Protein

Protein accession
A0A2H5BML6 [UniProt]
Representative
5ioYm
Source
UniProt (cluster: phalp2_14594)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MARFSKAKWKPVRNYTNNGQDRVLGLIVHIMQGTLEGSQSWFDNPSAQASSHFGTGKDGELRQWVDTSDRAWAQAAGNRDYLSIENEGKVPDKLTAKQIEAVAQTFAWVAKTYKVPYQVADKPGEKGLGFHRMGGAAWGGHSCPGDNIIKQLPEIVARAKEINKVTAKPKPVYAPFPGKSFFRLGQKHKLITELGKALVKAGYKGYKVGPGQEFTRADIKAVAWFQRKQGWTGADADGYPGPETWKRLKVAQPK
Physico‐chemical
properties
protein length:254 AA
molecular weight:28070,0 Da
isoelectric point:9,86
hydropathy:-0,64
Representative Protein Details
Accession
5ioYm
Protein name
5ioYm
Sequence length
239 AA
Molecular weight
25629,35030 Da
Isoelectric point
6,97167
Sequence
MARMADASWRPIPDNYTANGQTEVRGVVVHIMAGTLSGTDSWFRNPQARASSHFGTGKAGALYQWVDTKDRAWAQADGNRTWISVENEGQGGDILTGEQLDANAEVLAWAHRVYSVPLQVASGPSGRGLGYHAMGGSAWGGHTSCPGPKIVAQLPEIVRRAKNIINAEEDSMAGMDKGDVFDASWNTDGLPAPASAPDVKKNPKWTPSSYLRSIYNGVIGIRADLKALRAAVDKLSEQK
Other Proteins in cluster: phalp2_14594
Total (incl. this protein): 94 Avg length: 277,6 Avg pI: 7,22
Protein ID Length (AA) pI
5ioYm 239 6,97167
11cco 273 8,86616
11cqb 269 8,36408
1NKgT 300 5,85978
1NKlh 300 6,07094
1cDPu 262 7,88650
1dYgW 297 5,51204
1dxeE 241 5,59002
1p2Ea 288 5,14463
1rcGS 234 9,04229
1rcm7 312 5,11019
28yw5 301 9,59897
2TrIK 276 5,24842
2UpkI 298 4,91921
2acCE 265 5,87922
2mE9Q 300 5,75349
2qPdF 304 6,55663
3M7Jd 327 5,56865
3M7zY 307 4,93780
3MLKt 294 6,54657
3NZOP 265 9,53405
3NZZE 256 6,66297
3O7Qj 265 9,06498
3O8QK 265 9,23563
3O9Qh 265 9,33478
3SsVz 232 8,39767
3Xdde 238 6,97172
3mSzW 307 5,81874
4CJ6p 246 9,16549
4DXzB 273 5,41212
4H3fe 241 8,42578
4Jl2M 294 5,53927
4JlM9 301 4,91381
4LvQz 308 5,96670
4M8Cy 276 6,32677
4MGn7 300 7,79167
4PzD5 260 4,72624
4QxwS 316 5,62708
4QyA4 311 4,77876
4QzEI 306 5,78208
4T3SW 295 7,80501
4T3zU 334 5,64271
4VmXd 305 9,23157
4W32z 304 6,53060
4YwZs 308 7,05630
5Etag 284 5,82483
5Etfh 306 5,33090
5oMyo 313 5,37603
6Akxh 247 4,99356
6DgpP 320 9,93840
6HDe0 289 10,71988
6KlA6 179 9,01598
6N8vP 290 6,05656
6Qh5Q 258 6,19257
6Qy7P 249 5,33959
6SNng 291 5,45338
6SNu3 312 5,29838
6T68B 299 5,92532
6T6Hx 267 10,53434
6T8sJ 299 5,45242
6TdCN 264 10,23502
6US7Y 259 7,09472
6Vgzs 218 5,54256
6sNi 293 8,39051
726aY 273 5,87098
7eMow 252 10,47561
7lbP0 249 5,15606
7o1Ng 307 5,20261
7ouAr 263 9,92544
