Protein

Protein accession
A0A0U3U037 [UniProt]
Representative
83ZcC
Source
UniProt (cluster: phalp2_20199)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MEIKQMLVPVSRYSVLCPYEMNPTEITFHNTYNDAPAINERNNVANNSTGTSFHIAVDDKEAIQLIPFNRNAWHAGDGTNGRGNRHSIGVEICYSQSGGARYRKAELNAVEVIAQLMIQFDIPISKVKTHQERNGKYCPHRMLDEGRVQWFKNQCANRASSIKNSNKTQETGKVEIIVNKFNKVVTYEFGTALVPEMLGMMDALGYESRIISYGDKQGLVRFETAYRQGNELDKATAWLDAKGLKYFYTKE
Physico‐chemical
properties
protein length:251 AA
molecular weight:28472,9 Da
isoelectric point:8,55
hydropathy:-0,55
Representative Protein Details
Accession
83ZcC
Protein name
83ZcC
Sequence length
336 AA
Molecular weight
36266,76610 Da
Isoelectric point
9,57228
Sequence
MAYKYVEKLIPSSKYSLKAPYAMTPQYVTIHNTSNDAPAVNEVSYASGNTAPTGFHYAIDDKEAILMIPLNRTAFHAGDGASGAGNRKSIGIEICYSKSGGARYEAAEENAVYLAARLLYKYGLDISRLKKHQDWSGKYCPHRILDKKSWNDFKSRVNWVLEEIKAGRVNGSLTNGSTQPSGSAQSNTVNKPSAPVINESTSLTGSNFSVKIITDSLNIRKTASFSAPVVGTVGKGGVYAIVETSNGMGRLKSGTGWISMSTKYVEKVAAKPATTEYKVKVVNCDSLNVRSGAGASHKVVQTVKPGDVFTIVEEKDGWLKLKSGVGWISKAYTKKL
Other Proteins in cluster: phalp2_20199
Total (incl. this protein): 8 Avg length: 315,5 Avg pI: 8,94
Protein ID Length (AA) pI
83ZcC 336 9,57228
1c43B 338 9,31608
3PSDV 346 9,08058
3gLvL 355 7,64220
7dzeE 327 8,89375
7xMbf 327 8,89375
jr0c 244 9,59510
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_19215
3ui7r
 7 33,9% 389 4.715E-74
2 phalp2_14662
5ObrM
 161 31,8% 327 3.703E-38
3 phalp2_11198
68WMu
 78 33,7% 255 6.845E-38
4 phalp2_22264
7pxLN
 64 28,7% 313 1.259E-35
5 phalp2_19907
6axIZ
 11 29,2% 339 7.480E-31
6 phalp2_11225
6wIAx
 104 34,1% 275 1.374E-30
7 phalp2_3987
7ylun
 65 28,6% 265 2.116E-29
8 phalp2_27054
2dgKz
 1 31,2% 224 1.306E-28
9 phalp2_4901
5Zr0m
 117 30,2% 278 5.936E-28
10 phalp2_2331
4MNkD
 32 29,3% 259 1.649E-26

Domains

Domains [InterPro]
Ami2
Unannotated
SH3_3
Representative sequence (used for alignment): 83ZcC (336 AA)
Member sequence: A0A0U3U037 (251 AA)
1 336 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage vB_BceS-MY192
[NCBI] 		1759525 Hubeivirus > Hubeivirus MY192
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KT725776 [NCBI]
CDS location
range 39583 -> 40338
strand +
CDS
ATGGAAATTAAACAAATGTTAGTACCTGTAAGTCGTTACTCCGTCTTATGCCCGTATGAAATGAATCCAACAGAAATCACATTTCATAACACGTACAATGACGCACCGGCTATAAATGAGCGTAATAATGTCGCTAACAATAGCACCGGAACATCATTTCATATCGCTGTGGATGATAAGGAAGCTATTCAGTTAATTCCTTTTAACAGAAACGCATGGCACGCTGGAGATGGTACTAATGGAAGAGGGAATCGTCATAGTATTGGGGTTGAAATTTGTTATTCACAATCAGGCGGAGCAAGATATCGCAAAGCTGAATTGAATGCAGTTGAAGTGATTGCTCAATTAATGATTCAATTTGATATACCGATTAGTAAAGTTAAGACTCATCAAGAGAGAAATGGGAAGTACTGTCCACATCGAATGTTAGATGAAGGACGTGTTCAGTGGTTTAAAAATCAATGTGCAAATAGAGCATCTAGTATTAAAAATTCAAATAAAACACAAGAGACAGGAAAGGTGGAGATTATAGTGAATAAATTTAATAAAGTTGTTACGTATGAATTTGGGACAGCGTTAGTACCTGAGATGTTAGGAATGATGGATGCCCTAGGTTATGAATCTCGTATTATCTCATATGGAGATAAACAAGGATTAGTTAGATTTGAAACAGCATATCGCCAAGGTAACGAGCTGGACAAGGCTACAGCGTGGTTAGATGCTAAAGGACTTAAATACTTCTATACAAAAGAATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
A0A0U3U037
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
A0A1B1P7T9
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
A0A1B1P8F4
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi000012874b_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (83ZcC) rather than this protein.
PDB ID
83ZcC
Method AlphaFoldv2
Resolution 85.46
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50