Protein

Protein accession
A0A4D6B228 [UniProt]
Representative
NzwP
Source
UniProt (cluster: phalp2_7974)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MATLNDILNYAESLANQGVGADADGAYGTQCVDLPNSISINFFGKALWGNAIDLLNSAAGLGYEVVYDAVGVNPRAGAIFVMDTTYLYGHPYGHTGIVIEDSDGVTMKTIEQNIDGNADSLYVGGPARYNTRNFDGIVGWFYFPTDDTSVAFEQPEPSEPLTIESNGFHPETGTFTVEVSALNVRVEAGLGAEIVAVYSAGQEINYDGWIDNDGYIWISYIGGSGNRRYVAVGKSENGQRITDFGSFK
Physico‐chemical
properties
protein length:248 AA
molecular weight:26621,9 Da
isoelectric point:4,12
hydropathy:-0,13
Representative Protein Details
Accession
NzwP
Protein name
NzwP
Sequence length
161 AA
Molecular weight
18325,94120 Da
Isoelectric point
4,39351
Sequence
AVFVMDTTYIYGHPFGHTGVVIEDSDGYTMKTIEQNVDGNADALYVGGPARYMERNFDGIVGWFYYPTDDNEVVEENSDLISLPEVRTYTVGVDKLNIRNAPSTEAEIVGSYEKGEEFNYMEFCNANGYEWLSYISNSGVRRYVASMDLETFETHGTWRKK
Other Proteins in cluster: phalp2_7974
Total (incl. this protein): 4 Avg length: 204,3 Avg pI: 4,34
Protein ID Length (AA) pI
NzwP 161 4,39351
7rCOZ 167 4,20918
A0A8S5MGE2 241 4,63246
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5002
1dLD0
 578 62,4% 149 4.536E-68
2 phalp2_23379
5JYK7
 2 35,0% 120 6.278E-17
3 phalp2_18246
7DJw9
 1 27,3% 150 1.343E-04

Domains

Domains [InterPro]
Unannotated
SH3_5
Representative sequence (used for alignment): NzwP (161 AA)
Member sequence: A0A4D6B228 (248 AA)
1 161 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptococcus phage Javan372
[NCBI] 		2548133 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK448920 [NCBI]
CDS location
range 31403 -> 32149
strand +
CDS
ATGGCAACACTAAATGACATTCTAAATTATGCAGAATCTTTGGCAAATCAAGGTGTGGGAGCTGATGCAGATGGTGCATACGGAACCCAATGTGTGGACCTACCAAATTCAATTTCTATCAACTTCTTTGGGAAAGCTCTCTGGGGCAATGCTATTGACCTACTTAATTCAGCCGCTGGGTTAGGGTATGAAGTAGTATATGATGCAGTAGGAGTCAACCCACGAGCAGGAGCCATCTTTGTCATGGATACAACTTACCTGTACGGCCATCCTTATGGTCACACAGGAATTGTAATTGAGGACAGCGATGGAGTCACCATGAAAACCATTGAACAGAACATTGATGGGAATGCTGATTCCCTCTATGTTGGAGGTCCTGCACGATACAACACACGCAACTTTGACGGAATTGTTGGATGGTTCTATTTCCCAACTGATGACACATCTGTGGCATTCGAACAGCCAGAACCATCAGAACCATTGACAATTGAATCAAATGGATTCCATCCAGAAACAGGGACATTCACTGTTGAGGTGTCTGCTCTAAATGTACGAGTTGAAGCAGGTCTTGGAGCTGAAATTGTAGCTGTATACAGTGCAGGTCAAGAAATCAACTATGATGGATGGATTGACAATGATGGCTACATTTGGATCTCTTACATTGGCGGTTCTGGAAATCGCAGATATGTGGCAGTGGGGAAATCCGAAAACGGGCAACGTATCACAGACTTTGGATCTTTTAAATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0016787 hydrolase activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi00066b2e61_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (NzwP) rather than this protein.
PDB ID
NzwP
Method AlphaFoldv2
Resolution 90.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50