Protein

Protein accession
A0A0S2MY35 [UniProt]
Representative
86QiV
Source
UniProt (cluster: phalp2_10524)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
METYSKLTTSVNPNAMYCEPRQGRIEFIVIHHNATTNKDVAMSTWYTTSGNWTSAHYEITDNEIIGCVGENYTAYHAGGTGGSDVPTIPNVNHRSIGLEHVNSSGAPSWSVNDATLRNSAKLIADICQRYGLPINRNTIKAHNEVTATACPGGIDIDKLVRMAQESANGKQPEPSKPLPKPQKEDDKMFIYMKKQKNGNTEQWFVCGDKRMYLPTMTYVNEANALIKRYGGSTNQTVYNHDNFGLKMIEKAYTEVKV
Physico‐chemical
properties
protein length:257 AA
molecular weight:28630,9 Da
isoelectric point:7,66
hydropathy:-0,63
Representative Protein Details
Accession
86QiV
Protein name
86QiV
Sequence length
333 AA
Molecular weight
36097,68260 Da
Isoelectric point
9,22460
Sequence
MAGEVFSKLITSVNPKIMNASSRNGIKIDTIIIHHNATTNKDVAMDTWVKGGPAGTSAHYECTPDEIIGCVGEQYAAWHAGGTGGSDIPRISNPNQRSIGIENVNSSGAPSWSVDPRTIRNCAKLVADICKRYNIPLDRKHVLGHNEVTATACPGGINVDEVVRLAKEYAKGASDSNVAQEQKPSKPTTKTHDQIIAESKPKTVGNYVGKLEVFNELKWGIFRIAGWLVPINGAPYLNHGYVFWKEAGTGKEIGRCPSKGIMRNDVNKAYGLPNGLKFGLDGTLDIKKFAGKKVYPCLRRTNDKAGNSIAGKANGNGWIDIEFPEYVLTIPKR
Other Proteins in cluster: phalp2_10524
Total (incl. this protein): 42 Avg length: 298,1 Avg pI: 8,73
Protein ID Length (AA) pI
86QiV 333 9,22460
3MExO 297 8,64632
3MOZA 330 8,88395
3OGqU 297 8,86932
4Mh 330 9,24556
6Ii 330 9,26954
6azvk 333 9,39609
74AuX 335 9,33149
752dg 338 9,28894
752ek 338 9,35361
76j9Q 333 9,10843
7beKj 297 8,88286
7beLo 297 8,86919
7c2xd 328 9,24581
7oujW 330 9,09373
7oujf 297 8,33062
7oul1 308 9,35515
7r7Gs 330 9,26954
7r7t8 328 9,44270
7rBfT 330 7,72279
7scMo 335 9,36856
7u3kS 330 9,29481
7xnE7 330 9,32234
8nYgL 347 9,34722
8tQQ9 275 9,37527
CHiH 347 9,33742
A0A067XGV1 258 8,46355
A0A4D6DSZ3 258 8,17796
A0A7L7SHK2 258 7,64015
A0A1M4NDT8 258 7,62986
A0A4D6DRS8 257 7,63742
A0A4D6DTK3 257 8,16442
A0A4D6DT44 257 7,07670
A0A4D6DTU0 257 8,80143
A0A4D6DT39 258 8,47703
A0A4D6DSR9 257 7,65697
A0A4D6DT87 257 8,16442
A0A4D6DTJ0 258 8,46942
A0A9E7IFP8 257 8,47625
A0AAE7UV84 258 8,64935
A0AAU7L244 257 8,47625
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_1214
8MrbY
 36 56,3% 305 3.731E-93
2 phalp2_31265
8lSNT
 54 32,1% 323 5.369E-60
3 phalp2_10050
7qQCo
 2 30,1% 289 1.558E-55
4 phalp2_6798
8987b
 91 46,2% 216 2.500E-49
5 phalp2_26331
1cKvm
 65 47,2% 216 2.282E-46
6 phalp2_20487
3X7ua
 81 32,4% 219 7.795E-25
7 phalp2_8240
7x4iI
 9 27,6% 224 1.922E-24
8 phalp2_20231
8lqkG
 12 28,8% 239 7.605E-18
9 phalp2_15173
ovmV
 70 26,5% 222 6.789E-12

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 86QiV (333 AA)
Member sequence: A0A0S2MY35 (257 AA)
1 333 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Enterococcus phage vB_EfaP_IME195
[NCBI] 		1747288 Rountreeviridae > Copernicusvirus > Copernicusvirus IME195
Host Enterococcus faecalis
[NCBI] 		1351 Firmicutes > Bacilli > Lactobacillales > Enterococcaceae > Enterococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KT932700 [NCBI]
CDS location
range 9568 -> 10341
strand -
CDS
ATGGAAACTTATTCAAAATTAACAACTAGCGTGAACCCTAACGCTATGTATTGTGAACCTAGACAAGGTAGAATTGAGTTTATTGTTATACATCACAACGCGACAACTAATAAAGATGTTGCAATGTCAACGTGGTACACGACTTCAGGAAATTGGACTTCGGCTCACTATGAAATTACGGATAATGAGATTATTGGTTGTGTTGGTGAAAACTACACCGCTTATCACGCGGGAGGAACAGGCGGTAGTGACGTTCCAACTATTCCTAATGTAAATCATCGATCTATCGGACTAGAGCACGTAAACAGTTCAGGAGCCCCCTCATGGAGCGTTAACGATGCCACGCTTAGAAATAGTGCCAAATTAATTGCTGACATTTGCCAACGTTACGGTTTACCTATTAATAGAAACACTATTAAAGCTCACAATGAAGTCACTGCAACAGCATGCCCTGGAGGTATTGACATTGATAAGCTTGTGAGAATGGCTCAAGAATCAGCTAACGGAAAACAACCAGAACCATCAAAACCATTACCAAAACCACAGAAAGAAGATGATAAAATGTTTATTTATATGAAAAAACAAAAGAATGGAAATACTGAACAATGGTTTGTGTGCGGAGATAAACGCATGTACCTACCAACAATGACTTATGTAAACGAAGCAAATGCTTTAATTAAACGTTACGGAGGATCAACAAACCAAACGGTTTACAATCATGATAACTTTGGTTTAAAAATGATTGAGAAAGCTTATACGGAAGTTAAAGTATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (86QiV) rather than this protein.
PDB ID
86QiV
Method AlphaFoldv2
Resolution 90.23
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50