Protein

Protein accession
A0A1D8ETE4 [UniProt]
Representative
6oM64
Source
UniProt (cluster: phalp2_6225)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MDWTNLNADVTKLMNVHFTPGREGHTIDKIVIHHNGGNLSIDQIWNVWQDREASAHYQVQSDGRIGQLVNDWDTAWHAGDWDANLTSIGIEHADDSTDPWHVSDAAVDAGAHLVAALCRGYNLGRPEWMRNVFPHSHFSATSCPASLAENQRADYMSRAQAYYDGTSVAAAPAAPSAPAGIHVDLPSWTLPAGHFYGLVSGGEDSHGGYYEAERPAIRQIQLWLIRHGYAGAVPDSWADGIYEQPTADAVAAFQRAERPNSTDRWGEVWADDLATMAANNG
Physico‐chemical
properties
protein length:281 AA
molecular weight:30752,2 Da
isoelectric point:4,89
hydropathy:-0,45
Representative Protein Details
Accession
6oM64
Protein name
6oM64
Sequence length
222 AA
Molecular weight
24302,36350 Da
Isoelectric point
5,82790
Sequence
MKNWETLEADEDMILNTHFTPGRSGRNIDKIVIHHNAGNLSIAGCYNVWQTREASAHYQVDSNGRIGQLVWDADTAWHAGDWAANTTSIGIEHADINTNPWQCSDATIDNGAHLVAALCKYYELGRPQWGVNVFPHSRFSATECPASLAGTQNAAYMAKAQYWYDKMSGGNPSTPSTPSQPQQHATNLEALADAVIRGDYGNGDERKQRLGSHDRPAAGKHG
Other Proteins in cluster: phalp2_6225
Total (incl. this protein): 106 Avg length: 257,6 Avg pI: 6,52
Protein ID Length (AA) pI
6oM64 222 5,82790
11u8h 245 5,33539
11vpK 245 5,43821
13DeI 296 6,88192
13cUr 241 5,30481
13pyD 240 5,36403
1HHGO 245 5,24336
1RhvG 293 8,35048
1bEWD 259 7,74866
1bEZX 279 6,55532
1bQSt 278 9,40563
1cugb 272 8,85178
1ekB0 272 9,15182
1eq3E 258 7,74439
1f9Hp 269 5,00333
1j0nw 229 7,77366
1kr8T 245 5,32169
1rkMQ 296 4,80587
21f2q 184 5,02988
2bjGa 245 5,55262
2bkYv 245 5,45463
3ATJO 266 7,21460
3c8Kp 245 5,54757
3cf05 247 5,58701
3dvAF 294 4,51594
3u5GR 287 5,75463
5HDBi 258 9,40821
5LiE9 224 5,36653
5Lz83 215 5,50562
5ou64 263 10,04380
5tDpP 278 8,99013
5tU4n 286 9,87432
6Mrp 301 4,78439
6YPfF 245 5,24336
6Yvbs 245 5,46111
6Z3Gk 245 5,46117
6bF5V 215 6,38753
6bpI7 225 5,43821
6gAdZ 287 5,65675
6kevK 211 5,39689
6ur3J 327 5,57161
6ur6i 304 4,42886
6uzSi 327 5,53648
702UV 245 5,24336
71UHM 259 7,05363
757UB 257 6,36349
75Zil 263 6,86407
7Aela 284 4,84322
7BI1J 272 9,12487
7MxAM 255 4,80587
7RbDW 245 6,54770
7UKbV 245 5,24336
7Vbl2 245 6,69276
7W7HU 245 5,46111
7Yjt1 232 7,80243
7ZzYH 245 5,46111
7cxRm 258 5,44707
7cxSR 241 8,53150
7d1Ln 256 9,33330
7em7u 251 8,30342
7iTg8 250 9,36263
7kBfq 245 6,81405
7lC0Z 163 6,17058
7nJwD 253 8,51526
7oicA 262 6,26885
7qJ5C 281 8,82013
7s0wf 236 9,75660
7sQIX 283 5,02709
7sR6E 284 4,91995
7ubhr 160 6,22770
7ubqY 279 8,91142
7ui7N 280 5,68381
7ujjp 259 5,25814
7umgz 287 9,20720
7w0U2 245 6,53241
7xJ0t 269 9,51503
7yjF4 281 8,60506
84oio 242 5,10570
8cDz2 255 4,94615
8hjFE 243 5,24154
8hjpf 245 5,24336
8nbPi 318 5,38296
8nc1H 323 6,70816
8qUDi 232 5,95754
8qUFl 270 8,37562
A2kw 245 5,48726
CDDL 285 9,20023
HIcw 243 5,04988
HNnI 245 5,24336
KPOc 265 8,63917
KbpD 245 5,46117
O9Bt 245 5,57826
aZlJ 241 9,36953
oChG 255 4,99458
oEkH 211 5,36051
oHf6 245 5,36113
ofXC 237 9,09883
p2Em 243 5,34908
qIgw 227 6,78069
wN4I 245 5,55274
wUMB 245 5,23387
wuaG 266 8,33617
A0A1D8ETX7 284 4,84322
A0A6G6XZQ6 327 6,10277
A0A6G5YMV5 327 6,10277
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_1607
7ZyR0
 345 58,6% 220 3.678E-93
2 phalp2_4921
6qvcZ
 45 64,0% 181 1.177E-91
3 phalp2_30585
5Zqsx
 178 60,9% 187 8.360E-82
4 phalp2_2841
8IAfz
 3 29,2% 205 2.129E-38
5 phalp2_20487
3X7ua
 81 34,6% 208 1.018E-37
6 phalp2_7058
8iEHQ
 9 33,6% 196 5.721E-33
7 phalp2_1605
81mMl
 270 33,3% 168 1.301E-26
8 phalp2_18388
5HE4u
 2 29,4% 214 3.304E-26
9 phalp2_22065
5F1i1
 7 32,2% 161 5.396E-25
10 phalp2_1359
1dk8I
 5 28,4% 197 1.938E-22

