Protein

Protein accession
H9A141 [UniProt]
Representative
8JaN1
Source
UniProt (cluster: phalp2_8333)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNSKQYYNNLVNADYNRLARGIAHAYADRNGIWEAISEDRIAWHVSDGVQPGSGNFETYGIEVNQSMYVSDKDFLKNEQAALKFAAHKLKKWGLPANRNTVRLHNEFSYTACPHRSAKLHTGIDPTKQAWTKATQLKLKDYFIKQIRAYMKGDTPKVTTVKNKPGSASTPANRRDMNGWKINKYGTYYKSEVAHFTPNTPIKTHYVGPFRSCPVSGVLQPGQTVRYDTVCKQDDHVWISYTAYNGKDVWLAVRTWDKNTDSLGKLWGTIK
Physico‐chemical
properties
protein length:270 AA
molecular weight:30773,4 Da
isoelectric point:9,63
hydropathy:-0,74
Representative Protein Details
Accession
8JaN1
Protein name
8JaN1
Sequence length
121 AA
Molecular weight
13696,30800 Da
Isoelectric point
9,79882
Sequence
MTKRGRKPKGVVIHNDAGTMNSKQYYNNLVNADYNRLARGIAHAYADRNGIWEAISEDRIAWHVKDGVKPGSGNFEFYGIEVNQSMYAGDKDFLKNEQAALKFAAHKLKKWGLPANRNTVR
Other Proteins in cluster: phalp2_8333
Total (incl. this protein): 18 Avg length: 393,6 Avg pI: 9,44
Protein ID Length (AA) pI
8JaN1 121 9,79882
A0A0D3MVQ2 484 9,49840
A0A345AP02 484 9,47610
A0A345APK8 484 9,49840
W5RAT7 484 9,49840
A0AAE7FL77 484 9,51961
A0AAE7FLS9 484 9,51961
A0AAE7KD63 484 9,51961
A0AA49X3Q5 493 9,47165
A0AAE7KCG2 484 9,51961
A0AAF0D611 484 9,51961
A0AAX3Y1R2 484 9,52657
A0AAX3Y2R1 484 9,52657
A0AAX3Y380 484 9,49840
A0AAX4BH47 261 9,71991
A0AAE6LC10 67 8,02278
A0AAX4J7Z1 65 9,15653
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_15488
8diNj
 63 41,8% 122 2.870E-21
2 phalp2_36839
6Z1Os
 11 28,5% 119 5.895E-12
3 phalp2_33314
6pd1n
 5 35,7% 84 1.518E-08
4 phalp2_25038
13C0m
 57 28,0% 107 1.214E-06
5 phalp2_8874
2Yvfy
 25 30,9% 113 7.903E-06
6 phalp2_30401
4NIg7
 17 33,3% 81 1.474E-05
7 phalp2_17726
7f6JX
 68 29,7% 111 8.373E-04

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 8JaN1 (121 AA)
Member sequence: H9A141 (270 AA)
1 121 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage StB20
[NCBI] 		1147043 No lineage information
Host Staphylococcus capitis
[NCBI] 		29388 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JN700521 [NCBI]
CDS location
range 39802 -> 40614
strand +
CDS
ATGAATTCTAAACAATACTATAACAATTTGGTAAACGCTGATTACAATAGGTTAGCACGAGGTATAGCTCATGCATACGCTGATAGAAACGGTATTTGGGAAGCTATATCAGAAGATAGAATTGCTTGGCATGTTTCTGATGGCGTTCAACCAGGTTCAGGTAATTTTGAAACTTATGGAATTGAAGTTAATCAATCAATGTATGTAAGTGATAAAGATTTCCTTAAAAATGAACAAGCAGCTCTTAAATTCGCAGCGCATAAACTTAAAAAGTGGGGGTTACCAGCTAACAGAAATACTGTTCGTTTACACAACGAATTTAGTTATACAGCTTGTCCTCATCGTTCAGCTAAATTGCATACTGGTATTGATCCAACAAAACAAGCATGGACTAAAGCGACACAACTTAAGTTAAAAGATTACTTCATTAAACAAATTAGGGCGTATATGAAAGGTGATACACCTAAGGTTACTACGGTCAAAAATAAACCTGGTAGTGCTTCTACTCCTGCTAACAGACGAGATATGAACGGTTGGAAAATCAATAAGTATGGAACTTATTACAAATCAGAAGTAGCTCATTTTACGCCAAACACGCCTATTAAAACTCATTATGTTGGACCGTTTAGAAGTTGTCCTGTGAGCGGTGTATTACAACCCGGACAAACGGTAAGATACGATACCGTATGTAAACAAGATGATCACGTTTGGATTAGTTACACAGCCTACAATGGCAAAGATGTGTGGTTAGCAGTAAGAACATGGGACAAAAATACAGATAGTTTAGGTAAGTTGTGGGGGACAATTAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
H9A141
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi0002534cfe_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8JaN1) rather than this protein.
PDB ID
8JaN1
Method AlphaFoldv2
Resolution 97.74
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50