Protein

Protein accession
A0A068CC96 [UniProt]
Representative
8Fz4V
Source
UniProt (cluster: phalp2_9404)
Protein name
Putative N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTYRIPLDWLQSVKMSRVILHWSAGGWKASELDKEHYHFIVENDGNVVRGDHTVADNVNTADDDYAAHTRGCNTGAIGVSMACMAGAIESPFNAGKFPMTETQWNRAMEVIAHLASFYRIPVTPKTILSHAEVQANLGIQQAGKWDVARLPFDKATVGAKACGDKMRNQVQALL
Physico‐chemical
properties
protein length:174 AA
molecular weight:19155,6 Da
isoelectric point:6,64
hydropathy:-0,22
Representative Protein Details
Accession
8Fz4V
Protein name
8Fz4V
Sequence length
256 AA
Molecular weight
28260,67920 Da
Isoelectric point
7,05636
Sequence
mtipaswlpaatmkrvilhwsagthtasaldrahyhfilegdgnavrgdrsikdnegpirgnyaahtracntgsigvslacmagatespfrsgrypmmktqwdamidfvvelcrkyridptpetvlshaevennlgiwqrgkwdytrlsfdasikgaraigdrirtavrdrlaeglvereieeplggdraepvamalrgsttapwlnfrrapmgdiigglprgtsltiqdtddgwyqartpagylgwvsahyvcld
Other Proteins in cluster: phalp2_9404
Total (incl. this protein): 29 Avg length: 266,6 Avg pI: 7,38
Protein ID Length (AA) pI
8Fz4V 256 7,05636
1ozsV 271 6,52838
2B2Dj 256 6,24225
2B2zm 256 7,05636
2CiDn 357 7,11075
2G98 294 7,78535
3Yi0p 250 9,59543
3YikC 251 5,67631
3Zbkf 280 6,38924
4VXIr 308 7,84988
6RlF6 281 6,79888
6RyGY 248 6,59482
6VXe8 256 6,16961
726IM 258 8,58598
7Rdyc 257 9,19501
7lIZV 258 7,82010
7lIZu 258 7,70500
7lxqj 280 6,17234
7og3Z 278 6,96252
7pFgt 285 6,71788
7rNQe 279 6,59624
7w50p 319 6,63592
7wDNY 279 7,68437
7xExm 263 9,29571
8D0k3 232 9,51490
8jfg 248 9,64816
j9cS 306 6,86555
C4NTF2 193 7,06591
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_1021
6Rshc
 90 64,1% 170 7.214E-91
2 phalp2_19974
6PE0f
 7 55,2% 192 2.140E-82
3 phalp2_21458
8eHRi
 401 55,4% 175 4.014E-82
4 phalp2_4723
4RvuF
 8 54,3% 186 1.441E-75
5 phalp2_20043
1rrOC
 14 52,3% 172 2.735E-69
6 phalp2_22282
7wBZw
 14 41,9% 181 6.844E-54
7 phalp2_29754
1BARz
 1 31,2% 163 5.688E-21
8 phalp2_30152
3nf4u
 1 27,4% 164 2.630E-15
9 phalp2_24708
6km5N
 73 20,7% 241 1.284E-09

Domains

Domains [InterPro]
Ami2
SH3_3
Representative sequence (used for alignment): 8Fz4V (256 AA)
Member sequence: A0A068CC96 (174 AA)
1 256 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Rhizobium phage vB_RleM_PPF1
[NCBI] 		1498228 No lineage information
Host Rhizobium leguminosarum
[NCBI] 		384 Proteobacteria > Alphaproteobacteria > Rhizobiales > Rhizobiaceae > Rhizobium >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KJ746502 [NCBI]
CDS location
range 24720 -> 25244
strand +
CDS
ATGACCTACCGCATTCCGCTGGATTGGCTCCAGTCGGTGAAGATGAGCCGTGTCATCCTGCATTGGTCGGCCGGAGGCTGGAAAGCCTCGGAACTCGACAAGGAGCATTATCACTTCATCGTCGAAAACGACGGCAATGTCGTTCGCGGCGACCATACCGTGGCCGACAACGTCAACACTGCCGATGACGACTATGCTGCCCATACGCGCGGCTGCAACACCGGCGCGATCGGCGTGTCGATGGCCTGCATGGCGGGCGCAATCGAAAGCCCGTTCAACGCCGGCAAGTTTCCGATGACGGAAACTCAGTGGAACCGGGCGATGGAAGTGATCGCGCACTTGGCGAGCTTCTACAGGATCCCGGTGACCCCCAAGACCATCCTTAGCCACGCCGAGGTGCAGGCCAATCTCGGCATTCAGCAGGCCGGGAAATGGGATGTGGCGCGACTTCCCTTCGATAAGGCCACCGTCGGCGCCAAGGCCTGCGGCGACAAGATGCGCAACCAGGTGCAGGCGCTGCTCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8Fz4V) rather than this protein.
PDB ID
8Fz4V
Method AlphaFoldv2
Resolution 91.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50