Protein

Protein accession
A0A0D4DD04 [UniProt]
Representative
73HNe
Source
UniProt (cluster: phalp2_18561)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MAEKWNGVPVKYDFLPIGTRRSGQPLTSKKPLFAVAHDTGNPETTAQTNVNYYKNTYMIDWSIVASAHIFVDDKECIVCIPVTEKAWHVLYNTPTDNQWYNADANDVAFGVEGSYFPSSQKRSRKSLDNMARVLAYLCNYWGIDYKTEVPGHQDIQDDKIDPGNLLEACGYSRNVKHLDKQIAKYINGVKPAPSKKLSTKTSKKPTPSPQSVVKYKQAIEYMHSLKGQYIDFDKEWAFQCADVVVDFIYHVTGGVRFYGNAKELHTKNAMPKGWKVVRNTRDYVPPICAIAIYTTGVYSRYGHTGLVWDNSGGTKTFTILEQNFDSKANSPAKLREDDYTGLTHFIVPDFADDSVDLTDIKEVQKTQRQSKSSIKLEQKPPLNLTWSNQPYFKAVADSEGVRICKPNHNNVMVVTNEEYDPGDVFYVYEIRNGWARVYSPSNDGYVWYERLIVKDIYKTAGGKKFAKKDDKQAVAQRNILADTTGLKVNSIPPLNMKKSSKAKFRARVDYYGAALVKFRGKEWYTTNDIYKAGYNQFYVFEIKDGWCRVYSKNNNGWIWHDRLRITKVY
Physico‐chemical
properties
protein length:569 AA
molecular weight:65121,9 Da
isoelectric point:9,14
hydropathy:-0,61
Representative Protein Details
Accession
73HNe
Protein name
73HNe
Sequence length
308 AA
Molecular weight
35485,24880 Da
Isoelectric point
4,47791
Sequence
MSKTYLEEWNGVPVYTQFIPYGTRRTGQQLDTGKPIFAVYHDTGNPNSTAQQNADFYCNTYNEDWNSTSSAHFFVDEKECIITVPIDEKAWHVLYNTPTDNYYFGDDANDAAFGGELCYFPGDRERSLKALDNFARINAVLFDSWDIDHWHKAPGHQDIQDDKQDPGNALSACGYDRDAVNIIDELVQKYIDGTDESIETKEVVNQQSEDEEVVEKKPVGCKRVKIWSEEPYYRGTIKYDASLRERAGSSFDNYSFAREKDVLEAGTLVYIYEEIEDPQGNIWCRTYSPSNNGWLHKHTIEVEEEYID
Other Proteins in cluster: phalp2_18561
Total (incl. this protein): 82 Avg length: 312,4 Avg pI: 7,29
Protein ID Length (AA) pI
73HNe 308 4,47791
1HYrz 305 4,41101
1aedm 302 9,38552
1jR5w 305 4,42062
1mGYG 299 9,12887
2dh1R 298 9,66441
3jQvT 306 4,34462
3kWg7 308 4,47791
3luOB 305 9,30893
415Tx 305 9,26354
5LiAH 305 9,30042
5QaEj 301 9,35058
5sUQ 299 9,33601
5tj4 299 9,23724
5tl7 305 4,23697
5tn5 305 4,42608
5tzv 304 9,16710
61bcS 305 4,42085
630lc 306 4,36878
65ifF 298 9,57209
6bzpn 299 9,18605
6o79x 299 9,28217
6ovjZ 299 9,29197
72PYI 305 4,46581
73Bcc 305 4,31365
73HHh 306 4,53430
73h2p 305 4,34707
7BWpq 315 5,24001
7BwRy 301 9,24743
7OR7t 298 9,48551
7Qk1v 305 8,96854
7QkBo 305 8,96854
7cFAz 306 4,40897
7ciXF 307 4,31888
7cnRy 306 4,50656
7cnhB 306 4,50656
7cnkk 306 4,54350
7e90X 305 4,56590
7eI2R 306 4,56351
7gpkj 305 4,52617
7kFZB 315 5,24001
7kG0M 315 5,55183
7kQIB 315 5,23251
7omIi 301 9,17928
7qCIe 301 9,18869
7qCJ8 301 9,20810
7qCOs 301 9,31028
7qCSf 301 9,11430
7qCUU 301 9,03262
7qDeN 301 9,17915
7qDmr 301 9,19824
7qDnq 301 9,36927
7rqnH 305 4,50429
7sMk9 301 9,24723
7sMsF 301 9,25716
7sZy4 301 9,24743
7sZy8 301 9,18857
7sfYH 301 9,30023
7u8pZ 301 4,56351
7wA75 313 9,11294
7yctH 317 8,89169
7zGaR 315 6,00586
7zGax 315 6,00586
81zDj 299 9,08922
8Ipp9 305 4,37111
8Ippa 305 4,43159
8Ipq2 305 8,96854
8IprQ 217 4,50798
8MrND 299 9,35199
8MrQL 295 9,23273
KFhd 298 9,54076
VUqy 305 4,42693
b1To 302 4,48485
