Protein

Protein accession
H9A0Q2 [UniProt]
Representative
1lVmq
Source
UniProt (cluster: phalp2_10314)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MTEYWNGVPVRYDLLPIGTRRNGERLHTKDGKPKFAVIHDTGNLDSTAQQNVNWYKNTYNISWSQVASAHIFVDDKEAIICIPVTECAWHVMLNTTIDNAWYGADADYAAFGVEGCYFTDKKRSLKSLENTAKVMAYLTKFWKINYKNEMPGHQDIQFDKQDPGNLLAACGLGRDTHNFDLYVAKYMNETKVPVIKGKKAGNNAKKVTNTKSRPKTKSYQDAINYMYSLKGKYVDFDGMYGEQCMDLAVQYVYHITDGTIRMWGNAKDSILNVFPKGWQIVKNTPSYIPPVGAIGVCTTGIYQEYGHIYLVWDNSGGTNTQTVLEQNFDGNHNTPAKLRVDNFYGTTHYIVPSFINETYDVKKITKVNIQKPQAPVIKEKLPKNLTWSKEPYFKAKAGENGVTIREDVKNGYMKKTKLFYKANFSPFYVYEVREGWARVYSETANYWVRREDLIITEKLTPAGGKTKAVETVKAKGQTQQQQKIAKKSKPKVAVGQIPPEKLTWSRKRYFKAKSDALGVTICERHGGKGNYSWNKTNITYPQGQTFYVYEILDGWARVHGESDNYWVWHERLRITKVY
Physico‐chemical
properties
protein length:578 AA
molecular weight:66248,5 Da
isoelectric point:9,35
hydropathy:-0,63
Representative Protein Details
Accession
1lVmq
Protein name
1lVmq
Sequence length
362 AA
Molecular weight
N/A Da
Isoelectric point
8,98788
Sequence
MAEKWNGVPVRYDLLPIGTRRSGEALHTKNGKPSFAVIHDTGNPNTTAQDNVNYYKNTYNIAWSMVASAHIFVDDKEAIICIPVTEVAWHVMLNTTIDNQWYNADADYAAFGVEACYFTDKKRSLKSLDNAARIMAYLTKFWKINYKNEMPGHQDIQYDKQDPGNLLEACGLGRNTSIFDGYVAKYIDGVKVPKVSKKKAGNKGKNKEKPKSKPSTKDYQSAINYMYSLKGKFVDFDNRWAYQCMDLAVDYVYHITNGKVRMWGNAKDSILNIFPKGWKIVKNTPDYVPPVGAIGVCTYGIYQRYGHIYLVWDNSGGTNTQTVLEQNFDGNADTPAKLXXXXIIFMEQRITLCHHLLMKIMM
Other Proteins in cluster: phalp2_10314
Total (incl. this protein): 17 Avg length: 359,4 Avg pI: 8,81
Protein ID Length (AA) pI
1lVmq 362 8,98788
1ckxG 330 9,21912
1kFXq 315 9,40673
72BTs 296 9,66989
75Dj6 292 8,41224
75TCH 315 6,39947
7cYbp 292 8,40656
7rHH6 290 9,32672
7rxUD 475 9,30667
7rxW5 505 9,59517
7ryj6 475 9,27463
7sp1l 301 9,28346
8Ipq9 315 6,39793
8jCUe 320 9,25748
8jDMQ 357 8,79918
en6 292 8,60938
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_23551
7qsYO
 131 72,3% 343 3.720E-180
2 phalp2_8239
7wAa9
 4 34,7% 507 5.906E-104
3 phalp2_37223
23Tki
 1 23,2% 353 4.682E-10