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 6oM64 (222 AA)
Member sequence: A0A1D8ETE4 (281 AA)
1 222 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510|PF08230

Taxonomy

  Name Taxonomy ID Lineage
Phage Propionibacterium phage B3
[NCBI] 		1897533 Anatolevirus > Anatolevirus B3
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KX620749 [NCBI]
CDS location
range 19121 -> 19966
strand +
CDS
ATGGACTGGACCAATCTGAACGCTGACGTGACGAAGCTGATGAACGTGCACTTCACCCCCGGGCGTGAAGGCCACACGATCGACAAGATCGTGATTCACCACAACGGCGGCAACCTGAGCATCGACCAGATCTGGAATGTGTGGCAGGACCGTGAAGCATCGGCCCACTATCAGGTGCAATCTGATGGCCGTATCGGCCAGCTCGTCAACGACTGGGACACCGCCTGGCACGCCGGCGACTGGGACGCCAACCTGACCTCGATCGGCATCGAGCACGCCGACGACTCGACCGACCCGTGGCATGTGTCTGATGCTGCCGTCGATGCCGGCGCGCACCTGGTGGCTGCACTGTGTCGCGGCTACAACCTTGGCCGGCCGGAGTGGATGCGCAACGTCTTCCCGCACTCGCATTTCTCGGCGACCTCGTGCCCGGCCTCGCTGGCCGAGAATCAGCGCGCGGATTACATGTCACGGGCTCAGGCCTACTACGACGGCACTTCAGTGGCTGCCGCACCTGCTGCCCCGTCGGCACCTGCCGGGATCCACGTCGATCTTCCGAGCTGGACCCTCCCCGCAGGCCACTTCTACGGTCTCGTGAGCGGTGGCGAGGATTCGCACGGCGGCTACTACGAGGCCGAGCGACCGGCGATCCGCCAGATCCAACTGTGGCTCATCCGCCACGGCTACGCCGGCGCGGTGCCTGACAGTTGGGCGGACGGCATCTACGAGCAGCCCACCGCCGACGCCGTAGCGGCCTTCCAGCGCGCCGAGCGACCAAACTCCACGGACCGGTGGGGCGAGGTCTGGGCCGACGATCTGGCCACCATGGCCGCCAACAACGGCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6oM64) rather than this protein.
PDB ID
6oM64
Method AlphaFoldv2
Resolution 88.15
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50