Protein

Protein accession
5mlgT [EnVhog]
Representative
4SR8r
Source
EnVhog (cluster: phalp2_28777)
Protein name
5mlgT
Lysin probability
98%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MVTSQQALKKYGQPSPSNPNLVLWDVPAELEIGVIPKRIYCNKDMVAPLTAAFKALISTGCVTELKTWDGCFNIRKKRGLASMSLHSWAIAIDVNAFENGLNQTPKLSKLFVSCFTNNGFDWGGTWTRKDGMHFQLSKI
Physico‐chemical
properties
protein length:139 AA
molecular weight:15465,8 Da
isoelectric point:9,30
hydropathy:-0,13
Representative Protein Details
Accession
4SR8r
Protein name
4SR8r
Sequence length
162 AA
Molecular weight
17936,16220 Da
Isoelectric point
6,56589
Sequence
VYAVIAPTSYAGVLHVYGDPARFLREDGTLSHLWEAQMTKVSFPRPLPLGWAKETLAKAARVNIVIAEETERVFALLEREGLWALLKTYDGGYTWRTQRGSTTKLSMHSFGAALDFNAETNALGTPGDMSAGIVQVFESCGWTWGGRFHRPDPMHFQFASGV
Other Proteins in cluster: phalp2_28777
Total (incl. this protein): 700 Avg length: 145,1 Avg pI: 8,67
Protein ID Length (AA) pI
4SR8r 162 6,56589
11Dz0 151 9,35722
11Ktx 143 7,66152
12Aoy 139 8,92934
12D83 139 9,19553
12HYP 139 9,07994
12J6U 142 9,01141
14Mrd 171 7,63395
15hCV 139 6,88868
189cC 149 8,27924
18Mzo 138 9,30113
18hvd 138 9,19340
18mxE 138 8,78538
193us 139 9,30074
19Nlr 142 9,11662
19Qjz 138 7,64720
1AKPf 161 7,61974
1AOHS 124 8,76256
1CukZ 159 9,74415
1DUOL 159 6,40623
1I14R 139 8,91335
1IL1F 142 8,70602
1IQAa 154 9,48029
1IQuF 138 8,27125
1IR7q 142 8,92940
1ISQ7 138 9,32053
1J6qn 142 7,68255
1JQT8 139 9,34941
1Jc4s 121 9,33639
1JvG1 139 9,27856
1JxVf 142 8,67959
1KAZ1 139 8,26551
1KC72 138 9,03049
1KTnw 157 8,91651
1KUSa 138 6,71697
1KYma 146 7,68335
1KjON 142 9,15859
1Kpom 142 8,37646
1LChc 142 8,92902
1LF5F 142 9,25329
1LXmq 141 9,07949
1LYkP 139 9,34909
1La54 140 6,71328
1LgpM 139 9,34941
1Ljx1 141 9,07949
1LkpF 151 9,34916
1LlNC 139 9,30074
1LmfG 142 8,71956
1M0WQ 140 6,71708
1MKBJ 139 9,30106
1MLVL 148 8,94520
1MLdr 138 8,28756
1MlA2 154 8,69268
1Mo2t 147 8,70531
1MoZe 150 8,65599
1Ms3Y 151 9,21874
1MtrC 139 6,55151
1QGa6 138 9,07955
1QR38 170 8,32192
1T0qy 143 6,81132
1U3Gb 143 8,27531
1WRr4 140 9,06259
1WTxe 139 8,44099
1XbCo 142 8,35583
1Xe8O 141 9,11643
1XgyW 144 9,07949
1ayNJ 144 9,20243
1b2i5 161 8,88350
1cWgY 143 7,66902
1cXHG 160 9,16897
1cXNJ 141 9,17522
1d0Py 154 9,24330
1eyB8 139 9,12564
1hmKx 172 5,14014
1howH 138 9,09754
1iwRq 139 9,16407
1jYRs 160 8,86042
1lbum 158 5,87763
1loGO 140 5,88189
1lwOP 138 8,35602
1lwPk 140 9,02443
1nBKI 142 7,72092
1nrry 147 8,79982
1p3AW 147 9,19269
