Protein

Protein accession
D4N4K8 [UniProt]
Representative
7vQh7
Source
UniProt (cluster: phalp2_28103)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTKNINGDLYSDLITSADSRAMASGSIPRKTIDRIVIHHNATTNKDVAINTWLASGSAQTSAHYEVANNEIIGIVGEGTTAWHAGNGDMNARSIGIENLNSAGEPNWPVSSQTFESLSKLVADIAKRYGFPIDSTHVIPHNAVVGTRCPGGIDVAKVIARAREIAGGNGGSNINTGNNTNELDQVLHVGEYFKARKAYRVDEMKFVNGVWQVVNYELAGGKDFSWVYNGFGVASTDKVDSNGNITKDQELSVGAYFRLHSDRIKVVDANDSGIALDTRYGRVWVDASTLTEVK
Physico‐chemical
properties
protein length:293 AA
molecular weight:31601,7 Da
isoelectric point:5,90
hydropathy:-0,31
Representative Protein Details
Accession
7vQh7
Protein name
7vQh7
Sequence length
315 AA
Molecular weight
33362,05620 Da
Isoelectric point
9,14389
Sequence
MTFSKLTTYVDTRPMNGSPANSATYPRHKIDRIVIHHNSGTNYKGALDTWLVGGPANTSAHYEITPTMIIGCVEEERSAWHAGNKLMNQRSIGLEHVNSAGAPGWPVAEETLKNSAKLCADLCDRYDIPIDATHIVPHNSVVPTDCPGGINMSHYIDLVRQAAGQPATTPTPTPAPSGKTITQLAAEVIAGKYGSGDARKAALGSQYAAVQAEVNRQLGGTASAPKPAPAPSNWHAQTGSFTITTSPGIKLRSGSASTSASLLAVLPKGSVVKYDAYGYFDGYVWIRQPRSGGYGYLPTGTASGTKRTSYWGSFK
Other Proteins in cluster: phalp2_28103
Total (incl. this protein): 9 Avg length: 286,2 Avg pI: 7,50
Protein ID Length (AA) pI
7vQh7 315 9,14389
7vG7k 278 9,69645
7vG9M 277 9,66350
7vQEI 335 6,29335
8nXIq 199 8,52261
A0A0D3MKG1 293 6,23793
A0A059PAL4 293 6,06167
A0A0D3MK08 293 5,97846
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_18388
5HE4u
 2 72,0% 200 5.038E-82
2 phalp2_18952
3MmP0
 1 40,4% 341 1.546E-74
3 phalp2_20487
3X7ua
 81 42,0% 250 7.266E-45
4 phalp2_1607
7ZyR0
 345 43,4% 223 2.511E-44
5 phalp2_6225
6oM64
 106 37,6% 210 2.785E-30
6 phalp2_14834
7s5S0
 47 41,4% 217 7.671E-27
7 phalp2_7641
690y3
 22 28,6% 248 1.103E-20
8 phalp2_30585
5Zqsx
 178 28,8% 291 8.946E-20
9 phalp2_13871
7vJcf
 1 25,9% 347 9.711E-19
10 phalp2_22264
7pxLN
 64 23,6% 326 1.407E-17

Domains

Domains [InterPro]
Ami2
CW_7
SH3_5
Representative sequence (used for alignment): 7vQh7 (315 AA)
Member sequence: D4N4K8 (293 AA)
1 315 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08230, PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Leuconostoc phage 1-A4
[NCBI] 		745088 Unaquatrovirus > Unaquatrovirus uv1A4
Host Leuconostoc mesenteroides
[NCBI] 		1245 Firmicutes > Bacilli > Lactobacillales > Leuconostocaceae > Leuconostoc >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
GQ451696 [NCBI]
CDS location
range 23473 -> 24354
strand -
CDS
ATGACAAAGAATATTAATGGTGATTTGTATAGTGACCTGATTACCAGTGCAGATAGTCGAGCAATGGCGAGTGGAAGTATCCCTCGTAAGACTATTGATCGTATCGTTATCCACCACAACGCAACAACAAATAAAGATGTAGCAATCAATACGTGGTTGGCTTCTGGATCTGCACAAACATCGGCTCATTATGAAGTGGCTAACAACGAAATTATTGGTATTGTTGGTGAGGGGACAACCGCATGGCACGCTGGTAACGGAGACATGAACGCTCGCTCGATTGGTATTGAAAACCTTAACAGTGCGGGAGAACCTAACTGGCCGGTGTCATCACAAACGTTCGAAAGTCTATCTAAATTAGTAGCTGACATTGCAAAGCGTTATGGTTTCCCTATCGATAGTACACACGTTATCCCTCATAACGCGGTTGTTGGTACTCGTTGCCCCGGTGGTATTGATGTGGCTAAGGTTATCGCTCGCGCTCGTGAAATTGCTGGTGGTAATGGTGGCTCAAATATTAACACTGGAAACAACACAAATGAATTAGACCAAGTGTTGCACGTGGGAGAATATTTCAAAGCACGTAAAGCATATCGAGTTGACGAAATGAAGTTTGTTAACGGCGTGTGGCAAGTGGTAAACTACGAACTAGCCGGTGGTAAAGACTTCTCGTGGGTATATAATGGGTTTGGTGTTGCCTCTACTGATAAGGTTGACTCCAATGGTAATATCACAAAAGACCAAGAGTTATCCGTTGGCGCTTACTTCCGACTACACAGCGATCGTATTAAGGTTGTTGACGCAAATGACAGTGGTATTGCATTAGATACTCGTTATGGTCGTGTTTGGGTAGACGCTTCAACATTAACAGAGGTAAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001cd9152_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7vQh7) rather than this protein.
PDB ID
7vQh7
Method AlphaFoldv2
Resolution 86.75
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50