Protein

Protein accession
A0A4D6AAM3 [UniProt]
Representative
yopH
Source
UniProt (cluster: phalp2_2897)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVEKINVNLMNVGRLASIDFVVIHNDAGSMTPEQYINWLRNRNKELGIAHYYINRHTIARVIDTYNIGYHTGEWHSNTHSIGYEVCEGLKVGDKEFLENEDMTLMQATEDLLFYGLPINHQTVRLHHEFTPTTCPHRSLALHGGTTQSVKDYFVSRMKYFATLGKTVDEMLAKKGAPTSTPKPTIQPSSSKKSIDEVAREVLNGKWGNGAERKSALESAGYSYSQVQQRVNDLIYGTIPQTKSVDEVAKEVINGQWGNGQDRRNRLMGAGYNYDEIQRRVNELLG
Physico‐chemical
properties
protein length:285 AA
molecular weight:32236,9 Da
isoelectric point:6,84
hydropathy:-0,58
Representative Protein Details
Accession
yopH
Protein name
yopH
Sequence length
274 AA
Molecular weight
30703,39470 Da
Isoelectric point
4,72817
Sequence
MNAGQLDSIDFVVIHNDAGSMTPEQYVEWLRYRDKALGIAHYYCNKDTIARVIDTYNIGYHTGEWWSNTHSIGYEVCESMKVSDEDFLANEDMALMQATEDLIYYGLPINKQTVRLHHEFSPTSCPHRSLALHGGTTDSVKTYFVERMNYFATLGETVDEMLGNTSISEPSTSTNSVSTGDKSNEEIAREVISGAWGNGEDRVNRLTNAGYNASAVQEVVNRLLNGNYTSNNLDEIAQEVIQGKWGNGQDRVNRLTNAGYNYSEIQQKVNEILG
Other Proteins in cluster: phalp2_2897
Total (incl. this protein): 100 Avg length: 296,4 Avg pI: 5,08
Protein ID Length (AA) pI
yopH 274 4,72817
1HKi8 344 4,38225
1cwVK 344 4,59153
1cxtI 241 5,80942
1iXv2 307 6,71521
1j358 301 7,13599
1jLKl 283 4,79513
1kIN4 246 5,82227
1kJ8K 344 4,51383
3cdGM 236 6,89817
4SXSg 316 5,37978
5O87U 347 4,44745
5OVFV 283 4,70231
5X9SH 283 4,66969
5Y7QW 287 4,51111
65Bco 343 4,58443
65W4I 327 5,98761
69OY8 241 5,62538
69Y7w 197 7,09921
6Ybg3 283 4,85555
6ZHu9 348 4,51639
6ZWv6 245 4,67668
6Zald 344 4,51878
6Zdp5 283 4,75642
6ZqLE 241 6,04235
6ZydG 283 4,70231
6efO8 283 4,73778
6gXqR 246 5,81761
6hksD 337 6,70884
6hwDA 283 4,70231
6hzlV 347 4,33104
6iZmD 197 7,86245
6l6vm 287 4,80394
6lPaY 365 4,63445
6lbnI 246 5,82039
6mAzk 282 4,67429
6mZjl 346 4,43136
6t0Xs 283 4,70231
6th86 348 4,55300
703yD 246 5,93140
705xR 283 4,64081
7RfXK 295 6,84003
7UOib 241 5,97943
7XYtx 347 4,51383
7e2Tu 283 4,73778
7qHTq 283 4,79513
7yXDO 285 6,83679
84d1x 235 6,09276
86kmg 287 4,93672
88Vdq 346 4,46973
88W5n 241 5,59162
8bDXU 346 4,47774
8h9ow 336 4,53072
8qWQd 322 4,26562
8v6NI 241 5,87121
BVi7 239 4,91688
C4VU 283 4,84634
CIPB 283 4,80076
CLTR 345 4,53930
DBQQ 327 5,98960
Dw2F 347 4,56755
Exnb 241 5,80658
HJ1h 344 4,47547
HjMO 283 4,69293
HoAP 283 4,73778
Hrqn 283 4,74716
HznC 305 4,51293
JUBm 283 4,85555
JYio 283 4,74716
KMyZ 283 4,73778
Ka0b 347 4,56880
MXpC 283 4,70231
N9o9 346 4,39902
NWBE 264 4,57272
Naw0 347 4,44841
Nn5R 283 4,79513
NoJO 283 4,74716
NqIn 327 5,98960
NrAG 345 4,50838
NrfZ 283 4,79513
OA7R 346 4,50377
ODeT 334 5,36506
OKU9 362 4,66599
OU0T 283 4,79513
OmXq 283 4,78570
OqSW 239 4,80394
VNn9 197 7,06176
oAiu 283 4,70231
oNoB 293 4,43653
rotF 283 4,80940
wPaC 251 5,62470
wXwe 292 4,48905
wvWO 283 4,70231
wvXI 349 4,49889
xpgI 401 5,00146
yaSl 283 4,70231
ygi0 346 4,39089
yp1m 346 4,38663
yrig 346 4,57011
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_1607
7ZyR0
 345 34,9% 283 2.174E-49
2 phalp2_11417
eYHn
 21 33,0% 278 1.757E-42
3 phalp2_27991
13d0S
 3 35,6% 261 1.757E-42
4 phalp2_36896
7vCZc
 30 34,9% 203 3.236E-37
5 phalp2_14834
7s5S0
 47 31,3% 246 1.437E-31
6 phalp2_7784
7lEhL
 1 30,1% 196 2.011E-25
7 phalp2_20487
3X7ua
 81 28,0% 267 6.844E-25
8 phalp2_10280
1cMFf
 75 27,7% 285 3.070E-22
9 phalp2_6225
6oM64
 106 30,1% 202 6.424E-21
10 phalp2_14279
3nRBR
 57 33,4% 239 8.703E-21

