Protein

Protein accession
Q4ZA79 [UniProt]
Representative
7BwRD
Source
UniProt (cluster: phalp2_30752)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MQAKLTKKEFIEWLKTSEGKQFNIDLWYGFQCFDYANAGWQVLFGYNLKGVGAKDIPSANNFNGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGVQQPGSGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGVNNSEFFINMI
Physico‐chemical
properties
protein length:213 AA
molecular weight:24046,0 Da
isoelectric point:8,79
hydropathy:-0,47
Representative Protein Details
Accession
7BwRD
Protein name
7BwRD
Sequence length
270 AA
Molecular weight
30986,59140 Da
Isoelectric point
9,42355
Sequence
MSFYFEEKEICFMRTKQEAIKWINNSIGKQYDFDGYFGFQCVDYSNAYWNYVTGGRLAGEGAKDLPFWNDFKGVATVYKNTPSFLAQGGDIVVWGSTFGNGFGHTGVVIDANSNYIVVVEQNWLLGGWTQGDARGGTGWEKATKRIHDYHYDMWFIRPKFKNPIKASVSNMVKKATSVTKKKAPAKTTKNKRIKVWSKTPHYKGVIKYNASLRQRSGSNFDNFNFNKEIGIVYQGETVYIFEEIQDEQGNIWCRTYSPSNNGWVHKHTIK
Other Proteins in cluster: phalp2_30752
Total (incl. this protein): 72 Avg length: 250,9 Avg pI: 8,56
Protein ID Length (AA) pI
7BwRD 270 9,42355
1nPRB 251 9,25200
5tFo 245 9,53283
72BTr 271 9,81546
764d0 225 9,47500
77Ydi 188 9,55230
77rUl 247 9,18483
78wub 251 9,26341
78xim 252 9,35960
7A39x 249 6,41220
7AML4 249 6,57692
7AMOQ 251 8,77281
7BWpg 251 8,92470
7QjSN 250 7,57177
7Xuoo 250 6,88305
7aoQR 217 9,57531
7c2j0 314 8,71137
7ddx3 251 9,31112
7gEbW 253 6,42846
7gaVr 321 6,19303
7jQuv 257 9,25200
7kG2I 251 9,02604
7mB6L 251 9,31099
7mszO 251 8,91445
7nZtU 254 9,14299
7qCTJ 249 8,52409
7qD7N 249 9,17090
7qDdx 249 8,85275
7r7rF 295 8,88544
7rBHW 296 7,14639
7rHH5 270 9,71224
7sMmc 249 8,22650
7sMos 249 8,88363
7saR1 265 9,57905
7sp1k 226 9,77768
7syMW 249 8,83444
7v3TH 251 9,31112
7wA4X 249 8,84340
7wA65 242 8,86229
7wlu6 254 9,44489
7wlw1 236 9,18412
7wlwP 254 9,64416
7wlzF 266 8,76462
7xPVh 334 7,61719
7yce1 192 8,96899
7yci8 251 8,76346
7zogF 296 7,10183
8JNqA 250 8,76965
8JNqC 249 6,19252
8JNqE 253 6,88050
8JNqr 249 5,57780
8JNqy 250 6,41322
8JNqz 250 7,58968
8JNtM 251 9,25200
8JjaW 252 5,66050
8JjaX 252 9,08380
8JjaZ 252 9,25742
8M3Cc 252 9,21455
8M5hl 249 6,41629
8M8Wy 250 6,41322
8MF7r 251 9,23131
8MKyB 253 5,56490
en5 283 8,77880
Q4ZDC4 213 8,46136
A0A7T8ETT3 265 9,92782
A0A7T8IXM9 265 9,90043
A0A8B6MGV6 265 9,96805
A0AAE9BMC0 265 9,92782
A0AAX4BBE7 175 9,56016
A0AAX4BIL6 124 9,69690
A0AAX4BJ03 242 9,63765
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_27772
7kL3N
 52 35,1% 168 1.776E-54
2 phalp2_36985
8MohX
 8 27,5% 272 5.328E-42
3 phalp2_796
28Kwv
 80 27,4% 171 4.392E-31
4 phalp2_25333
7ocj2
 56 30,3% 168 5.262E-24
5 phalp2_5002
1dLD0
 578 23,2% 224 9.807E-13
6 phalp2_3976
7uafd
 2 30,0% 170 1.101E-10
7 phalp2_34225
35z4C
 4 28,8% 177 6.391E-10
8 phalp2_30855
o6ig
 43 20,6% 252 2.755E-09

Domains

Domains [InterPro]
CHAP
Unannotated
Representative sequence (used for alignment): 7BwRD (270 AA)
Member sequence: Q4ZA79 (213 AA)
1 270 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Phietavirus X2
[NCBI] 		320850 Phietavirus >
Host Staphylococcus aureus
[NCBI] 		1280 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AY954968 [NCBI]
CDS location
range 23843 -> 24484
strand +
CDS
ATGCAAGCAAAATTAACTAAAAAAGAGTTTATAGAGTGGTTGAAAACATCTGAGGGAAAACAATTTAATATCGACCTTTGGTATGGATTTCAATGCTTTGACTATGCCAATGCAGGTTGGCAAGTCTTATTTGGCTACAACTTAAAAGGTGTAGGTGCCAAAGACATCCCAAGTGCTAATAATTTTAACGGACTAGCTACTGTATACCAAAATACACCAGACTTCTTAGCGCAACCTGGCGACATGGTTGTGTTCGGTAGCAACTACGGTGCTGGATATGGTCACGTAGCATGGGTAATTGAAGCAACTTTAGATTATATCATTGTATATGAGCAGAATTGGCTCGGCGGTGGCTGGACAGACGGTGTACAACAACCTGGCTCTGGTTGGGAAAAAGTTACAAGACGCCAACACGCTTACGACTTTCCTATGTGGTTTATCCGTCCGAACTTCAAAAGCGAAACAGCTCCACGATCAGTACAATCTCCTACACAAGCACCTAAAAAGGAAACAGCTAAGCCACAACCTAAAGCGGTAGAACTTAAAATCATCAAAGATGTAGTTAAAGGTTACGACCTACCTAAGCGTGGTAGTAACCCTAAAGGCGTAAATAATAGTGAATTTTTCATAAATATGATATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
Q4ZA79
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi0000505ba3_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7BwRD) rather than this protein.
PDB ID
7BwRD
Method AlphaFoldv2
Resolution 83.40
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50