ofZ8 298 9,51897
pM1V 298 9,47500
wuVz 298 9,60123
wucw 298 9,59033
z78j 298 9,52980
A0A1W6JQL2 569 9,16445
A0A1W6JNM6 569 9,07845
A0AAE7NZ80 305 4,35724
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_38757
1chSV
 7 33,3% 228 1.056E-37
2 phalp2_29706
1hMzx
 5 39,6% 194 9.150E-37

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 73HNe (308 AA)
Member sequence: A0A0D4DD04 (569 AA)
1 308 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage IME-SA4
[NCBI] 		1610872 No lineage information
Host Staphylococcus haemolyticus
[NCBI] 		1283 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KP735928 [NCBI]
CDS location
range 21893 -> 23602
strand +
CDS
ATGGCAGAAAAATGGAATGGCGTACCCGTTAAATATGATTTTTTACCGATTGGAACACGTAGAAGTGGGCAACCGTTAACAAGTAAAAAACCTTTATTTGCAGTAGCACATGATACAGGTAATCCTGAAACAACAGCACAAACAAACGTGAATTATTATAAAAATACGTATATGATTGATTGGTCAATCGTCGCAAGCGCTCATATTTTTGTTGATGATAAAGAATGTATTGTCTGTATTCCAGTTACCGAAAAAGCATGGCATGTTTTATACAATACACCAACTGACAACCAATGGTATAACGCTGATGCAAATGATGTAGCGTTTGGCGTGGAAGGTAGTTACTTCCCTAGTAGTCAAAAACGTTCTCGTAAGTCATTAGATAATATGGCACGTGTATTAGCTTATTTATGTAACTATTGGGGCATTGATTACAAAACTGAAGTACCAGGCCACCAAGACATTCAAGATGATAAAATTGATCCTGGAAACTTATTAGAGGCTTGCGGATATTCACGAAACGTTAAGCATCTAGATAAACAGATTGCTAAATACATTAATGGTGTTAAACCCGCACCGAGCAAAAAACTATCAACAAAAACAAGCAAAAAGCCGACACCTTCGCCGCAAAGTGTTGTTAAGTATAAACAAGCAATCGAATACATGCACAGCTTGAAAGGACAATATATCGATTTCGATAAAGAGTGGGCTTTTCAATGTGCTGATGTTGTTGTAGACTTCATCTACCATGTAACTGGTGGTGTACGTTTTTATGGTAACGCTAAAGAATTACACACTAAAAACGCCATGCCGAAAGGTTGGAAAGTGGTTAGAAACACACGTGATTACGTGCCACCTATTTGTGCAATCGCTATTTATACAACAGGCGTTTATAGTCGTTACGGCCATACTGGTTTAGTTTGGGACAATAGCGGTGGTACAAAAACATTTACAATCTTAGAGCAAAACTTTGATAGTAAAGCAAATTCGCCAGCTAAATTACGTGAAGATGATTACACAGGTTTAACACATTTTATTGTGCCAGACTTCGCCGACGATAGCGTTGATTTAACAGACATTAAAGAAGTACAAAAAACACAACGTCAATCTAAAAGTTCAATCAAGTTAGAACAAAAACCACCGTTAAATTTAACATGGAGTAATCAACCATACTTCAAAGCGGTTGCTGATAGTGAAGGCGTTAGAATTTGTAAACCTAACCATAATAACGTAATGGTGGTTACAAATGAAGAATACGATCCGGGCGACGTGTTCTACGTGTACGAAATTCGAAACGGTTGGGCTAGAGTTTACAGTCCAAGTAATGACGGTTATGTGTGGTATGAACGCTTAATTGTTAAAGATATTTATAAAACAGCAGGCGGTAAGAAGTTTGCGAAAAAAGATGATAAACAAGCAGTAGCACAACGTAATATTCTTGCAGATACAACAGGTTTAAAAGTGAATAGTATTCCACCATTAAACATGAAAAAGTCGTCTAAAGCTAAATTTAGAGCACGTGTCGATTATTACGGTGCAGCATTAGTTAAATTTAGAGGTAAGGAATGGTACACAACTAACGATATTTATAAAGCAGGATATAATCAATTTTATGTCTTTGAAATTAAGGACGGTTGGTGTCGTGTTTATTCTAAAAATAACAATGGTTGGATATGGCATGATCGTTTAAGAATTACAAAAGTGTATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0046872 metal ion binding molecular function None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0005d8abb7_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (73HNe) rather than this protein.
PDB ID
73HNe
Method AlphaFoldv2
Resolution 86.95
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50