1pC10 136 9,50891
1pVjs 163 8,76043
1pyQ9 136 9,26548
1q77N 141 8,92921
1q7SM 138 8,94507
1q7kv 139 9,21848
1rISu 142 8,92895
1rxPj 158 9,42819
1tMEc 138 9,34941
1uPJV 142 9,45895
1vFif 138 9,12345
1wTjz 139 8,95609
1wn0R 138 9,25342
1wr7a 138 9,25342
1xMWS 139 9,06704
1zn06 139 9,25561
20sJb 140 9,15910
20xCT 154 9,51735
20yje 139 8,60816
24RLI 139 6,94552
24Std 138 8,56896
24yIN 142 9,25329
25MSd 141 8,74870
25NND 140 9,15898
27TLA 138 9,09767
27uOt 149 9,25342
29KMP 139 9,30087
2B2bS 161 5,21193
2CMTE 141 6,27806
2CMuf 178 5,00742
2F6zX 142 9,38964
2GQdY 140 8,94546
2Gnpd 138 7,70802
2HjbI 179 8,20426
2LHPf 141 8,35950
2NybW 140 9,13725
2PVYJ 143 7,04550
2Qopi 152 9,43819
2SuJ 139 8,70531
2WFZ8 139 9,23208
2X6IY 139 9,04126
2XUDW 155 8,38896
2YH8R 202 6,58465
2Z3QX 142 9,11688
2ZFty 140 9,38906
2ZkTL 155 9,56429
2ZqaV 138 8,82754
2an4T 139 8,37356
2aqwF 138 9,19553
2cH0n 143 9,47049
2cO1u 161 5,91935
2cOOx 139 9,34941
2cUFH 149 8,94900
2gw79 156 9,30048
2hSh9 138 9,17393
2ha82 208 9,53605
2jn2K 138 9,21919
2krvY 154 9,86071
2ln0y 144 7,65498
2pNFT 153 9,19179
2ptnC 139 9,23144
2qPK 160 9,41556
2sS9n 136 8,33114
2sYLs 153 8,72697
2sfOD 143 7,04073
2shvV 139 8,80865
2sreO 139 8,72227
2tD9z 162 6,95564
2tSmc 156 9,37868
30B94 138 9,15330
310Q5 142 9,40034
313K3 140 9,09741
324M7 149 8,94249
32RvQ 140 9,41498
32zZV 152 9,51613
34ogL 142 9,20223
35ZwB 178 4,97650
36BIv 140 7,74991
37YJy 149 9,51742
37YQT 154 9,54263
37e1h 139 8,80730
38dNl 139 8,92985
38gEY 140 9,03881
38ryM 138 9,34303
3Bo7N 143 7,60519
3EvV 159 9,06511
3Fjs3 143 8,74741
3NSFD 157 7,04465
3Qe9c 152 6,56908
3S8pZ 157 5,66187
3SaPl 178 9,31705
3T1mE 137 9,54624
3T86v 149 8,84372
3TWQp 138 9,01083
3U1F0 143 8,66837
3UZ7a 139 8,69287
3Uuz1 138 9,37552
3VYiV 138 9,19334
3W07g 142 9,42839
3Ws4W 136 8,99271
3WuFN 149 9,09709
3XRHP 136 8,99290
3Y3HN 154 9,57873
3Y4GF 147 9,30164
3Y4WU 147 7,65430
3Y9i0 143 5,49516
3Yz5L 172 5,79408
3Zpgr 147 9,29687
3aDRg 171 6,19667
3bD3i 143 9,36547
3dBDA 142 6,04127
3durI 141 5,37927
3g675 165 7,01293
3hzfb 147 9,35296
3iMgq 157 9,33736
3iOqD 182 9,14228
3mwDg 152 8,94133
3n3g 154 9,67891
3niIk 150 8,96557
3nofu 142 9,02449
40TQL 138 9,34252
41YOr 138 9,40544
41sWd 143 8,27544
43Opa 139 9,27676
43Uci 138 9,62224
43W6 146 7,69091
43zxA 138 9,45869
445z0 139 8,79170
447du 144 9,17361
44H1w 157 9,06446
44Jcj 138 9,27012
44KlD 155 9,11649
44fWW 139 8,36995
4540w 169 5,93248
45Qto 138 9,27579
