isoelectric point
8,18479
Sequence
MTVSKLATVSKYSWQRGGNFGQIARKPINKLVLHHNAGTGSGVVPNCWVGRQASAHYQIENGTIISCLDEDITAWHCGASGVDNNSHTIGIEHQNSTGAPDWKVSAENQEKSAQLVAEICKRRGIPIDRNHIVRHREMPNCQTACSGGLDIDWIVNRAKEIAGEATASSSSKTKEINEEEIMKIIKLPNNATYYLVRSTGVTTLTAQEWIGVKRVLGFKDSDVDTVNQAEFDGIKSALGKK
Other Proteins in cluster: phalp2_23566
Total (incl. this protein): 2 Avg length: 285,5 Avg pI: 7,08
Protein ID Length (AA) pI
7vOVo 241 8,18479
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_11189
5Xg2q
 31 44,9% 167 1.777E-36
2 phalp2_24069
8amt4
 8 39,1% 166 1.124E-23
3 phalp2_7058
8iEHQ
 9 37,8% 156 7.131E-23
4 phalp2_6357
7sHfT
 17 33,5% 185 4.516E-22
5 phalp2_30952
173qx
 118 34,3% 198 1.135E-21
6 phalp2_17249
3TCUc
 1 35,6% 160 9.602E-19
7 phalp2_38224
7wFIi
 29 33,6% 187 1.102E-17
8 phalp2_8918
3eAb5
 8 29,1% 261 1.603E-14
9 phalp2_6225
6oM64
 106 37,0% 154 2.169E-14
10 phalp2_33971
40cAJ
 14 31,8% 179 5.361E-14

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 7vOVo (241 AA)
Member sequence: B1ABI9 (330 AA)
1 241 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Lactococcus phage asccphi28
[NCBI] 		503388 Salasmaviridae >
Host Lactococcus lactis
[NCBI] 		1358 Firmicutes > Bacilli > Lactobacillales > Streptococcaceae > Lactococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
EU438902 [NCBI]
CDS location
range 13093 -> 14085
strand -
CDS
ATGACAATTTCATCATTGACAAATATCAAAAAATATGAATGGCAAAGTTCAGGACATTTTGGAGGGATTACAAGAAAACCGATTGACATGATTGTTTTACACCATAATGGCGGAACAGGTTCAAACGTTGTTCCTAATTGTTGGATTGAACGGGAAGCGTCAGCGCATTATCAAATTGAAAACGGTCAAATTATAAACTGTTTAGATGAAGATATTACAGCTTGGCATTGTGGCGCTTATGGCGTTGATAATAATTCACATACCATTGGCATTGAACATCAAAACTCTACTGGTGCGCCTGATTGGTTAGTTTCTAATGAAAATCAAGAAAAATCAGCGCAATTAGTTGCGGATATTGCAAAACGCTACGGCATTCCATTAGATAGAAATCACATTGTCAGACACCGTGAAATGCCTAACTGCAATACAGATTGTTCAGGCGGTCTTGATATTGATTGGGTTGTTACCCGTGCAAAACAAATTGCGGGTCAAGGTGGAATACCTGCACCAACACCAACACCAACACCCTCAAACTCTAATCCAGTTAATCCAGTTGTTACTGTTTCCTACGGGTTGCGACAAATTGGTGCGGGTTGGCTTGGAACAATTACCGATTTTAACAACTCAAACGGAAATGGATTTGCTGGAAATTCTAATCATGAGCATGATATGTTATTTGCAAGCGTGTCACATGGTTCTTTAAGATACCGTGTTCATACTGTCAAATCGGGTTGGCTTGGTTGGGTTAATCAAGGAAATAAAAATGACCTTGTTAATGGTTGTGCGGGAAACCCTAATGAAGCAATTGACGGGGTTCAATTCTACTATACGACACCTAATGGAGAAGTTTACAAGCAAGCCTATTATCGCTCACAAACGTCTAAGCGTGCGGGGTGGTTACCTAGTGTAGAAGATGATAAAGACTTTGCGGGAATACTTGGCGAACCGCTTGACCGTTTACAAATTGCTATTCGTAGCTCAAACCCATTTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000165f86e_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7vOVo) rather than this protein.
PDB ID
7vOVo
Method AlphaFoldv2
Resolution 82.52
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50