Protein

Protein accession
I1TLF9 [UniProt]
Representative
7ov8y
Source
UniProt (cluster: phalp2_29974)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MDKFTVHAGHTLGGGASGSGFEESAVARQFCPVLIDALRKVGATVTDCTDNVSTTQSANLTRLINLCNAVNRDLDISLHFNSSDDPAATGVEVLYYDQRALAAKVSDAISKASGLRDRGPKERKDLAVLNGTKAPAILIELAFISNASDMKKFFDNMQAIANAIVQAVTGKAVNVDPPAPKRTATIITTGGLGQDAAKQAVDMMFAKGWYGTLTFQTDGIAVLKTGGLSGDKLQAAREYFAWRGWWYQEEVIEY
Physico‐chemical
properties
protein length:254 AA
molecular weight:27197,5 Da
isoelectric point:5,94
hydropathy:-0,11
Representative Protein Details
Accession
7ov8y
Protein name
7ov8y
Sequence length
255 AA
Molecular weight
26871,08120 Da
Isoelectric point
5,67795
Sequence
MAYVGVAGHNAIVPGADGCGYAEHAVARQMLAKVIEYMNAVGETMHNCTDDVGRTPSSCWQNAVNNTNRAIGSDGLAVSIHLNAANSNATGTEVLHYNNPELAAKVSAKIAGVLGIKDRGPKGGKSIGYINSTKSEAILIELCFIDNPSDMKKLMDNFDAVAAAIVEAITGKRVQSGGGNSVAVDDGKRKIQTGGLGFDAVKEVAEVMCAKNIKGSIIVPGGSESPYVLTEKIGNPYLDDFTGWLDNKGWHWDYI
Other Proteins in cluster: phalp2_29974
Total (incl. this protein): 34 Avg length: 254,0 Avg pI: 6,05
Protein ID Length (AA) pI
7ov8y 255 5,67795
1lGjL 257 7,12758
1mUFh 253 5,88468
3Llo8 257 6,44522
4E6IP 256 6,31250
71JAV 249 5,70916
7476K 252 5,51608
7A5O1 253 6,07554
7BxYP 249 5,37352
7BzfV 253 5,76395
7LSwT 257 6,44437
7QCfP 253 5,87200
7QxQg 254 6,23748
7Qy2g 251 5,70370
7ReCJ 249 5,69989
7cEQV 253 6,30637
7ea4G 253 5,39268
7et4I 253 5,70535
7h91F 253 5,88030
7hvvm 261 6,39390
7jVTb 257 6,70469
7jVhK 252 5,51392
7jeNr 253 6,59852
7mwCp 253 5,53688
7rjJS 255 5,97812
7rmm2 256 6,24163
7xv22 253 5,69995
8IFcU 253 5,69592
8IFcV 253 6,07895
8IFcW 257 6,44568
8IFd1 250 6,07401
8IFd2 259 6,82991
8Lps7 259 6,83036
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6374
7yZe6
 41 50,5% 168 6.588E-54
2 phalp2_36719
6eCmw
 993 37,2% 172 4.946E-33
3 phalp2_8747
8qMi4
 13 35,3% 198 8.674E-28
4 phalp2_15683
35AAr
 164 36,3% 176 1.394E-26
5 phalp2_2704
7cWsF
 33 33,5% 161 1.639E-25
6 phalp2_23331
5tGuH
 43 30,0% 193 7.635E-25
7 phalp2_19905
69yEp
 107 26,4% 212 6.376E-20
8 phalp2_34447
4ANo2
 39 29,0% 200 7.285E-19
9 phalp2_3696
51zXL
 234 31,3% 169 6.107E-18
10 phalp2_10528
8cmRP
 1 35,0% 171 1.517E-17

Domains

Domains [InterPro]
Ami3
Unannotated
Representative sequence (used for alignment): 7ov8y (255 AA)
Member sequence: I1TLF9 (254 AA)
1 255 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage PBC1
[NCBI] 		1161901 Pebcunavirus > Pebcunavirus PBC1
Host Bacillus cereus
[NCBI] 		1396 Firmicutes > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JQ619704 [NCBI]
CDS location
range 20824 -> 21588
strand +
CDS
ATGGATAAATTTACAGTACATGCAGGGCATACTCTTGGCGGAGGTGCTAGCGGCAGCGGCTTTGAAGAGTCAGCCGTTGCCCGTCAGTTCTGCCCAGTATTAATCGATGCTTTACGCAAAGTAGGGGCAACAGTAACGGACTGCACCGACAACGTAAGTACAACGCAAAGTGCGAACCTTACGCGTCTAATTAATCTCTGTAACGCGGTTAACCGTGACCTAGACATCTCGTTACATTTCAACTCAAGCGACGACCCTGCTGCTACGGGCGTAGAAGTCCTGTATTACGACCAAAGAGCGTTAGCGGCTAAAGTATCGGATGCAATCTCTAAAGCGTCAGGACTGCGAGACAGAGGTCCTAAAGAGCGTAAAGACCTAGCAGTATTAAACGGGACTAAAGCCCCTGCTATTCTGATTGAGTTGGCTTTCATTAGTAACGCTTCAGATATGAAGAAATTCTTTGATAACATGCAAGCTATTGCAAATGCGATTGTACAAGCCGTTACAGGCAAAGCAGTAAATGTTGATCCGCCTGCTCCTAAGCGCACCGCTACTATTATTACAACAGGCGGGTTAGGGCAGGATGCGGCTAAACAAGCAGTTGATATGATGTTCGCTAAAGGTTGGTACGGAACTCTTACCTTCCAAACAGACGGCATTGCCGTATTAAAGACTGGCGGGCTATCAGGTGATAAGTTACAAGCGGCAAGGGAATACTTTGCATGGCGCGGATGGTGGTATCAAGAAGAAGTGATTGAGTATTAA

Gene Ontology

Description Category Evidence (source)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
upi00025f1301_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7ov8y) rather than this protein.
PDB ID
7ov8y
Method AlphaFoldv2
Resolution 93.04
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50