463ew 139 8,80678
46HQv 139 6,27465
46IOe 139 9,27856
46fXa 142 8,45356
46iHV 139 8,88363
46ofd 139 8,94255
470lx 138 8,86287
471Kl 142 8,71853
472M8 139 7,67243
47Nn4 138 8,37420
47cUa 138 8,45015
47eyI 139 9,19579
488I5 138 8,98001
48BW5 139 9,27824
48MwB 152 9,35103
48XV3 119 6,70600
48YVS 142 6,82008
48fSR 141 9,23834
48hJS 140 8,74128
496MI 142 9,38964
497Kh 171 8,83057
497X3 138 8,99439
498rN 138 9,45508
49HWW 141 9,11533
49UL8 142 9,18270
49VI2 140 8,86764
49bUv 138 9,25342
49hNV 163 9,05099
49mwp 160 9,33833
49qO9 154 9,73384
4A2tR 148 8,27337
4ARAB 143 9,04132
4AzuW 154 9,93633
4BKDB 139 9,05183
4BLK0 165 9,46385
4BMCq 142 9,48506
4DvtP 168 9,75589
4F9t5 142 8,52029
4FSIN 141 9,04596
4GABQ 140 9,11282
4GAr9 153 9,29855
4GGBo 175 6,28505
4GdSB 161 7,65118
4GgDf 138 9,41614
4GjdX 139 8,80317
4Gu1B 145 9,32679
4HBfo 152 8,54214
4HtEW 160 7,65442
4IM5q 138 8,81517
4IMEi 142 9,19321
4J2U0 152 9,13673
4KW4w 155 6,08236
4KaIh 159 7,80965
4KkjS 161 9,77955
4NEvQ 140 8,84005
4NHBZ 139 9,01141
4NbFy 161 7,92312
4NpSq 140 6,27618
4OFwc 142 9,09663
4Pyil 154 9,37559
4Rkc4 178 8,47883
4RwJ5 138 9,15898
4Rwcy 155 9,32414
4UnSB 147 5,90650
4UrHs 146 6,05946
4VhRw 140 9,50923
4Vkh3 121 6,70646
4W5UJ 121 9,23511
4Wmhp 143 8,71859
4YFYk 166 8,53827
4ZeEG 140 8,42732
4ZiSc 144 8,92927
4aTwD 139 9,45547
4aWeu 129 8,46304
4awVH 142 9,07942
4bB3m 139 9,22815
4bCRE 139 9,19579
4bEzH 138 9,34948
4bapl 139 9,19579
4bx9y 139 9,03919
4byfQ 152 8,27917
4d1zh 141 9,07961
4dVZk 177 6,31034
4daIX 139 9,51246
4daz8 141 9,15872
4duec 138 9,12313
4eGXF 163 8,65554
4eKVn 157 9,24330
4ePdA 144 9,02456
4eQ4T 138 9,24491
4etUr 150 6,28079
4f6S5 167 6,91432
4fLkf 139 9,28127
4fOiy 140 9,29771
4fUux 140 7,78225
4fm9y 140 5,60980
4fnw3 138 8,79086
4fo2I 138 9,32131
4fpVF 140 7,76997
4frvD 121 9,49015
4gBW3 140 7,76997
4gCQN 153 8,57811
4gDfh 160 8,86042
4gI8i 154 9,62399
4gM39 154 9,27025
4gMuq 139 9,37120
4gPio 163 8,58682
4gQHb 140 6,27573
4gQWd 163 7,85426
4gR87 140 6,49797
4gVu7 160 8,39032
4gWis 161 10,34178
4gZwX 142 9,01128
4ggeR 160 8,28575
4h6BY 142 9,25355
4h9PF 155 9,48306
4hgvV 143 9,02456
4hoWQ 145 10,13013
4jtmf 139 7,60769
4kAa5 163 4,54817
4kKp7 142 5,73542
4kMbB 142 6,04621
4kVol 143 5,37159
4mIkF 142 9,18251
4mKNq 142 9,18251
4mjX4 107 9,30074
4mqQl 198 6,20684
4nv13 160 9,54475
4p6gC 139 9,09779
4pR6m 167 9,00980
4phRn 139 8,94243
4ppse 139 8,75985
4psRt 148 8,99651
4pxCA 142 9,09760
