Protein

Protein accession
F6MIJ9 [UniProt]
Representative
7BWDU
Source
UniProt (cluster: phalp2_32271)
Protein name
Putative N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTFPIRKIVIHCSATRNGKSLKQAGKSSAEVIDGWHKQRGFKRLAGAVKTFNAHLEHLGYHFVIDVDGTVETGRQVGEMGAHVKGHNLHSIGICVVGGITANGKNHAEFTEAQWLALHRLLRELEAKYPNAKIYGHRDLSPDRNGDGTIAPNEWVKDCPCFDVWEWLDSEEIVNVGHLYA
Physico‐chemical
properties
protein length:180 AA
molecular weight:20031,5 Da
isoelectric point:7,20
hydropathy:-0,37
Representative Protein Details
Accession
7BWDU
Protein name
7BWDU
Sequence length
133 AA
Molecular weight
14891,49680 Da
Isoelectric point
8,73632
Sequence
MHTSPITKIVIHCSATQNGKQLRTTTQTAAQRIDDWHKQRVFRCLADNYKQFNPHLQHIGYHFVIDTDGTVETGSKEGETGAHVKGHNLNSLGICLVGGITKDKRNHSEYTEAQSLASPIRSQISQCSYLWTS
Other Proteins in cluster: phalp2_32271
Total (incl. this protein): 12 Avg length: 167,4 Avg pI: 7,71
Protein ID Length (AA) pI
7BWDU 133 8,73632
73nKo 181 7,19646
7qIcE 177 6,88436
7r4z4 124 9,00412
7tXUD 148 9,40447
7wUhA 182 8,28633
8auQC 179 6,40913
MQjw 182 7,19595
A0A2Z4PZH7 178 6,66212
A0A8S5L7Y2 164 7,79650
A0A8S5U3S8 181 7,72950
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3887
6RreM
 7260 41,5% 113 4.841E-28
2 phalp2_11894
2jrH4
 329 39,6% 111 7.768E-23
3 phalp2_27860
8AZLJ
 3 40,2% 97 5.149E-22
4 phalp2_39718
8z0Eo
 35 40,9% 83 9.894E-19
5 phalp2_21606
2vySa
 303 38,0% 100 1.682E-17
6 phalp2_24952
kjdf
 441 34,1% 120 4.357E-14
7 phalp2_20530
4iTZt
 5 32,2% 152 1.005E-12
8 phalp2_9904
2GCXD
 1 41,1% 85 1.375E-12
9 phalp2_11611
1k5JO
 2203 32,4% 111 3.158E-11
10 phalp2_25923
64Nde
 24 30,7% 91 2.823E-10

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 7BWDU (133 AA)
Member sequence: F6MIJ9 (180 AA)
1 133 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Haemophilus phage SuMu
[NCBI] 		483266 No lineage information
Host [Haemophilus] parasuis
[NCBI] 		738 Proteobacteria > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Haemophilus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JF832915 [NCBI]
CDS location
range 10121 -> 10663
strand +
CDS
ATGACCTTTCCTATTAGAAAAATCGTAATTCATTGTTCTGCCACTCGCAACGGCAAAAGTTTAAAGCAAGCAGGAAAATCATCAGCAGAGGTGATTGATGGCTGGCATAAGCAACGTGGCTTTAAGCGATTGGCAGGGGCGGTGAAAACCTTTAATGCTCACCTTGAACACCTTGGTTATCACTTTGTGATTGATGTGGACGGAACTGTTGAAACGGGTCGTCAGGTTGGTGAAATGGGGGCGCACGTCAAGGGGCACAATCTCCACTCTATCGGGATTTGTGTGGTGGGCGGTATTACGGCTAATGGTAAAAATCACGCTGAATTTACTGAAGCACAATGGCTCGCCTTGCACCGTTTACTGCGTGAGCTTGAAGCGAAATACCCGAATGCGAAGATTTATGGGCATCGGGATTTAAGTCCTGATCGTAATGGTGATGGCACTATTGCCCCGAATGAGTGGGTGAAAGATTGCCCCTGCTTTGATGTGTGGGAATGGTTAGATAGTGAAGAAATTGTGAATGTGGGGCATTTATATGCGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008270 zinc ion binding molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
upi0002118fd0_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7BWDU) rather than this protein.
PDB ID
7BWDU
Method AlphaFoldv2
Resolution 81.18
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50