Protein

Protein accession
U5P0A9 [UniProt]
Representative
8jDjT
Source
UniProt (cluster: phalp2_10564)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MQVLNSQRIAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTDYSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKEAGGVVNEVEL
Physico‐chemical
properties
protein length:158 AA
molecular weight:17793,0 Da
isoelectric point:8,69
hydropathy:-0,53
Representative Protein Details
Accession
8jDjT
Protein name
8jDjT
Sequence length
111 AA
Molecular weight
12444,13240 Da
Isoelectric point
9,55894
Sequence
MPQQLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV
Other Proteins in cluster: phalp2_10564
Total (incl. this protein): 69 Avg length: 155,6 Avg pI: 9,14
Protein ID Length (AA) pI
8jDjT 111 9,55894
73cwT 158 9,23692
7enHK 124 9,17219
7oOMZ 119 9,30255
A0A7G0Y2F4 158 9,20958
K7PKI0 158 9,20958
G8C7N4 158 9,20958
K7P7P0 158 9,37726
Q8SBE0 158 9,23679
A0A482IM96 159 9,61889
A0A653FRK9 158 9,23679
A0A291LBI6 158 8,90542
Q9MCT2 158 9,34929
A0A0M7Q8U4 156 9,45063
C6ZR65 158 9,23647
Q716B5 158 8,66470
K7P7A5 158 9,20958
K7P847 158 9,20958
S5FV07 158 9,03797
K7P7Y6 158 9,18444
A0A6B9X8F5 156 9,35348
K7PKX1 158 9,37726
A0A2P1CKX6 156 9,12990
A0A2S1GT50 158 9,41279
K7P890 158 9,34961
A0A3G8F2Y5 157 8,94449
A0A4D6DZ88 157 9,22544
A0A088CPU7 158 9,20958
A0A0C4Y1Z9 158 9,20958
A0A0F6R6B1 156 9,35348
A0A0F6TKN2 156 9,37262
A0A220NRL5 158 9,20958
A0A2D1GLJ2 158 9,20958
A0A3G3M745 158 9,20958
A0A4Y5TVV9 156 9,26071
A0A5Q2EZZ2 157 9,20958
A0A6B9X779 156 9,45063
A0A6C0R082 157 8,92470
A0A7D5JIZ6 156 9,45063
A0A7H0XDK9 156 9,43103
A0A7H0XDZ6 156 9,43103
A0A7H0XEA7 156 9,43103
A0A7L7ST14 158 9,20958
A0A7L7SWJ9 156 9,43103
A0A7U3VC53 158 7,85407
A5VW81 158 9,20958
B3RGM4 156 9,35348
G0ZT35 153 7,81655
G5DA94 158 9,20958
K7P6P0 158 9,20958
K7PGZ8 158 9,20958
K7PHB0 158 9,20958
K7PHF2 158 9,20958
K7PHP2 158 9,20958
K7PKV2 158 9,20958
Q77IT9 158 9,20958
A0A2Z2XQ31 156 9,45063
A0A2Z2XST2 156 9,45063
A0A6J5KH20 155 6,82599
A0A8S5VJY1 158 9,06608
A0AAD1Q9R7 156 9,35348
A0AAE7P5A5 164 9,62424
A0AAE7TQU0 157 8,98846
A0A6G5Y8R6 157 9,34219
A0A6J5KJN5 155 6,39833
A0A9E8Z050 157 9,57750
A0AAE8BGF3 158 9,43148
A0AAU8BSU7 156 9,26096
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_15310
1iZB6
 260 53,2% 109 9.678E-43
2 phalp2_15532
8vLOB
 4 53,7% 93 1.167E-37
3 phalp2_36755
6Dasn
 15 76,4% 68 4.991E-32
4 phalp2_16154
4tLR7
 1 49,3% 81 3.869E-22
5 phalp2_33510
39GU
 2 37,5% 96 2.580E-21
6 phalp2_9052
5m3bR
 2 31,3% 86 1.049E-18

Domains

Domains [InterPro]
Unannotated
Representative sequence (used for alignment): 8jDjT (111 AA)
Member sequence: U5P0A9 (158 AA)
1 111 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated

Taxonomy

  Name Taxonomy ID Lineage
Phage Shigella phage SfIV
[NCBI] 		1407493 No lineage information
Host Shigella flexneri
[NCBI] 		623 Proteobacteria > Gammaproteobacteria > Enterobacteriales > Enterobacteriaceae > Shigella >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KC814930 [NCBI]
CDS location
range 38046 -> 38522
strand +
CDS
ATGCAGGTACTAAATTCCCAGCGTATAGCTTTCCTCGATATGGTGGCATGGTCAGAAGGAACGGATAACGGGCGACAACCGACACGTAACCACGGTTATGATGTTATTGTTGGCGGCGAACTGTTTACTGATTACTCTGATCACCCTCGCAAACTTGTCACGCTAAACCCAAAACTCAAATCAACAGCCGCCGGACGTTACCAGCTTCTTTCCCGTTGGTGGGATGCCTATCGCAAGCAGCTTGGCCTGAAAGACTTCTCTCCGAAAAGCCAGGACGCTGTGGCATTGCAGCAGATTAAAGAGCGTGGCGCTTTACCGATGATTGATCGCGGCGATATTCGTCAGGCTATCGATCGTTGCAGCAATATCTGGGCGTCGTTACCTGGTGCAGGTTACGGTCAGTATGAACATAAAATCGGTGACCTGATTTCCCGGTTTAAAGAGGCTGGTGGGGTGGTAAATGAAGTTGAGCTATAA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016829 lyase activity molecular function None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
4.2.2.n2 None Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		HAMAP-Rule:MF_04109

Tertiary structure

PDB ID
upi00026e3c68_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8jDjT) rather than this protein.
PDB ID
8jDjT
Method AlphaFoldv2
Resolution 68.24
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50