Protein

Protein accession
N0DSE8 [UniProt]
Representative
2SpyY
Source
UniProt (cluster: phalp2_6942)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MQKRNKLAGGLLALAMGIAAGYEGYRTIAYPDVGNVWTICYGETAGVKRGDTANKQQCDSMLVRSLLKHNDHLTALPQMPPNVHLAVLDWTYNVGTGNARGSTLWKYLQQGEWEKACGQFTRWRFAAGKDCAKDKSCRGVYLRRVDEAALCRGEITPDQLIAKLGSSLGDPDGATAQ
Physico‐chemical
properties
protein length:177 AA
molecular weight:19336,0 Da
isoelectric point:8,90
hydropathy:-0,39
Representative Protein Details
Accession
2SpyY
Protein name
2SpyY
Sequence length
80 AA
Molecular weight
8649,68260 Da
Isoelectric point
7,99442
Sequence
VTGAKRVSLNTRVATMFGAISMLIAGVMATQQEGTRYVAYQDVGGTWTICEGDTHNVKQGDTATREQCDDRLRKNLIAAS
Other Proteins in cluster: phalp2_6942
Total (incl. this protein): 4 Avg length: 153,0 Avg pI: 8,72
Protein ID Length (AA) pI
2SpyY 80 7,99442
L0MZ33 177 8,91625
A0A4Y5TWP4 178 9,06904
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_24269
3bvr0
 10 39,2% 51 3.122E-09
2 phalp2_30282
4kWNQ
 1 39,3% 61 1.532E-08
3 phalp2_17749
7tNIh
 28 36,6% 71 7.519E-08
4 phalp2_6237
6y3FI
 8 38,8% 54 2.492E-06
5 phalp2_20985
7xCJE
 9 33,3% 63 3.426E-06

Domains

Domains [InterPro]
Disordered region
GH24
Representative sequence (used for alignment): 2SpyY (80 AA)
Member sequence: N0DSE8 (177 AA)
1 80 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF00959

Taxonomy

  Name Taxonomy ID Lineage
Phage Edwardsiella phage PEi21
[NCBI] 		1325372 Yokohamavirus > Yokohamavirus PEi21
Host Edwardsiella ictaluri
[NCBI] 		67780 Proteobacteria > Gammaproteobacteria > Enterobacteriales > Enterobacteriaceae > Edwardsiella >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AP013057 [NCBI]
CDS location
range 27054 -> 27587
strand -
CDS
ATGCAAAAACGCAACAAACTGGCCGGCGGACTACTCGCGCTGGCAATGGGTATCGCTGCCGGTTATGAGGGGTACCGGACAATCGCTTACCCGGACGTGGGCAACGTGTGGACGATTTGCTACGGTGAGACGGCTGGTGTCAAGCGTGGTGACACAGCCAACAAGCAACAGTGTGACAGTATGTTGGTGCGCTCACTCTTGAAACATAACGACCATCTCACAGCTTTGCCGCAAATGCCGCCTAACGTTCACCTGGCTGTACTTGACTGGACGTACAATGTCGGCACGGGCAACGCACGCGGCTCCACACTGTGGAAATACTTACAGCAAGGCGAGTGGGAAAAAGCGTGTGGACAATTCACACGCTGGCGGTTTGCAGCAGGTAAAGACTGTGCGAAAGACAAATCGTGCAGGGGCGTTTACCTTCGTCGTGTTGATGAAGCCGCACTATGTCGTGGTGAAATAACCCCTGACCAACTCATCGCCAAGTTAGGTTCCTCGCTTGGCGACCCGGACGGAGCAACTGCACAATGA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

PDB ID
A0AAE7VK59
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
N0DSE8
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi00032722ec_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (2SpyY) rather than this protein.
PDB ID
2SpyY
Method AlphaFoldv2
Resolution 75.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50