Protein

Protein accession
A0A1B0T6L3 [UniProt]
Representative
4ToIc
Source
UniProt (cluster: phalp2_2346)
Protein name
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Lysin probability
100%
PhaLP type
endolysin
Probability: 97% (predicted by ML model)
Protein sequence
MRIINHRLKGVDFWPAHFTGGEIVPEIVVLHDTAGRLENGNSARYLASENTGKASVHFVVERDATVRQLVPTNCRANHAGRSNYHGREWCNGFSIGIEIVNPGRMTGDASEARAWWGETFSMRDEYDLAKVATPEHGDGVWMAYTEAQITAVISLLECLFRDVPTLTDITTHWYVSPGRKVDTNPLFPLEHIRARVLGRNDPADLEAEKGSDPSGDETVEIETHGDTLNMRRWPSFNPNVIAAIPDGTVVPVIRKGTFAGRKWLRVLYGGQEGWIVARYAAPITYHNT
Physico‐chemical
properties
protein length:288 AA
molecular weight:32116,7 Da
isoelectric point:6,00
hydropathy:-0,37
Representative Protein Details
Accession
4ToIc
Protein name
4ToIc
Sequence length
324 AA
Molecular weight
33678,75570 Da
Isoelectric point
7,75889
Sequence
MDASTLQRALAARGLYPHDEIDGDLGPKSEAAISALIAANLTRIPVFSATWSPERCRMAAEQIILKDDGFDPGTVDGLTGPRTEAAQKAWAARASKANGAQAKGASDAGAARPEPVSGGGLALAIKAGRLYRGGKPVQFIATPNMSGALKPEGIILHDTAGPSLSSATGWLSQSEAKASAHVVVDLDGTIVQMVPFDRVAWHAGPSSYKGRPNCNSFTVGIEIVNPGALSAAGKAWFHKTGQAGYSGIVHKATKAHGDGWWLPYTPEQIEAVTALCKALAAAYPSISFISTHWEISPGRKVDTNPLFPLGQVRGEVLGELVAGA
Other Proteins in cluster: phalp2_2346
Total (incl. this protein): 3 Avg length: 300,0 Avg pI: 6,51
Protein ID Length (AA) pI
4ToIc 324 7,75889
A0A1B0T6G1 288 5,76907
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_22919
33qi6
 180 51,9% 204 3.400E-78
2 phalp2_36096
6F3wF
 69 40,0% 212 2.901E-43
3 phalp2_9073
5weQ7
 5 28,7% 331 1.863E-14
4 phalp2_1053
74eYR
 26 28,1% 309 3.490E-12

Domains

Domains [InterPro]
Unannotated
Ami2
Representative sequence (used for alignment): 4ToIc (324 AA)
Member sequence: A0A1B0T6L3 (288 AA)
1 324 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Pelagibaca phage vB_PeaS-P1
[NCBI] 		1698522 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KT381865 [NCBI]
CDS location
range 25810 -> 26676
strand -
CDS
ATGCGGATCATCAATCACAGGCTCAAAGGCGTCGATTTCTGGCCGGCGCATTTCACCGGAGGCGAGATCGTTCCCGAGATCGTCGTCCTGCACGACACGGCTGGGCGCCTGGAGAACGGAAACTCCGCCCGTTACCTGGCATCGGAGAACACGGGAAAGGCGTCGGTTCACTTTGTGGTCGAGCGCGACGCAACCGTTCGGCAACTGGTGCCGACCAACTGCCGCGCGAACCATGCCGGGCGCTCGAATTATCATGGGCGCGAGTGGTGCAACGGTTTCTCCATCGGGATCGAGATCGTCAATCCGGGACGGATGACGGGCGACGCCAGCGAGGCCCGTGCCTGGTGGGGCGAGACCTTCAGCATGCGCGACGAGTACGATCTGGCGAAGGTCGCAACCCCGGAGCACGGTGATGGGGTCTGGATGGCCTATACCGAGGCCCAGATCACGGCAGTGATCTCGCTGCTCGAATGCCTGTTCCGCGACGTGCCGACGCTCACCGATATCACCACCCACTGGTACGTCAGCCCCGGCCGCAAGGTCGATACCAACCCGCTGTTCCCGCTGGAGCACATCCGCGCCCGTGTTCTCGGTCGCAACGATCCCGCCGACCTGGAGGCGGAGAAAGGATCGGACCCGAGCGGCGACGAAACCGTCGAGATCGAGACCCACGGTGACACGCTCAACATGCGGCGCTGGCCGTCGTTCAATCCCAACGTCATCGCGGCGATCCCTGACGGCACGGTTGTACCGGTCATCCGCAAGGGCACCTTCGCGGGGCGGAAATGGCTGCGGGTGCTCTATGGCGGTCAGGAAGGCTGGATCGTCGCCCGCTACGCCGCACCGATCACCTACCACAATACCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4ToIc) rather than this protein.
PDB ID
4ToIc
Method AlphaFoldv2
Resolution 87.62
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50