Protein

Protein accession
A0A4D6BE62 [UniProt]
Representative
7vSPi
Source
UniProt (cluster: phalp2_29169)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTTVNEALNNVRAQVGSGISVGNGECYALASWYERMISPDATVGLGAGVGWVSGAIGDTISAKNIGSSYNWQANGWTVSTSGPFKAGQIVTLGATPGNPYGHVVIVEAVDGDRLTILEQNYGGKRYPVRNYYSATSYRQQVVHHITPPGTITQSAPNLAGSRSYRETGTMTVTVDALNVRRAPNTSGEIVAVYKRGESFDYDTVIIDVNGYVWVSYIGGSGKRNYVATGATKDGKRFGNAWGTFK
Physico‐chemical
properties
protein length:245 AA
molecular weight:26122,0 Da
isoelectric point:9,08
hydropathy:-0,26
Representative Protein Details
Accession
7vSPi
Protein name
7vSPi
Sequence length
242 AA
Molecular weight
25514,04290 Da
Isoelectric point
8,85281
Sequence
MTTVNEALNNVRALVGSGQSIGNGECYALASYYERMISPDATVGLGAGIGWVSGAVGDTIAAHNIGTSYNWSANGWSVSTSGPFKAGQIVTLRGGTYGHVVIIEAVNGDQLVVIEQNYGGKRYPVRNYYSASAYARDVAHYITPPGAVSNPTPAASGAKKYTEHGTMTVTVDAINVRRAPNTSGQVVAQYTKGQSFEYDTVIIDVNGFVWVSYIGGSGKRNYVATGATKNGERFGSAWGTFK
Other Proteins in cluster: phalp2_29169
Total (incl. this protein): 45 Avg length: 245,7 Avg pI: 6,64
Protein ID Length (AA) pI
7vSPi 242 8,85281
1czfy 248 4,13705
1km2c 248 4,31694
5Qf5J 248 4,16575
5TcUS 248 4,24328
5WGuJ 248 4,18826
60vUm 248 4,08766
6iFRc 248 4,18826
6mf4v 248 4,10175
6r8ws 248 4,24328
6tUhm 248 4,20310
6vw9z 248 4,12227
7166d 248 4,18826
71VRu 245 9,07652
725rD 249 6,73448
72jqV 245 9,40421
75Ceh 245 9,53998
75WdW 249 4,16575
75uuD 245 6,72169
75vPy 245 9,29797
75vRG 245 7,76679
75vXj 245 9,07684
75vb0 245 9,04080
75wab 245 9,07665
75xJP 245 8,86842
79y9S 245 8,85165
7gECr 245 8,54072
7h2Qr 245 7,69938
7lVff 245 9,07684
7rjsZ 248 4,19946
7rr94 248 4,08766
7vSP8 220 6,72368
7z5NL 245 7,76639
7z5hH 243 9,09676
8nRAr 248 4,18826
KLoE 248 4,17485
Oghw 248 4,24328
ytud 248 4,18826
A0A4D6ANN1 242 8,85281
A0A1X9I6R1 245 9,07652
A0A4D6ARS3 249 6,73448
A0A4D6BB83 245 6,72169
M1PKZ3 245 8,54072
M1NS67 245 9,05815
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5002
1dLD0
 578 32,2% 254 7.663E-30
2 phalp2_36492
1kc1M
 27 26,3% 190 1.215E-06

Domains

Domains [InterPro]
CHAP
SH3_5
Representative sequence (used for alignment): 7vSPi (242 AA)
Member sequence: A0A4D6BE62 (245 AA)
1 242 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF05257, PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptococcus phage Javan580
[NCBI] 		2548272 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK448989 [NCBI]
CDS location
range 17341 -> 18078
strand +
CDS
ATGACAACAGTAAATGAAGCCTTAAATAACGTCCGTGCCCAAGTCGGGTCTGGCATATCAGTAGGTAACGGCGAATGCTACGCTTTGGCTAGTTGGTACGAGCGCATGATTAGTCCGGATGCAACTGTCGGACTTGGCGCTGGTGTGGGCTGGGTCAGCGGTGCAATCGGCGATACAATCTCTGCCAAAAACATCGGCTCATCATACAACTGGCAAGCTAACGGCTGGACAGTTTCCACATCTGGTCCATTTAAAGCAGGTCAGATTGTGACGCTTGGGGCAACACCAGGAAACCCTTACGGACATGTGGTAATCGTCGAAGCAGTGGACGGCGATAGATTGACTATTTTGGAGCAAAACTACGGCGGGAAACGTTATCCCGTCCGTAATTATTACAGCGCTACAAGCTATCGTCAACAAGTCGTGCACCACATCACACCGCCTGGAACGATTACACAGTCAGCACCCAACCTTGCAGGCTCTCGTTCCTATCGCGAGACGGGCACTATGACTGTCACGGTCGATGCTCTCAATGTTCGCAGGGCGCCAAATACTTCAGGCGAGATTGTAGCAGTATACAAGCGTGGTGAGTCATTTGACTATGATACTGTCATCATCGATGTCAATGGCTATGTCTGGGTGTCTTACATAGGCGGCAGCGGCAAACGTAACTACGTTGCGACGGGCGCTACCAAAGACGGTAAGCGTTTCGGCAATGCTTGGGGTACATTTAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0003824 catalytic activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
A0A1X9I5W6
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
A0A4D6BE62
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7vSPi) rather than this protein.
PDB ID
7vSPi
Method AlphaFoldv2
Resolution 87.12
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50