Protein

Protein accession
2XS7d [EnVhog]
Representative
1iBPp
Source
EnVhog (cluster: phalp2_29414)
Protein name
2XS7d
Lysin probability
99%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGKWVMPFPQSKITGHYGTLSAYRKKMGMQAHSGTDWAMPSKTLIPAITDGTIKLIQFSKILGWVVVQTGWDVKAKKTKYIGYCHLWCDKHGAKCGGPTAGCKSPSTSFKLGQKVKVGEKFGIRVSTTGSASTGNHLHATLGNSVKSVFGTTAQKEDLYRFIQSQSKGAAPKQANPQTKAVVESGTKTQVAPATKIVYACPHCKKELN
Physico‐chemical
properties
protein length:208 AA
molecular weight:22433,8 Da
isoelectric point:9,90
hydropathy:-0,36
Representative Protein Details
Accession
1iBPp
Protein name
1iBPp
Sequence length
183 AA
Molecular weight
19810,64690 Da
Isoelectric point
10,03259
Sequence
MTWKLPFPDKYVTGEFGTRSKFRLANNLGPHRGTDWSKPEGTKIPAITSGTVVLVQYSKMLGWCLVHTGWAENKTWHIGYSHLMEEPKLKVGDKVKVGQTIGLMGSTGTASSGPHLHATLGTGPKSIFIGKVYDLKAFIKKQATESSEKPQKPTPKEKTPSKGSKPSKPTTAAPRPKSPKSGG
Other Proteins in cluster: phalp2_29414
Total (incl. this protein): 693 Avg length: 160,3 Avg pI: 9,46
Protein ID Length (AA) pI
1iBPp 183 10,03259
12IBZ 152 8,99149
12MIr 146 8,13083
12Ncn 148 8,80820
14d6n 174 9,65203
14nF6 155 9,12726
16n9N 148 9,79863
17w3v 226 9,52722
182me 148 9,24743
18Eup 143 7,77116
18F0M 153 8,80775
18Jy3 177 9,51968
18jSf 148 8,75650
18mxS 153 9,16297
19JNp 152 8,65270
19MZf 152 8,65174
19QIN 154 9,27328
1AGdG 180 8,83999
1Czcg 176 9,71998
1DB77 175 9,81236
1I8mn 142 9,97572
1JCnR 165 9,74867
1JEUH 149 9,43277
1JGGd 153 9,25574
1JMb1 236 9,94181
1JN1c 162 9,62934
1JORl 162 9,64893
1JQKb 148 9,64075
1JQL7 151 9,54314
1JRW0 159 9,73229
1JVNy 163 9,47268
1JVmU 163 9,58543
1JXLO 162 9,59298
1JZAO 163 9,58550
1Jp1c 153 9,39719
1JvjN 155 9,54682
1Jx6W 153 9,44347
1K2Eb 176 9,13822
1K52t 162 9,68465
1K7qt 178 9,29874
1KSI3 159 9,69503
1KSeH 166 9,94562
1KSiy 171 9,55707
1KZ0i 152 9,61161
1KaCN 147 9,45978
1Kd50 163 9,47062
1Ke11 176 9,63733
1KgYQ 147 9,45985
1Kgh9 160 9,59298
1KrGE 163 9,66989
1LAEh 142 8,57225
1LAbb 169 9,54450
1LOv6 167 9,87109
1LP2O 163 9,58543
1LUY2 160 9,59755
1LV4I 152 8,86790
1LXku 160 9,79031
1Lgzg 153 9,22241
1Ll1v 144 10,00216
1LtwB 154 9,62644
1Lv4E 153 9,49408
1LyVS 153 9,66763
1M1i0 164 9,60548
1M3fx 161 9,51710
1MAlN 162 9,81816
1MCdB 163 9,58550
1MHbR 170 9,66035
1MMm9 162 9,56738
1MdyF 156 9,22763
1MiDe 174 9,67627
1MiwU 174 9,60922
1Mkow 159 9,85652
1MnxM 161 9,59227
1Mv3C 169 9,56674
1Mx7R 159 9,30119
