Protein

Protein accession
D7RWK6 [UniProt]
Representative
7skiR
Source
UniProt (cluster: phalp2_10054)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVKVINKSVCRGVAGKRVGNVKGVVIHNDAGAVGATAESYVKRLESMTNKQLENGFAHYYIDRNTVARVEDTYNKAWHTANQDGNAYYIGYEVCQSLGASDKEFLANEQATFKQVAEDLKFYGLKANRDTVRLHREFVATACPHRSWELHGKSINSVKDYFIAQINKYMGVTTPKPPAAVKPPAKPKPPVKPQGIKRVAEKGTFYPNTTVAIKHTPTIKAKQEATLGKGESVIYDSYVQSDGYVWVSYIRNNGKRGYACSRDIKTGKAYGEFK
Physico‐chemical
properties
protein length:273 AA
molecular weight:30351,1 Da
isoelectric point:9,59
hydropathy:-0,57
Representative Protein Details
Accession
7skiR
Protein name
7skiR
Sequence length
482 AA
Molecular weight
54856,31980 Da
Isoelectric point
8,02556
Sequence
MDKNIERIIKVYRATDTPFFLSVVIAQYMIESNNGTSELAKQAHNYFGHKIGENWNGQIYYKDATEEQNGKMVNVGQTAWRKYSTLEECVADHLNWLTQTEWYKNYYADAINAKTYKEQAEVLEGKYATASYYAEAIIDKIEKLKLYEYDQEKGSDQVMGIKKPKRLFADYLHAGYMNPIKGVVIHNDAGSIGGTATFYKGWLRNRDKDLGIAHYYITEEEIGRYINTNEVGWHTANTEGNGHYIGYEVCQSIGASREEFLRNEDMTLRQVAEDMLFYKIPVNRNTVRLHKEFSSTTCPHRSWDLHGQSVNSVKDYWIAKIKHYQSLGKTVDEMIDAENKGVKAPVSKPQAPKSEPKPTIKKAENIFAELKEGQEVTIRKQMTHWFIPDGNGGKKPSISVAGKKDKIKKVISCDKSNSKRAYLLAGFNSWILEQDLEEAYASLTKVEGAPAKQNPTPAVAMVDGEGYVHLNGVKYKVNLTKE
Other Proteins in cluster: phalp2_10054
Total (incl. this protein): 4 Avg length: 319,8 Avg pI: 9,18
Protein ID Length (AA) pI
7skiR 482 8,02556
D7RWE7 251 9,43529
A0AAX4G403 273 9,68168
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_11372
7BXB0
 3 43,2% 347 3.528E-74
2 phalp2_7784
7lEhL
 1 38,6% 321 4.719E-52
3 phalp2_32385
yZyv
 16 33,4% 377 2.262E-36
4 phalp2_3379
3pnB5
 146 27,2% 338 3.369E-20
5 phalp2_9614
1Eh3E
 1 26,9% 546 5.953E-20
6 phalp2_24803
725t1
 19 28,0% 435 1.798E-18

Domains

Domains [InterPro]
GLUCO
Ami2
Unannotated
Representative sequence (used for alignment): 7skiR (482 AA)
Member sequence: D7RWK6 (273 AA)
1 482 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Brochothrix phage BL3
[NCBI] 		764562 No lineage information
Host Brochothrix thermosphacta
[NCBI] 		2756 Firmicutes > Bacilli > Bacillales > Listeriaceae > Brochothrix >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
HM144386 [NCBI]
CDS location
range 21004 -> 21825
strand +
CDS
ATGGTAAAAGTAATTAACAAATCAGTATGTAGAGGTGTCGCAGGTAAACGTGTAGGCAATGTGAAAGGTGTTGTTATTCATAATGATGCGGGTGCTGTTGGAGCAACTGCTGAATCATACGTTAAACGTTTAGAATCAATGACGAACAAGCAGTTGGAAAACGGTTTCGCTCACTACTACATTGACCGCAACACAGTCGCACGTGTGGAAGATACATACAACAAAGCATGGCATACAGCTAATCAAGATGGTAATGCGTATTACATCGGTTACGAAGTGTGTCAGTCACTAGGTGCAAGTGATAAAGAGTTTCTTGCGAACGAACAAGCGACATTTAAACAAGTAGCAGAGGATCTTAAATTTTATGGTTTGAAAGCTAATCGAGATACTGTACGCCTGCATCGTGAGTTTGTAGCAACTGCATGTCCACATCGTTCTTGGGAATTGCATGGCAAGTCAATTAACAGCGTTAAGGACTACTTTATCGCTCAGATTAATAAATATATGGGTGTGACAACACCTAAACCACCTGCAGCAGTTAAACCGCCTGCAAAACCTAAGCCGCCTGTTAAACCGCAAGGAATTAAGCGTGTGGCTGAAAAAGGCACGTTCTATCCTAATACAACTGTTGCTATCAAACATACGCCCACAATCAAAGCTAAGCAAGAAGCTACGCTAGGCAAAGGCGAGTCCGTTATCTATGATAGCTATGTTCAGTCAGATGGCTATGTTTGGGTATCATATATCCGAAACAACGGTAAGCGAGGTTATGCTTGTTCTCGGGACATTAAAACAGGTAAAGCTTACGGTGAATTTAAATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
upi0001d9adc0_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7skiR) rather than this protein.
PDB ID
7skiR
Method AlphaFoldv2
Resolution 84.61
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50