Protein

Protein accession
A0A2D1GPR8 [UniProt]
Representative
eqCG
Source
UniProt (cluster: phalp2_16608)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSFVWFADEPLRTREEIAREVHKVSLARNLDELATVMCLMAIAVEVGATNHRGVYGWWCPWNEADPESQNYPHDSKSNDGRSVGYLQQQKGPRGELWWGSTQDEMTLSIAANNFLDRLSDDYVKARNNPTLAGQFVQAVQRSAYPDRYAKKWDEAWDVLRRALSEPVKQPPVSGARPSFTEHNVIGNWRGQPYGNSQSRNGREITHIVLHTSEGAGGPALLDYMKGAKVSYHYVVDNDGNAWDLVDTDRAAWSVGNANNYTINYCFGASRAGWSRQEWLNNCGNAIKITAYLVAQDCKKYNIPPVVRVGGGGNGYTSLKANKGVTDHKGINYLAGGDHWDVGPNFPWDVFNTYLQMYYGGEGDGFLMALSEQDQILLRDKVFQIWGALFNKVESQSIYRNPTPGDAGNMWATKDLIRNMDGMTHEMFVERLATLGDEDALRRVFEVASGRGAVKEDWAVNRAKEVLEKVPEEILESFLKKYDV
Physico‐chemical
properties
protein length:483 AA
molecular weight:54326,1 Da
isoelectric point:5,59
hydropathy:-0,55
Representative Protein Details
Accession
eqCG
Protein name
eqCG
Sequence length
250 AA
Molecular weight
27525,05010 Da
Isoelectric point
6,59005
Sequence
MAAPLFNEINLIPGWPNSQSRNGQKPRYAVLHTTEGAGGMELVNYMRNASVSYHYVIDNDGTVYDLVDTDEASWSCLDANNYTINYVFGASRAAWSTQQWLDKMGRAIPIAARLVAADLIKYNIPPVVSLGRPYTRINAGVIDHRYVTEVLGIGTHTDVGDGFPVDVFKQHLMAAYNELKPKAPGIPAVKPPTPPTTPVVPPKPAFSYPSQAEMVIQIWEQLFGPRGRGWPQLGGHTLVDAVAELEKRLK
Other Proteins in cluster: phalp2_16608
Total (incl. this protein): 19 Avg length: 268,9 Avg pI: 5,85
Protein ID Length (AA) pI
eqCG 250 6,59005
1fUey 289 4,89267
251zB 250 6,05588
2Znt9 252 6,28050
3dN1s 287 4,66611
46Yrg 234 5,38199
48Ywn 240 6,18126
48anl 249 7,63469
4LHtE 273 5,26331
4Lyw4 273 5,49920
4agdX 250 6,20587
4e52D 253 6,14966
75eW8 267 5,05278
7z9WJ 259 6,64467
869UA 240 6,95939
8H7U 258 5,41058
8a71Y 269 5,00890
mVlo 234 5,73178
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_23662
8MT9Q
 13 27,4% 262 8.240E-31
2 phalp2_18569
7cxQw
 45 31,4% 242 2.089E-30
3 phalp2_31178
7Vvn5
 47 32,8% 189 1.060E-23
4 phalp2_24741
6EXtT
 52 30,8% 188 7.794E-22
5 phalp2_7436
4Ksn7
 7 30,4% 164 1.954E-21
6 phalp2_22201
6VaYI
 26 30,1% 219 4.895E-21
7 phalp2_23125
4E9CJ
 94 33,3% 159 1.664E-20
8 phalp2_3964
7rht2
 43 27,7% 238 3.066E-20
9 phalp2_32230
7h6JD
 19 33,5% 155 1.915E-19
10 phalp2_10392
1NTeW
 11 23,1% 272 1.915E-19

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): eqCG (250 AA)
Member sequence: A0A2D1GPR8 (483 AA)
1 250 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Mycobacterium phage Kumao
[NCBI] 		2041344 Vilmaviridae > Kumaovirus > Kumaovirus kumao
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MG009575 [NCBI]
CDS location
range 32402 -> 33853
strand +
CDS
ATGTCTTTCGTTTGGTTTGCAGACGAGCCGCTGCGTACCCGCGAAGAGATCGCTCGCGAAGTTCACAAGGTCTCGCTCGCACGCAACCTCGACGAGCTGGCCACGGTCATGTGCCTGATGGCCATTGCCGTCGAGGTTGGTGCGACGAACCACAGGGGTGTCTACGGCTGGTGGTGCCCGTGGAACGAGGCCGATCCCGAGTCGCAGAACTACCCGCACGACTCCAAGTCGAACGACGGTCGTTCTGTCGGCTATCTGCAGCAGCAGAAGGGGCCTCGTGGCGAACTCTGGTGGGGCAGTACGCAGGACGAGATGACTCTGTCCATCGCGGCGAACAACTTCCTGGACCGCCTGTCCGACGACTACGTCAAGGCACGCAACAACCCCACGCTGGCTGGTCAGTTCGTTCAGGCCGTCCAGCGTTCGGCGTATCCAGACCGGTACGCCAAGAAGTGGGATGAGGCGTGGGATGTTCTGCGGCGGGCGCTGAGCGAGCCGGTCAAGCAGCCCCCCGTCAGCGGGGCACGGCCCTCGTTCACTGAACACAACGTGATCGGGAACTGGCGCGGCCAGCCGTACGGCAACAGCCAGTCCCGCAACGGTCGCGAGATCACCCACATCGTGCTTCACACCTCCGAGGGTGCCGGTGGTCCGGCGCTCCTGGACTACATGAAGGGCGCGAAGGTCTCGTACCACTACGTGGTCGACAACGACGGCAACGCTTGGGATCTGGTCGACACCGACCGGGCTGCCTGGTCTGTCGGCAACGCGAACAACTACACCATCAACTACTGCTTTGGTGCGTCCCGCGCCGGTTGGTCTCGCCAGGAGTGGCTGAACAACTGCGGCAACGCCATCAAGATCACCGCGTATCTGGTGGCCCAGGACTGCAAGAAGTACAACATCCCGCCCGTGGTCCGCGTCGGCGGCGGTGGCAACGGCTACACGTCACTGAAGGCCAACAAAGGCGTCACCGACCACAAGGGCATCAACTACCTGGCCGGTGGCGATCACTGGGATGTGGGTCCGAACTTCCCGTGGGACGTTTTCAACACCTACCTCCAGATGTATTACGGAGGGGAAGGAGACGGTTTTCTCATGGCACTTTCCGAGCAGGACCAGATCCTGCTGCGTGACAAGGTGTTCCAGATCTGGGGCGCTCTCTTCAACAAGGTCGAGTCGCAGTCGATCTACCGGAACCCGACTCCTGGCGACGCCGGGAACATGTGGGCCACAAAGGATCTGATTCGCAACATGGACGGCATGACCCACGAGATGTTCGTGGAGCGGCTGGCCACCCTCGGCGACGAGGACGCGTTGCGGCGGGTGTTCGAGGTGGCCTCTGGCCGGGGCGCGGTCAAGGAGGACTGGGCCGTGAACCGGGCCAAGGAGGTTCTGGAGAAGGTGCCCGAAGAGATCCTGGAGAGCTTCCTCAAGAAGTACGACGTGTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000c0c4b06_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (eqCG) rather than this protein.
PDB ID
eqCG
Method AlphaFoldv2
Resolution 89.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50