Protein

Protein accession
Q332B8 [UniProt]
Representative
7BmmE
Source
UniProt (cluster: phalp2_429)
Protein name
Putative N-acetylmuramoyl-L-alanine amidase
Lysin probability
99%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSKKTDSFINKIKPSAIETQEKYRIFASITISQAILESGWGCSDLAKKYNNLFGIKALRDWSGQVIHIQTKEWTKNEIVTIKQPFRVYKNWSESIEDHAKFLNKEWYIDAGVFNAKDYKAQINAIVKGGYTSDPNYISKILDLINKYHLTQYDKLACDNAMIEQSEIKNITNRETKVINNEVYGIVTASVLNIRDGASTNSIKIGKLIKGEQVHIFKDYGNWLSIYYGDHGGYISSKYVEMI
Physico‐chemical
properties
protein length:242 AA
molecular weight:27730,3 Da
isoelectric point:8,91
hydropathy:-0,42
Representative Protein Details
Accession
7BmmE
Protein name
7BmmE
Sequence length
426 AA
Molecular weight
47792,84880 Da
Isoelectric point
9,20623
Sequence
MSKQTEFIEKIKDAAIEVQSQYSIFASISIAQAILESGWGESELAKNYNNLFGIKALRDWTGETVVLDTREWTQDGIVTVNQPFRVYKSWRESILDHALFLKKEWYIEAGVFKATNPTMQIQAIFDGGYCTDPKYSIKILKLINDYNLEKYDEIKDNKSKKGSSNMKIAVRGGHNFQATGASGIISETVEDRKVKDAVIKYLKQRGIQVLDVTPGKMSKEKDLAYGINKAKEWGADMFFSIHFNNAYSKYSGAIGTEVILKYGSPMLTTAKRVNSKLVNLGFKRHGNGIVYNEHLYELNHFNSAMIIEVCFVESTKDVATYKGIGVDAIGKSIVEGIFDIKINSSYTSNNSNNQKYNPGSLDGRMAICTGNGVRVRSSMDTSNTSNVIGKLNKGDKIKIFKRVGNWYHTYYGQHGGYVSAKYLNLI
Other Proteins in cluster: phalp2_429
Total (incl. this protein): 20 Avg length: 395,0 Avg pI: 8,43
Protein ID Length (AA) pI
7BmmE 426 9,20623
6J7G1 439 5,23290
7cBY1 381 8,84076
7gwtc 407 6,97422
7qqL9 380 9,23808
7qqMJ 383 8,96351
7qy2f 382 9,28321
7qy3z 411 7,14957
7qy47 383 9,08097
7rrAA 431 8,78370
7rrEI 431 9,00051
7rrhz 379 8,65038
7rrja 383 8,67301
7rrkg 431 9,00664
7rrlI 381 9,22215
7uLHx 412 7,02129
7uLIC 409 8,15269
7uLTk 384 8,03781
7wN7T 424 9,13822
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_37594
45uQn
 106 35,6% 441 3.113E-75
2 phalp2_24803
725t1
 19 31,7% 331 5.017E-48
3 phalp2_20406
39Yjh
 13 31,1% 324 1.591E-43
4 phalp2_2700
7bONi
 21 30,5% 350 3.246E-37
5 phalp2_37214
234pK
 1 28,6% 335 1.199E-35
6 phalp2_11791
82p55
 1 25,7% 342 3.197E-26

Domains

Domains [InterPro]
GLUCO
Ami3
SH3_3
Representative sequence (used for alignment): 7BmmE (426 AA)
Member sequence: Q332B8 (242 AA)
1 426 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520, PF01832, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Clostridium botulinum C phage (Clostridium botulinum C bacteriophage)
[NCBI] 		12336 No lineage information
Host Clostridium botulinum
[NCBI] 		1491 Firmicutes > Clostridia > Clostridiales > Clostridiaceae > Clostridium >
Host Clostridium botulinum C
[NCBI] 		36828 Firmicutes > Clostridia > Clostridiales > Clostridiaceae > Clostridium >
Host Clostridium botulinum C str. Stockholm
[NCBI] 		929505 Firmicutes > Clostridia > Clostridiales > Clostridiaceae > Clostridium >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AP008983 [NCBI]
CDS location
range 97772 -> 98500
strand -
CDS
ATGAGTAAAAAAACCGATAGTTTTATTAACAAAATTAAACCTAGTGCTATAGAAACTCAAGAAAAGTATCGAATATTCGCTAGTATAACCATATCACAAGCAATATTAGAATCAGGATGGGGTTGTAGTGACTTAGCTAAGAAGTATAACAATTTATTTGGAATTAAAGCATTGAGAGATTGGAGTGGACAAGTCATCCATATTCAAACAAAAGAATGGACTAAAAACGAAATTGTTACAATAAAACAACCTTTTAGAGTTTATAAAAATTGGTCAGAAAGTATAGAAGACCATGCAAAATTTTTAAACAAAGAATGGTATATAGATGCAGGAGTATTCAACGCTAAAGATTATAAAGCACAAATAAATGCCATTGTAAAAGGTGGATATACAAGCGATCCTAATTACATATCTAAAATTTTAGATTTAATAAATAAATATCACCTAACACAATATGATAAACTAGCATGCGATAATGCAATGATTGAACAAAGTGAAATCAAAAATATTACAAATAGAGAAACAAAAGTAATAAATAATGAAGTTTACGGTATAGTAACAGCTAGTGTATTAAATATAAGAGATGGTGCCAGTACTAATAGTATAAAGATTGGTAAATTAATCAAAGGTGAGCAAGTGCATATATTTAAGGATTATGGTAATTGGTTGTCTATTTATTATGGAGACCATGGAGGGTATATTAGTTCTAAATATGTAGAAATGATATAA

Gene Ontology

Description Category Evidence (source)
GO:0004040 amidase activity molecular function None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
Q332B8
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi00005db534_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7BmmE) rather than this protein.
PDB ID
7BmmE
Method AlphaFoldv2
Resolution 87.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50