Protein

Protein accession
R4JMV0 [UniProt]
Representative
7rrRH
Source
UniProt (cluster: phalp2_1093)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVKIIQALIPKQNRNRPGNRMKPLYITVHNTSNTGRGADAANHAAFVARASTGVSWHYTVDDQVIYQHLPLNENGWHAGDGRGTGNMKSIGIEICENAGANFEQAVKNAQWLIRKLMGDLGIPLSNVVPHKHWSGKECPRKLLNRWDGFKAGIASASTSQMTTAKPVKETSIKTISTRSTSKTNKVKKTYSLPAGILKVTKPLTKGSAVKAVQQALASIYFYPDKGAINNGIDGYYGPKTANAVSRFQMMHGLTPDGIYGPKTKETLSKVIES
Physico‐chemical
properties
protein length:273 AA
molecular weight:29716,7 Da
isoelectric point:9,98
hydropathy:-0,45
Representative Protein Details
Accession
7rrRH
Protein name
7rrRH
Sequence length
187 AA
Molecular weight
20271,34190 Da
Isoelectric point
10,00751
Sequence
MKSIGIEICENAGANFEQAVKNAQWLIRKLMGDLGIPLSNVVPHKHWSGKECPRKLLNRWDGFKAGIASASTSQMTTAKPVKETSIKTISTRSTSKTNKVKKTYSLPAGVLKVTKPLTKGSAVKAVQQALASIYFYPDKGAINNGIDGYYGPKTANAVSRFQMMHGLTPDGIYGPKTKKTLSKVIES
Other Proteins in cluster: phalp2_1093
Total (incl. this protein): 2 Avg length: 230,0 Avg pI: 9,99
Protein ID Length (AA) pI
7rrRH 187 10,00751
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_17898
EGF4
 130 30,4% 223 1.099E-12
2 phalp2_18579
7i83H
 2 31,4% 140 4.357E-11
3 phalp2_7264
3WBGP
 11 31,4% 181 1.090E-10
4 phalp2_36672
45lpM
 229 29,6% 192 1.925E-08
5 phalp2_38909
238Ru
 1 28,2% 191 7.208E-07
6 phalp2_12010
7G2oG
 1 27,1% 166 5.107E-04

Domains

Domains [InterPro]
Ami2
PG_1
Representative sequence (used for alignment): 7rrRH (187 AA)
Member sequence: R4JMV0 (273 AA)
1 187 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage PBP180
[NCBI] 		2558359 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KC847113 [NCBI]
CDS location
range 27384 -> 28205
strand +
CDS
ATGGTGAAGATCATTCAAGCACTCATCCCAAAACAAAATCGCAACAGACCAGGAAACAGGATGAAGCCGCTCTATATTACGGTGCATAATACATCGAACACTGGACGGGGGGCTGATGCGGCAAATCATGCAGCATTTGTTGCTCGGGCAAGTACCGGGGTTAGCTGGCACTACACGGTCGATGATCAGGTCATTTATCAGCATTTACCTTTAAACGAAAATGGCTGGCACGCAGGAGATGGCAGGGGAACCGGCAATATGAAATCAATTGGAATTGAAATTTGTGAAAATGCAGGCGCTAACTTTGAACAGGCTGTTAAAAACGCCCAATGGCTTATTCGGAAACTGATGGGGGATTTAGGAATTCCTTTATCTAACGTGGTGCCACATAAGCATTGGAGCGGGAAGGAGTGTCCGCGTAAGCTGTTAAATCGGTGGGATGGCTTCAAAGCTGGTATTGCATCAGCATCTACAAGTCAAATGACAACAGCTAAACCTGTGAAAGAAACATCTATCAAAACCATATCAACGAGATCAACATCTAAAACCAACAAGGTCAAAAAAACATACAGTTTGCCTGCAGGTATTTTAAAAGTGACGAAGCCTCTTACAAAAGGGTCAGCTGTAAAAGCTGTACAACAAGCCTTAGCATCGATTTATTTTTATCCTGATAAGGGAGCGATCAACAATGGAATCGATGGGTACTACGGACCCAAAACTGCGAATGCAGTGTCTCGATTTCAAATGATGCATGGGTTAACCCCGGATGGCATATATGGTCCAAAGACCAAAGAAACATTAAGTAAGGTCATAGAAAGTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi00032b64f4_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7rrRH) rather than this protein.
PDB ID
7rrRH
Method AlphaFoldv2
Resolution 82.24
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50