Domains

Domains [InterPro]
Ami2
CHAP
Representative sequence (used for alignment): 1lVmq (362 AA)
Member sequence: H9A0Q2 (578 AA)
1 362 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage StB27
[NCBI] 		1147044 No lineage information
Host Staphylococcus hominis
[NCBI] 		1290 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JN700519 [NCBI]
CDS location
range 38194 -> 39930
strand +
CDS
ATGACAGAATATTGGAATGGCGTTCCTGTTAGATATGATTTATTACCGATAGGCACAAGACGAAACGGGGAACGGTTACACACTAAAGACGGTAAACCTAAATTTGCAGTAATACATGACACAGGGAATTTAGACTCAACTGCACAACAAAATGTAAACTGGTACAAAAATACTTATAATATTTCTTGGTCGCAAGTCGCATCGGCTCACATCTTTGTTGACGATAAAGAGGCAATCATTTGTATTCCAGTAACAGAATGTGCATGGCATGTTATGTTAAATACAACCATCGATAACGCATGGTATGGTGCTGATGCAGACTATGCAGCGTTTGGTGTAGAAGGTTGCTACTTTACAGATAAAAAGCGTTCTCTCAAGTCATTAGAGAATACAGCTAAGGTTATGGCGTATTTAACTAAGTTTTGGAAGATTAACTACAAGAATGAAATGCCAGGACATCAAGATATACAATTCGATAAACAAGATCCAGGTAACTTACTTGCAGCATGTGGTTTAGGTCGAGATACACACAATTTTGATTTATACGTGGCTAAATACATGAACGAAACTAAAGTACCAGTTATCAAAGGTAAGAAAGCTGGTAATAATGCTAAGAAAGTAACGAATACTAAATCAAGACCTAAAACTAAATCATATCAAGACGCTATCAACTATATGTATAGTTTGAAAGGTAAGTATGTAGACTTTGATGGTATGTATGGCGAGCAATGTATGGACTTAGCTGTTCAATACGTTTATCACATTACAGATGGCACAATTAGAATGTGGGGTAATGCGAAAGACAGCATCTTAAACGTATTCCCTAAAGGTTGGCAAATTGTTAAAAATACACCTAGCTATATCCCTCCAGTTGGTGCAATCGGTGTATGTACGACTGGTATTTATCAAGAATATGGCCACATCTACTTAGTGTGGGATAATAGTGGTGGTACAAATACACAAACTGTTTTAGAACAAAACTTTGATGGAAATCATAACACACCCGCTAAATTACGTGTAGATAACTTCTATGGCACAACTCACTACATCGTACCGTCATTTATCAATGAAACTTACGATGTTAAAAAGATTACAAAAGTGAACATTCAGAAACCACAAGCTCCAGTTATCAAAGAAAAGTTACCTAAAAACTTAACATGGTCTAAAGAGCCATACTTCAAAGCTAAAGCTGGAGAGAATGGTGTAACCATTCGTGAAGATGTTAAAAATGGTTACATGAAGAAAACTAAGCTATTCTATAAAGCGAATTTCAGTCCGTTCTATGTATATGAAGTTAGAGAAGGTTGGGCTAGAGTATATTCAGAAACTGCAAATTACTGGGTAAGACGTGAAGATCTAATCATCACAGAAAAACTTACGCCTGCTGGTGGTAAAACGAAAGCAGTTGAAACAGTTAAAGCTAAAGGACAAACGCAACAACAACAAAAGATTGCTAAAAAATCAAAACCTAAAGTAGCAGTAGGACAAATTCCACCAGAAAAATTAACATGGAGTAGAAAACGTTACTTCAAAGCAAAATCAGACGCATTAGGCGTAACGATATGTGAACGTCATGGTGGCAAAGGTAACTACTCATGGAATAAGACAAATATCACCTACCCACAAGGTCAAACTTTCTATGTATACGAAATCTTAGATGGTTGGGCTAGGGTACACGGAGAAAGTGATAATTACTGGGTATGGCATGAGCGTTTAAGAATAACAAAAGTGTATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0046872 metal ion binding molecular function None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
H9A0Q2
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi0002534c79_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (1lVmq) rather than this protein.
PDB ID
1lVmq
Method AlphaFoldv2
Resolution 80.45
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50