Domains

Domains [InterPro]
Ami2
CW_7
CW_7
Representative sequence (used for alignment): yopH (274 AA)
Member sequence: A0A4D6AAM3 (285 AA)
1 274 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08230

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptococcus phage Javan261
[NCBI] 		2548078 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK448720 [NCBI]
CDS location
range 21079 -> 21936
strand +
CDS
ATGGTAGAAAAAATCAATGTAAACTTAATGAATGTTGGACGTTTAGCAAGTATCGACTTTGTGGTCATTCATAATGATGCAGGGAGTATGACACCTGAACAGTACATCAACTGGCTACGCAATCGAAATAAAGAACTAGGGATTGCCCATTATTACATTAATCGTCATACGATTGCGCGAGTAATTGATACCTATAATATTGGTTACCATACCGGTGAGTGGCATAGTAATACCCACTCTATTGGTTATGAAGTGTGCGAGGGTTTGAAAGTTGGAGATAAAGAATTTCTAGAAAATGAAGATATGACTTTGATGCAAGCAACAGAAGATTTACTGTTTTATGGCTTACCAATTAACCATCAAACCGTACGTTTGCATCATGAATTTACCCCAACGACCTGTCCACACCGTTCACTTGCACTTCACGGTGGCACGACACAGAGTGTAAAAGACTATTTTGTTTCTCGCATGAAGTACTTTGCGACATTAGGAAAAACAGTAGATGAGATGTTAGCGAAAAAAGGTGCTCCAACATCAACACCTAAACCGACAATTCAACCATCATCAAGCAAGAAGTCGATTGATGAAGTCGCACGAGAAGTGTTAAATGGTAAATGGGGAAATGGAGCCGAGCGCAAATCTGCGCTGGAGTCTGCAGGGTATTCTTACAGTCAAGTACAACAAAGAGTAAACGACCTTATTTATGGAACAATCCCGCAAACAAAATCGGTAGATGAAGTAGCAAAAGAGGTCATCAATGGTCAATGGGGGAATGGACAAGACCGTAGAAACAGACTCATGGGTGCAGGCTACAACTATGATGAAATTCAAAGACGAGTGAATGAGTTGTTGGGATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000225c971_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (yopH) rather than this protein.
PDB ID
yopH
Method AlphaFoldv2
Resolution 82.53
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50