Protein

Protein accession
A0A4D6B353 [UniProt]
Representative
7ewxf
Source
UniProt (cluster: phalp2_26061)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MVAYLDFKSRVLNNGYDHDGGAFGWQCWDGFAELCVANGIPIINTDPINHSGYAKDLWEQRKTNGMLTYFDEVDTMEGGDVAIFKEVATATPSSHVAIFDSDIDGVYGWFLGQNQGGIPNPNGGSAFNLVMLPYSATYPTAFRVKKGAKMQVKNVNGDIYSNLITSSRPEIFGSWGERDIKSIKYIVLHGTYGLSVQSAVDAWTGGREASAQYIVHDNQIVGCVGENFTAWHCGGTGAITNQNSIGVEHVNNKINGENSTFSAQTIETGAKLVAEICKRLGIVPSNKTIVPHKSVFATACPQAMDVPAYIEKVKQYYNETPKKETPKPQTKETKSVASNEQTSARLLVFDEDLNGYKKGDYILLNFDRGTYSKVSNKAEVDFIKKQYTGIQTEHVSKGYPAHVRYIQGFKLNKI
Physico‐chemical
properties
protein length:414 AA
molecular weight:45582,0 Da
isoelectric point:6,56
hydropathy:-0,37
Representative Protein Details
Accession
7ewxf
Protein name
7ewxf
Sequence length
420 AA
Molecular weight
46605,74680 Da
Isoelectric point
6,35502
Sequence
MVSYLDFKSRVLNHGFDHDGGAYGWQCWDGFAEWCVANGIPIINTDPVHHTGYAMDLWELRQSNGMLTYFDEVETMEGGDVAVFKEVTGVTPSSHVAIFDSDIDGTYGWFLGQNQGSTITHPSGGSAFNLVMLPYSATYPTAFRIKKKVEAAKSMGTKNVNGDIYSDLITSSRPELFGNWGVRDMKSIKYIVIHGTLGLSVQSAVNAWTGGRQASANYIVHDNQIVGCVGENYTAWHCGGQGAITNQNSIGIEHVNATLNGANSTFSAQTVETGAKLVAEICKRLGIVPSRKTIVGHKEVYATACPQSLNLDDYVKKVLSYYNGKTSSQTKKETPKTAPNQPNKQPSARIIVFDEDIDNFKKDQNWLFNFDRGTYNYIANTEELKFIKKQYPDIQTEHVSKKYPAHIRYIEGFNLVNLDK
Other Proteins in cluster: phalp2_26061
Total (incl. this protein): 55 Avg length: 433,5 Avg pI: 6,76
Protein ID Length (AA) pI
7ewxf 420 6,35502
1HJAt 447 6,86242
1cwPe 444 6,88737
2aM9T 445 6,86038
3vYLE 444 7,17305
5RUx7 446 6,31927
5XBP2 446 6,63808
5Xr6j 446 6,50007
61Acr 445 7,17305
65yBj 445 6,86094
66hf7 453 7,60684
68cxP 446 6,39401
69caK 447 7,17867
69xxJ 445 7,17248
6Zzx8 446 6,67070
6aBcT 446 6,49905
6dp9l 446 6,86055
6fgad 446 6,46273
6hStY 446 6,67178
705dw 444 6,88726
71GOx 440 6,39390
73iZP 418 6,37662
73j24 415 6,37469
7C2cF 415 6,64126
7C68B 414 6,56129
7MgH4 371 7,15196
7NGiR 370 8,32127
7O1Sm 446 6,53355
7Xqfc 446 6,67201
7YXoJ 446 6,53304
7ZkwZ 426 6,89101
86ah8 446 6,49899
877Sv 436 6,22656
8ci0n 446 6,23140
8hhS6 445 6,85998
8lEFs 446 6,31233
8qTxP 446 6,88760
8sDI5 445 7,17305
8sXnz 446 6,49632
BHBH 445 7,17305
N47Q 446 6,31421
NHT6 444 6,66888
NqQd 444 6,70060
NqS6 446 6,30728
p4v5 446 6,46137
qImc 365 6,66582
zlTO 370 8,50991
A0A4D6ALQ3 420 6,35502
A0A4D6A9N0 418 6,37662
A0A4D6B1N0 415 6,37469
A0A4D6AC33 415 6,64126
A0A0B5A075 439 7,10529
A0A4D6AAN8 429 7,12866
A0A4D6B3L2 429 7,12866
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_11189
5Xg2q
 31 44,6% 271 7.808E-79

Domains

Domains [InterPro]
Unannotated
Ami2
Unannotated
Representative sequence (used for alignment): 7ewxf (420 AA)
Member sequence: A0A4D6B353 (414 AA)
1 420 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptococcus phage Javan400
[NCBI] 		2548150 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK448929 [NCBI]
CDS location
range 35317 -> 36561
strand +
CDS
ATGGTTGCATACCTTGATTTTAAAAGCAGAGTTTTAAATAATGGTTATGATCATGACGGAGGAGCTTTTGGCTGGCAGTGTTGGGACGGTTTCGCAGAATTGTGCGTGGCAAATGGCATTCCAATCATTAACACTGACCCTATAAATCATAGTGGATATGCAAAAGACTTATGGGAGCAACGTAAAACAAATGGCATGCTAACCTATTTCGATGAGGTCGATACCATGGAAGGTGGTGATGTTGCAATCTTTAAAGAGGTTGCAACTGCTACACCATCTAGCCATGTAGCTATTTTTGATAGTGACATTGACGGCGTTTATGGATGGTTCCTTGGTCAAAATCAAGGCGGTATCCCAAATCCAAACGGTGGCTCAGCGTTTAATCTTGTCATGTTGCCTTATTCAGCAACGTATCCAACAGCATTTAGAGTTAAAAAAGGAGCGAAAATGCAAGTTAAAAACGTAAATGGAGATATTTACTCAAACTTAATCACTAGCTCACGTCCTGAAATCTTTGGAAGTTGGGGAGAACGTGACATAAAATCTATTAAATATATTGTATTGCATGGCACTTATGGCTTATCAGTACAAAGTGCAGTTGATGCTTGGACTGGCGGAAGAGAAGCAAGCGCACAGTACATTGTACACGATAATCAAATTGTTGGTTGTGTTGGCGAAAACTTCACTGCTTGGCATTGTGGAGGAACAGGCGCAATCACTAACCAAAATAGCATTGGTGTAGAGCATGTAAACAATAAAATTAATGGAGAAAATTCCACTTTCTCAGCTCAAACAATTGAGACTGGAGCTAAGCTAGTAGCTGAAATTTGTAAGCGCCTAGGAATTGTACCAAGCAATAAAACTATCGTACCTCATAAGTCGGTTTTTGCAACCGCATGCCCCCAAGCTATGGACGTACCAGCGTATATCGAAAAAGTTAAACAGTATTACAATGAAACGCCTAAAAAAGAAACACCTAAACCACAAACAAAGGAGACCAAATCAGTGGCATCCAATGAACAAACTAGCGCAAGACTACTTGTATTTGATGAAGATCTAAACGGATATAAAAAAGGCGATTACATATTACTAAATTTTGACCGTGGAACTTATAGCAAAGTAAGTAATAAAGCAGAAGTTGACTTTATCAAAAAACAATACACTGGAATCCAAACAGAACATGTAAGTAAAGGATATCCTGCTCACGTAAGATATATCCAAGGATTCAAACTTAATAAGATATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi00020322ae_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7ewxf) rather than this protein.
PDB ID
7ewxf
Method AlphaFoldv2
Resolution 86.84
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50