4qHcu 190 8,95403
4qMXl 138 9,09760
4qSBm 126 8,70576
4qqGg 144 9,06247
4r4Dq 147 9,68504
4rMIr 140 10,14792
4rP5f 142 9,36521
4rhn3 139 8,79060
4uIkX 166 9,90752
4w94f 142 9,02475
4wVb4 142 8,93166
4wZLF 138 8,82690
4yIHH 167 5,77418
4yXMf 139 8,49714
4zTyF 156 8,98788
4zp1R 140 7,68738
506kz 140 9,36553
5071T 142 8,69209
5089M 138 9,19527
50GX1 139 9,40009
50kyx 119 8,84314
50vXn 142 9,29984
518HO 139 8,77545
52FvH 138 7,63890
52P60 146 8,60796
52ixX 149 8,94900
53oPj 119 9,51117
53pJN 139 9,01141
54p8r 138 8,59855
54z5M 138 9,27599
555nR 139 9,11694
55KVD 150 9,42871
56GYD 139 8,91316
56GaH 139 8,42745
56O3u 139 9,36540
5796N 142 9,21900
57HHr 154 9,78071
57Je7 142 8,94546
57KtX 138 9,36514
58SVG 152 9,45534
59FoF 138 9,34941
59J6a 138 9,09760
59R8f 139 9,05866
5BIHL 138 8,79131
5BuEm 167 7,68880
5D7ih 145 7,72865
5DAAy 139 9,16362
5Dhke 146 9,26071
5EB4e 249 5,60548
5EH0q 149 9,06666
5HnaV 154 9,30016
5HvuI 142 8,94565
5I9DM 156 9,36463
5Ic3Z 141 9,07633
5JFar 139 9,23163
5Jizf 165 7,06323
5JrsB 149 9,04106
5aADe 139 9,35928
5axXq 154 8,98175
5cIDZ 140 9,20262
5cITi 139 6,27385
5cNgi 159 8,97924
5ct77 138 8,94546
5cy9y 142 9,09792
5cyKz 140 8,96583
5czBb 129 8,73348
5d5Pw 141 8,94243
5dS07 145 9,19069
5dbZn 119 9,15201
5dcR8 139 9,24362
5dt4O 154 9,82603
5duxk 142 8,96306
5eMZe 139 8,65270
5eRNn 139 9,25806
5f7V3 154 9,48847
5fDIL 140 8,94533
5fWkD 119 9,15169
5g2bR 138 9,01102
5gnrd 142 9,13731
5hAqj 174 9,11456
5hJOo 138 9,03049
5hmZH 138 9,17335
5hxZe 140 9,32137
5hyfK 142 8,92921
5ic0j 143 9,18270
5jgbt 154 9,07923
5jmLf 142 8,67946
5kB7Q 154 7,61520
5kMZa 153 8,34603
5kT6f 141 8,93276
5kXHc 142 9,20268
5kZ2L 140 7,73302
5kynr 143 9,07961
5l1jt 158 8,89936
5lFIX 139 8,95281
5lIK4 141 8,92953
5lTgC 142 9,20223
5lUi0 138 9,45476
5mIML 143 9,09760
5mQzM 139 8,70615
5mXSJ 142 9,35451
5mhRt 147 9,54172
5mtpk 142 9,18270
5muQM 143 8,89826
5mupM 138 8,79131
5mw03 139 9,11649
5mxve 143 9,11701
5n6Yu 142 8,20587
5nfqv 119 9,20352
5ng9X 208 5,02146
5nzwQ 142 9,25355
5o4G4 144 9,00896
5o8vY 142 9,27966
5qTUI 139 9,01128
5sQtf 170 5,61145
5tF9H 158 10,23134
5tLpk 142 9,25329
5tn2r 139 9,03107
5tqfd 142 8,94565
5vJbM 153 9,72688
5vKof 138 9,25342
5vddN 142 9,38971
5vm4y 142 9,37630
5wtls 142 9,09792
5xFJA 138 9,25342
5xaBr 142 8,34983
5xzT6 140 8,89788
5y6xF 141 9,21216
5yQML 139 9,26038
5zHcH 119 9,07394
5zKpG 139 6,88936
5zLgD 139 9,32466
63mg 140 8,92895
6A3nP 140 7,77219
6AxVq 139 8,94610
