Protein

Protein accession
A0ZS57 [UniProt]
Representative
7yFAa
Source
UniProt (cluster: phalp2_20990)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGERLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDKAKSVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNNKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKKDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYTPDKNNVPYKKETGYYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSAV
Physico‐chemical
properties
protein length:484 AA
molecular weight:53773,9 Da
isoelectric point:9,28
hydropathy:-0,48
Representative Protein Details
Accession
7yFAa
Protein name
7yFAa
Sequence length
308 AA
Molecular weight
34727,87560 Da
Isoelectric point
8,90503
Sequence
MLMTRNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPDSISVKDKAKGIIKQATAKKEAVIKPKRIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKKVYKMLLKITWDK
Other Proteins in cluster: phalp2_20990
Total (incl. this protein): 82 Avg length: 428,8 Avg pI: 9,14
Protein ID Length (AA) pI
7yFAa 308 8,90503
73a4P 176 8,80704
75Ban 334 7,74155
78B87 220 9,34200
79Nar 183 9,28475
7Rfws 257 9,36179
7Ribo 188 9,28456
7a6Vp 334 6,45187
7aH2t 183 9,28475
7aN38 327 9,03365
7bAZA 435 9,16400
7c3Bd 189 9,46107
7duLE 216 9,18644
7f4NS 299 7,09171
7kRuA 265 9,34890
7kroY 304 8,60790
7l17s 304 8,91438
8tcB1 255 9,53844
W5RAM3 484 9,29313
A7TWE3 484 9,28617
A0A068A229 484 9,18915
A0A6F8Z5Y7 141 7,64083
A0A411BM53 484 9,29313
A0A2K9VBR6 484 9,32730
A0A345AQC9 484 9,28617
A0A514U6E6 484 9,15839
B7T0L1 484 9,37314
A0A513ZXD2 484 9,29313
A0A6F8Z5Y5 341 9,56229
Q8SDN4 484 9,24246
R4WAL6 484 9,27934
B5WZR3 484 9,28617
O80064 484 9,28617
U5U7D2 484 9,27263
Q4ZCR7 484 9,18915
E9LT66 484 9,27934
A0A0N6WMR3 484 9,29313
A0A1D8H0F0 484 9,15839
A0A1X9IGW0 484 9,15839
A0A2D1G5T1 484 9,28617
A0A2I6PDH0 484 9,28617
A0A2I6PDK1 484 9,28617
A0A2I6PDT5 484 9,28617
A0A2I6PE01 484 9,28617
A0A2I6PE84 484 9,15839
A0A2I6PED1 484 9,28617
A0A2I6PET7 484 9,15839
A0A2I6PEZ2 484 9,15839
A0A2I6PF47 484 9,28617
A0A513ZXM7 484 9,29313
A0A5P8D297 484 8,97479
C5I684 484 9,28617
G4KNR5 484 9,28617
G4KNV7 484 9,28617
I1W5Y1 484 9,29313
M1TB12 484 9,27934
Q9B0C3 484 9,15839
A0A976SVM9 484 9,27940
A0A976XQQ4 484 9,20223
A0A977R895 484 9,18915
A0A977TEW3 381 8,95397
A0A977TFI8 484 9,14524
A0A9E7SMQ0 484 9,34142
A0A9E8GIR1 484 9,33433
A0A9X9JYN3 484 9,19566
A0A9X9JZZ0 484 9,27934
A0A9X9P2J5 484 9,12739
A0AA48LI37 484 9,33433
A0AA96J2J9 484 9,29313
A0AAE7ANI6 484 9,26612
A0AAE8ZA40 484 9,27940
A0AAE9T6T0 484 9,20223
A0AAX3C8N4 484 9,27934
A0EWN5 484 9,32730
A0EX84 484 9,32730
A4ZFD7 484 9,32730
A7TWL1 484 9,35908
Q4ZBP3 484 9,32730
Q4ZCK1 484 9,24246
Q4ZDK0 484 9,32730
Q6R861 484 9,32730
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_22245
7e47k
 317 62,1% 301 7.188E-141
2 phalp2_10799
3WA57
 47 29,3% 218 5.253E-12
3 phalp2_35363
7xThI
 2 27,7% 288 4.009E-11

Domains

Domains [InterPro]
CHAP
Ami3
Representative sequence (used for alignment): 7yFAa (308 AA)
Member sequence: A0ZS57 (484 AA)
1 308 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520, PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage phiPVL108
[NCBI] 		360398 Peeveelvirus > Peeveelvirus PVL108
Host Staphylococcus aureus
[NCBI] 		1280 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AB243556 [NCBI]
CDS location
range 38494 -> 39948
strand +
CDS
ATGTTGATAACAAAAAACCAAGCAGAAAAATGGTTTGATAATTCATTAGGGAAGCAGTTCAATCCTGATTTGTTTTATGGATTTCAGTGTTACGATTACGCAAATATGTTTTTTATGATAGCAACAGGCGAAAGGTTACAAGGTTTATACGCTTATAATATTCCATTTGATAATAAAGCAAGGATTGAAAAATACGGGCAAATAATTAAAAACTATGATAGCTTTTTACCGCAAAAGTTGGACATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACATGTTGAAATTGTTGAGAGCGCTAATCTAAACACTTTCACATCGTTTGGCCAAAATTGGAATGGTAAAGGTTGGACAAATGGCGTTGCGCAACCTGGTTGGGGTCCCGAAACCGTTACAAGACATGTTCATTATTACGATGACCCAATGTATTTTATTAGATTAAATTTCCCAGATAAAGTAAGTGTTGGAGATAAAGCTAAAAGCGTTATTAAGCAAGCAACTGCCAAAAAGCAAGCAGTAATTAAACCTAAAAAAATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTCGGAAACGGAACAAATGAACGTGATTTTATCCGTAAATATATAACACCAAATATCGCTAAGTATTTAAGACATGCAGGTCACGAAGTTGCATTATATGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACTGCTTACGGTGTTAATGTAGGAAATAATAAAGATTATGGCTTATATTGGGTTAAATCACAGGGGTATGACATTGTTCTAGAGATTCATTTAGACGCAGCAGGAGAAAGTGCAAGTGGTGGGCATGTTATTATTTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTTATTAAAAATAACTTAGGACAAATAAGAGGTGTAACACCTCGTAATGATTTACTGAACGTTAATGTATCAGCAGAAATAAATATCAATTATCGTTTATCTGAATTAGGTTTTATTACTAATAAAAAAGATATGGATTGGATTAAGAAGAATTATGACTTGTATTCTAAATTAATAGCTGGTGCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAAAACCAAAAGAATCCGCCAGTGCCAGCAGGTTATACACCCGATAAAAATAATGTACCGTATAAAAAAGAAACTGGTTATTACACAGTTGCCAATGTTAAAGGTAATAACGTGAGGGATGGCTATTCAACTAATTCAAGAATTACAGGTGTATTACCTAATAACGCAACAATCAAATATGACGGAGCATATTGCATCAATGGGTATAGATGGATTACTTATATTGCTAATAGTGGACAACGTCGTTATATTGCTACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGCAGTTTTGGTAAGTTTAGTGCAGTTTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
A0ZS57
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
M1TB12
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi00000d9e10_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7yFAa) rather than this protein.
PDB ID
7yFAa
Method AlphaFoldv2
Resolution 89.01
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50