Protein

Protein accession
A0A0A8WI59 [UniProt]
Representative
6ql8e
Source
UniProt (cluster: phalp2_259)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVDIIKMLTKKKCYPNKNSPKFIVIHETDNEDKGADAKRHAQALNNGNLEASVHYYVDDKVIYQTLDHKDGAWAVGKSYGTALVAGVTNYNSINIEICVNRDGNYTKARQNAIDLTRKLMKDLNISTDKVIRHYDAKRKYCPRKMLDNPKLWVDFKDKIKNDGGVNSMIKYTIVYDGEVDRIPAEVIGWNYKKEECRVCDIKEYIPGQTQNLYIVGGGACNKIGSITKEHYTMIKGNDRFETLYKALDFIKK
Physico‐chemical
properties
protein length:252 AA
molecular weight:28844,8 Da
isoelectric point:9,05
hydropathy:-0,63
Representative Protein Details
Accession
6ql8e
Protein name
6ql8e
Sequence length
252 AA
Molecular weight
28468,29160 Da
Isoelectric point
9,00444
Sequence
MVDIIKMLTKKKCYPNKNNAKFIVIHETDNEDKGADAKRHAQALNNGNLEASVHYYVDDKVIYQTLDHKDGAWAVGKSYGTALVAGVTNYNSINIEICVNRDGNYTKARQNAIDLTRKLMKDLNISADKVIRHYDAKKKYCPRKMLDNPKLWVDFKDKIKNDGGVNSMIKYTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYVPGQTQNLYVVGGGACEKIGSITKEHYTVIKGNDRFDTLYKALDFIKK
Other Proteins in cluster: phalp2_259
Total (incl. this protein): 14 Avg length: 260,4 Avg pI: 9,01
Protein ID Length (AA) pI
6ql8e 252 9,00444
74nW8 252 8,93237
74sj5 252 9,08980
74uhp 252 9,04912
74vCr 252 9,03462
7dReF 252 9,03474
7eax0 248 9,20159
7xlKt 252 8,97118
A0A6B9J229 268 8,89266
A0A0A8WIC2 268 8,44834
A0A0A8WE15 269 8,99239
E2ELK8 268 9,14750
Q331X1 309 9,28869
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_24299
3nIle
 36 39,6% 174 3.294E-33
2 phalp2_7826
aMK
 29 42,5% 160 6.129E-33
3 phalp2_33479
7swgL
 97 42,2% 168 1.001E-31
4 phalp2_20292
2aQ1n
 8 41,0% 185 1.001E-31
5 phalp2_29669
8sfID
 1005 39,7% 176 4.129E-30
6 phalp2_29544
3KzTO
 225 40,2% 179 3.606E-29
7 phalp2_29527
23SbO
 38 41,4% 169 3.606E-29
8 phalp2_3987
7ylun
 65 35,5% 256 6.898E-27
9 phalp2_36615
23Gpv
 52 34,0% 191 1.300E-24
10 phalp2_37301
7Ya12
 3 29,9% 244 1.769E-24

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 6ql8e (252 AA)
Member sequence: A0A0A8WI59 (252 AA)
1 252 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Clostridium phage phiCD481-1
[NCBI] 		1582153 Sherbrookevirus > Sherbrookevirus CD4811
Host Peptoclostridium difficile
[NCBI] 		1496 Firmicutes > Clostridia > Clostridiales > Peptostreptococcaceae > Peptoclostridium >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LN681538 [NCBI]
CDS location
range 19007 -> 19765
strand +
CDS
ATGGTAGATATAATAAAGATGTTAACAAAGAAAAAGTGTTATCCAAACAAGAATAGTCCTAAATTCATAGTGATTCATGAGACTGATAATGAGGACAAGGGAGCAGATGCTAAGAGACATGCACAAGCACTTAATAATGGTAATTTAGAAGCAAGTGTTCATTACTATGTTGATGATAAAGTAATATATCAAACATTAGACCATAAAGATGGAGCGTGGGCAGTTGGTAAAAGCTATGGAACTGCATTAGTTGCAGGAGTTACAAATTACAATAGTATTAATATAGAAATATGTGTAAATCGTGATGGTAATTACACAAAAGCAAGACAAAATGCAATAGATTTAACTAGAAAACTCATGAAAGATTTAAATATTAGTACAGATAAAGTAATAAGACACTATGATGCTAAGAGAAAATATTGTCCTCGTAAGATGCTTGATAATCCAAAATTATGGGTAGATTTTAAAGATAAGATAAAAAATGATGGGGGTGTGAATAGTATGATAAAATACACAATAGTCTATGATGGAGAGGTTGACAGAATACCAGCCGAGGTTATTGGTTGGAATTATAAGAAAGAAGAATGTAGAGTATGTGATATAAAAGAATATATACCTGGTCAGACGCAAAATCTGTATATTGTAGGTGGGGGAGCATGTAATAAGATAGGTTCTATAACAAAAGAACATTATACAATGATAAAGGGTAATGATAGGTTTGAAACACTTTACAAAGCATTAGATTTTATTAAGAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
A0A0A8WI59
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi00038cdd84_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (6ql8e) rather than this protein.
PDB ID
6ql8e
Method AlphaFoldv2
Resolution 94.53
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50