6BUBR 154 9,17284
6BUNF 142 9,32395
6BYTC 151 6,41902
6GVN8 119 9,24401
6GVVL 139 9,21848
6GgfR 156 9,72694
6Gvmq 105 9,16729
6IId7 149 9,18238
6KWD1 119 9,05189
6KWOl 174 9,78838
6Kn2T 165 6,71168
6KxCB 141 8,84005
6Kyia 141 5,29008
6KzeO 138 9,03088
6L74D 139 9,24401
6LEvi 171 7,97179
6LNtI 158 9,19289
6LQHg 159 9,21983
6Lx3N 142 9,41569
6M9ww 139 9,09760
6MAtq 142 9,21880
6Mme3 140 8,86751
6PXuZ 139 6,80302
6QZLB 143 9,19546
6QugC 141 7,69136
6QxKL 140 8,62963
6R0Ri 157 9,43696
6RbXW 157 8,29787
6RsTd 148 8,86706
6SQXS 156 9,35702
6TKL9 139 9,32866
6TZly 139 8,36988
6U4iX 141 9,36605
6UbMh 138 9,36482
6UtML 139 9,07736
6Uwk6 139 9,33878
6VGIE 138 9,15949
6VXqK 143 8,63059
6VYHd 164 5,37642
6Vy2g 154 9,62444
6VygN 140 8,80711
6W2KA 138 8,64432
6WDMA 151 5,28321
6WHGf 178 4,76745
6XyXU 168 6,38173
6wVY1 142 7,70495
6wVpg 130 9,05350
6xNtJ 138 6,71328
6xXrv 138 9,27599
6xZnS 140 5,74627
6xjuv 139 8,84824
6xqZb 149 8,94900
6yZLu 143 8,92902
6zMLo 119 9,09767
6zaxy 142 9,02456
6zhzx 151 9,96934
7BCuT 148 8,90568
7E7MH 142 9,25336
7FkQe 142 9,09773
7G5FZ 154 9,15872
7IAG9 138 9,36547
7Kh3I 155 9,31866
7LbaL 142 9,11688
7Lzk0 121 6,09191
7X2I4 149 8,86958
7XEFx 138 9,13731
7XUoG 139 8,69248
7XWb0 161 9,45791
7XsQ3 138 9,24394
7Xxod 119 7,61463
7Y1hv 142 9,25329
7YVFJ 154 6,71572
7cqHx 148 9,69522
7dvSJ 137 8,35583
7dvTx 167 9,00857
7jiTP 141 7,70927
7k5W1 143 9,23202
7kPM4 189 5,34454
7qSNe 143 7,62952
7vKnJ 143 6,27653
7wBx 142 9,38964
7wg7i 147 6,51110
7wgsb 151 6,90011
7wmtV 159 9,39209
7xGPj 155 7,74434
7y9W 140 9,23202
7ykQT 148 9,58234
805nJ 139 9,19501
82SrF 147 7,68551
82z6s 138 9,07955
83FaL 138 8,69300
83Fbt 138 8,70628
83Wl5 140 6,81871
84pSQ 144 9,07949
87cdN 149 9,16362
87cr9 139 9,02456
87dij 138 8,70550
87fFq 136 9,04570
87lyE 141 8,91354
87qQr 139 9,32459
87rlj 138 9,19579
88aDm 145 9,05131
891KT 139 8,88228
89FtN 139 8,87009
89qL3 138 9,25342
89qwE 142 9,65809
8E4vQ 141 9,41498
8ERL5 138 9,11694
8IpD 138 9,21235
8Kzh 139 9,41530
8aJaE 155 9,00154
8aNsa 139 9,00180
8aPMq 138 8,73348
8aQzo 171 8,25661
8aqnB 162 7,00674
8b5Wg 138 7,62759
8b6Hs 142 8,71917
8bM3i 138 8,31818
8dHQI 140 8,80666
8dHSI 142 9,09741
8dX3Y 161 5,39399
8dYNf 142 8,34622
8dZGZ 142 9,15859
8eKmI 139 8,99226
8fLe4 139 8,39857
8iWea 146 8,65264
8iw1q 160 9,29990
8jC6n 138 9,09792
8jg44 171 8,25435
8likQ 148 7,72586
8m345 139 9,46662
8mC58 159 8,98742
8mDXX 138 9,19527
8mWGO 139 8,92895