7p1ZL 274 9,36927
7pYJM 273 5,79180
7plDZ 272 5,92481
7s38d 325 6,27317
7tOfu 272 10,81820
7uiZT 321 5,12889
7wfxH 336 9,02991
7zGvE 251 5,13253
7zgMz 314 9,74512
7zgp4 261 6,12175
7zhHF 248 9,99191
7zhyL 314 9,83712
7ziSm 252 10,55227
7ziob 242 6,70782
7zjYo 252 10,09145
QO76 303 5,85279
f5CX 234 9,23298
fyTt 237 8,36460
hI5v 232 5,53881
nMP9 272 5,39825
o0vM 256 9,74531
A0A2H5BMS6 253 9,85581
A0A2H5BLW9 253 9,85581
A0A3G8FP84 253 9,85581
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_2602
6IbUl
 53 43,1% 167 3.196E-59
2 phalp2_33839
1rcmi
 2 41,8% 196 1.384E-57
3 phalp2_14741
6KOub
 4 43,0% 193 7.371E-55
4 phalp2_22451
TtK5
 1 38,0% 176 2.090E-52
5 phalp2_20891
6SNpy
 16 29,8% 194 2.069E-49
6 phalp2_23352
5BBNt
 14 45,9% 161 7.249E-49
7 phalp2_8458
15NMW
 2 39,8% 163 1.490E-46
8 phalp2_26205
hgNg
 25 42,8% 168 3.413E-45
9 phalp2_8004
5n9mx
 40 27,4% 281 3.413E-45
10 phalp2_31597
4osFp
 9 31,6% 196 3.053E-44

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 5ioYm (239 AA)
Member sequence: A0A2H5BML6 (254 AA)
1 239 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptomyces phage ToriToki
[NCBI] 		2060089 Immanueltrevirus > Immanueltrevirus immanuel3
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MG663585 [NCBI]
CDS location
range 2650 -> 3414
strand +
CDS
ATGGCACGCTTCAGCAAGGCTAAGTGGAAGCCCGTACGCAACTACACGAACAACGGACAGGATCGAGTCCTAGGACTCATCGTCCACATCATGCAGGGAACCCTGGAGGGTTCCCAGTCCTGGTTCGACAACCCCTCCGCTCAGGCGTCCAGCCACTTCGGAACCGGCAAGGACGGAGAGCTGAGGCAGTGGGTCGACACCAGCGATCGAGCCTGGGCACAGGCTGCCGGTAACAGGGACTACCTCTCCATCGAGAACGAGGGCAAGGTCCCGGACAAGCTCACCGCCAAGCAGATCGAGGCCGTGGCCCAGACCTTCGCCTGGGTGGCCAAGACCTACAAGGTTCCCTACCAGGTAGCCGACAAGCCGGGCGAGAAGGGTCTCGGGTTCCATCGCATGGGAGGGGCCGCCTGGGGCGGCCACAGCTGCCCCGGTGACAACATCATCAAGCAGCTCCCCGAGATCGTGGCACGAGCCAAGGAGATCAACAAGGTGACGGCGAAGCCGAAGCCTGTCTACGCTCCGTTCCCCGGCAAGTCCTTCTTCCGCCTCGGCCAGAAGCACAAGCTCATCACTGAGCTTGGCAAGGCTCTGGTGAAGGCTGGCTACAAGGGATACAAGGTGGGTCCAGGCCAGGAGTTCACTCGGGCCGACATCAAGGCCGTGGCCTGGTTCCAGCGCAAGCAGGGCTGGACCGGAGCAGACGCGGACGGGTACCCAGGCCCGGAGACCTGGAAGCGACTGAAGGTCGCACAGCCCAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000ca2ea04_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (5ioYm) rather than this protein.
PDB ID
5ioYm
Method AlphaFoldv2
Resolution 94.30
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50