Protein

Protein accession
F8UBI4 [UniProt]
Representative
7z5TK
Source
UniProt (cluster: phalp2_27800)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKIALRGGHSPNCKGANVLRDEQSCMWALADEVEKVLTSHGHTVVRCETTLSNEREDVRQGAKKGYNCDMFISLHMNASDGRGNGTEAWVARSARSSIKEIASRLCKNYATLGLQNRGVKEKNYWEMTDTNCPNIIFETMFCDDKHDIDIWASTSWDKLARLIANAIDPNIPLEKEQDYYRVAVQRFKSKEDAEKAKQRISSELGYYCFTEKI
Physico‐chemical
properties
protein length:213 AA
molecular weight:24253,0 Da
isoelectric point:6,95
hydropathy:-0,64
Representative Protein Details
Accession
7z5TK
Protein name
7z5TK
Sequence length
300 AA
Molecular weight
32944,98850 Da
Isoelectric point
5,91384
Sequence
MKIAFRGGHNEQATGARGIIDELTEDRRVLAECINVAKEQGHEVLDCTPGPCDVNTDLVAGVNKANAWGADIYIPIHFDKAYSYYEGAIGTGTFVYSKNDSIKDEDIAQRISNNIAVLGFKNRGVKEADYYDLRMTKMQAVLVEVCFCEATEDVALYRKLGPRKIAEAIVGGILNKNISGTNNQNNIEKVEYKMETIVLYTNPIDECGAKFLAYAVGGMAYDAKVPFNFDGGKVAKRIIAVGGDNPRKGEAGQFGFTGYITHKIADGDRFDTLNVCNDIAKRVSKGENIDNLLSKYKINK
Other Proteins in cluster: phalp2_27800
Total (incl. this protein): 19 Avg length: 277,0 Avg pI: 7,02
Protein ID Length (AA) pI
7z5TK 300 5,91384
3Parl 300 7,65902
3wn0r 302 7,58399
3xW7g 314 5,65374
5Lvzn 299 8,14888
5OLA9 302 8,16216
5YPhR 310 6,83514
60FNS 302 7,58399
67coi 225 8,81749
7pwHS 308 6,83514
7z5Tc 300 5,91673
82qI1 289 9,46945
85WnO 289 9,46945
8pyIe 314 6,38691
A0A2D0WXW5 213 5,46145
B6CXF7 213 6,23185
A0A1L2BYB1 257 4,98418
A0A8E6LV45 213 5,31794
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_35186
67E6C
 93 53,0% 198 6.380E-84
2 phalp2_36719
6eCmw
 993 48,1% 189 1.159E-57
3 phalp2_19930
6wdij
 24 34,4% 183 8.765E-43
4 phalp2_22023
5iret
 1 36,4% 192 1.542E-37
5 phalp2_2703
7cyBj
 192 37,4% 187 1.542E-37
6 phalp2_8475
1agfv
 1 31,6% 202 2.538E-34
7 phalp2_14956
ooF5
 39 31,7% 205 6.395E-34
8 phalp2_6374
7yZe6
 41 33,5% 197 1.390E-30
9 phalp2_10022
75Uho
 6 28,1% 192 2.149E-27
10 phalp2_33519
6ass
 2 34,4% 218 4.533E-26

Domains

Domains [InterPro]
Ami3
Unannotated
Representative sequence (used for alignment): 7z5TK (300 AA)
Member sequence: F8UBI4 (213 AA)
1 300 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520

Taxonomy

  Name Taxonomy ID Lineage
Phage Clostridium phage phiCP34O
[NCBI] 		1042123 No lineage information
Host Clostridium perfringens
[NCBI] 		1502 Firmicutes > Clostridia > Clostridiales > Clostridiaceae > Clostridium >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
JF767209 [NCBI]
CDS location
range 24442 -> 25083
strand +
CDS
ATGAAAATAGCTTTAAGAGGTGGACATTCACCTAATTGCAAAGGTGCTAACGTTTTAAGAGATGAGCAATCTTGCATGTGGGCTTTAGCTGATGAAGTAGAAAAAGTTTTAACCTCTCATGGTCATACCGTTGTAAGATGTGAAACAACTTTATCAAATGAAAGAGAAGATGTAAGACAAGGGGCTAAAAAAGGTTATAATTGCGATATGTTTATCTCTCTTCACATGAATGCAAGTGACGGTCGAGGAAATGGCACGGAAGCGTGGGTTGCTAGAAGTGCGAGAAGTTCTATAAAAGAAATTGCTTCAAGATTATGCAAAAACTATGCAACTTTAGGATTGCAAAACAGAGGTGTAAAAGAGAAGAATTACTGGGAAATGACAGATACAAATTGCCCTAATATTATTTTTGAAACTATGTTCTGTGATGATAAGCATGATATAGATATATGGGCTTCAACCTCTTGGGATAAATTAGCGAGATTAATCGCAAATGCTATTGACCCTAACATTCCACTAGAAAAGGAACAAGACTATTATAGAGTTGCAGTTCAGAGATTTAAAAGTAAAGAAGATGCAGAAAAAGCGAAACAAAGAATAAGCAGTGAATTAGGTTATTATTGCTTTACTGAAAAGATATAG

Gene Ontology

Description Category Evidence (source)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042834 peptidoglycan binding molecular function None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
upi000214c757_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7z5TK) rather than this protein.
PDB ID
7z5TK
Method AlphaFoldv2
Resolution 90.84
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50