8msFH 149 8,82703
8mxw1 140 8,82729
8nm9u 142 9,03068
8nnhX 153 8,67881
8npiN 163 9,21783
8nylW 138 9,21893
8o8ql 143 9,04609
8oZSk 154 9,30003
8odeE 142 8,97956
8p53M 139 9,21893
8rbch 163 9,05099
8rw0g 142 9,41530
8s5cF 138 8,94507
8su3z 138 8,91296
8thXE 139 9,09792
8tifq 142 8,46317
8vLsi 161 9,41981
8wB5o 141 8,35576
8zEQ5 139 8,91264
8zgAP 161 5,21193
BEvB 138 9,11694
Cfph 138 8,94546
DcAq 141 8,73432
Fc7F 142 9,25355
Fmjl 132 9,45469
GQlN 139 9,30106
Gldo 138 9,25342
HdWd 145 8,46136
IIub 139 9,25768
IKLa 139 9,38075
IUcr 138 9,29971
IxI3 152 9,31847
J8RN 138 9,43645
JDlm 138 9,34941
JI17 138 9,15898
JJlN 139 8,85862
JbBj 142 9,04119
JhiT 138 9,05138
JqRF 142 9,42839
Jxso 139 9,12926
Kuj8 139 8,94565
L0XI 139 9,23208
L4nx 139 9,08219
LCiT 139 9,23208
LhyX 143 9,09760
LkUF 158 9,32408
MHjw 138 9,18270
MMaU 138 7,70620
MlpA 138 9,05138
MsbI 139 9,07742
MvJx 139 9,11430
QwMC 142 9,43464
Qwnp 140 9,48499
R8AV 137 6,63353
RJv5 138 8,45047
T9JX 139 8,45034
TGjU 138 9,19585
WdN5 140 8,89865
Wdj9 139 9,28192
WecA 166 9,42542
aBoj 151 9,13602
anyn 139 9,25355
bPXD 138 9,48344
dgiM 154 9,71618
dgta 154 9,67898
eEC3 139 8,69287
eroE 140 9,34890
esg4 140 9,23176
jODP 139 9,19559
jRtw 168 9,54514
kYUH 140 9,29984
klRy 171 5,79686
lK9r 138 9,37617
mkjB 141 9,08200
oti9 138 8,91296
udeT 138 9,03094
x9vx 138 8,97492
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_26883
4fOqa
 24 32,8% 152 3.828E-49
2 phalp2_34744
3emJf
 6 35,2% 156 1.530E-46
3 phalp2_35615
1iltm
 50 32,8% 146 2.098E-46
4 phalp2_32878
3eE2c
 24 33,1% 157 4.576E-41
5 phalp2_20305
2fycX
 1 25,7% 159 8.799E-38
6 phalp2_29493
1NtQH
 15 35,2% 159 1.093E-36
7 phalp2_19828
5klLF
 236 35,0% 157 4.404E-31
8 phalp2_21422
84qjx
 127 27,5% 167 2.628E-29
9 phalp2_19861
5zIVL
 30 26,5% 173 6.095E-28
10 phalp2_39364
4EoD5
 60 26,7% 153 1.565E-27

Domains

Domains
PET_M15
Representative sequence (used for alignment): 4SR8r (162 AA)
Member sequence: 5mlgT (139 AA)
1 162 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF13539

Taxonomy

  Name Taxonomy ID Lineage
Phage Unknown from Metagenome
[NCBI] 		UNKNOWN_ENVHOG No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)

No CDS data available.

Gene Ontology

No Gene Ontology terms available.

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4SR8r) rather than this protein.
PDB ID
4SR8r
Method AlphaFoldv2
Resolution 94.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50