1dZZI 177 9,67143
1eBmh 174 9,55365
1ex62 153 9,39751
1kx2c 138 9,39731
1kxSB 148 9,22273
1mN90 177 9,63617
1oZgx 152 8,78248
1pGq6 148 9,46894
1q99z 144 9,93685
1wTFE 160 9,54765
1zIgw 205 9,53199
21dtQ 159 9,27785
24PTY 146 8,00035
24Qmp 148 9,24749
24Rm7 154 9,24169
25LHJ 155 8,78177
25MaN 154 9,21448
25Obk 153 9,12726
25Oeb 138 9,69838
25OgL 146 9,24743
25Ov5 152 8,69300
25fGC 148 9,07336
25sWh 170 10,05044
26Tdh 198 9,59375
26m9y 206 9,58692
27RLT 154 9,75073
27RhC 152 9,51948
27Tec 152 9,51948
27VCl 152 9,39719
298Nh 199 9,72804
2ERzG 140 9,67685
2FOIq 142 10,02717
2FbuY 142 9,83028
2G0EO 170 9,42755
2GbKb 186 9,67859
2Gd38 166 9,92989
2Gy0I 148 9,32917
2I9Bl 165 9,38758
2Ikl3 300 10,02846
2Jgjf 166 9,97005
2JllR 195 9,80991
2MEAg 174 9,84369
2NJE 138 9,92434
2Pjn 140 9,93891
2RVCQ 154 9,24710
2T6Nf 226 9,54733
2T9Mf 226 9,66789
2TamR 227 9,26586
2Tc0r 200 8,82361
2X0Fl 163 9,58543
2XSPB 195 9,80959
2cPOb 147 9,30113
2cWuJ 153 9,12655
2cYO5 153 8,78177
2cYn7 149 8,63091
2cj7G 209 9,40466
2cvtR 182 9,67259
2d2rV 152 8,71350
2d32b 165 9,74867
2d3Mw 154 9,15588
2dAeP 140 9,75015
2dDkH 170 9,50562
2dGED 140 9,67627
2emAm 173 9,87490
2g12w 140 9,61470
2gmzB 237 9,39435
2hACB 153 8,78113
2hDBP 152 8,78248
2hF3f 151 9,39377
2hJ4u 180 8,51010
2hS1T 153 9,04835
2hWPA 172 9,58285
2hzUZ 148 9,24749
2itzN 146 9,04861
2k51J 140 9,71676
2koD7 142 9,87187
2kqYS 142 9,87264
2lnKt 137 9,81649
2lptn 140 9,93891
2q8FK 140 9,91680
2qjFl 285 10,01570
307xS 140 9,44399
30B3A 152 8,65000
30BFg 154 8,01331
30COa 146 9,07317
30OET 177 9,71198
30PaT 154 9,12674
30dMJ 153 8,78183
30iVq 153 9,12674
30k7q 142 8,61396
30kxy 152 8,96879
30l3M 150 9,39377
30lAz 142 8,49637
30xPr 155 8,65290
31AAp 140 9,30074
31OPA 152 9,48867
31XFI 143 7,00384
31uy6 140 9,07381
32xz7 154 9,12706
33qYU 140 9,88321
34Jj1 191 9,74880
34K8N 140 10,11923
34p1i 140 10,06392
35aks 170 9,50614
36LX1 154 9,24717
36XS0 178 9,32234
37ebQ 178 9,54682
38bql 153 9,49408
38ddA 157 9,51619
38fp6 177 9,53985
38ikw 178 9,29874
38is6 155 9,46784
38kay 178 9,37933
38nMr 177 8,01292
38nvB 177 9,38365
38q7y 156 9,55172
39P7n 140 9,89772
39RDO 170 9,50562
39Tom 120 10,00486
39Wfb 140 9,89856
3NXZn 140 9,55178
3NYcC 140 9,75015
3NZ5a 140 9,85414
3NihO 140 10,36009
3NlK1 172 9,72269
3NsUF 140 9,67685
3O2tY 168 9,97663
3OfpS 140 9,75054
3OlCk 140 9,75015
3RHQq 140 9,85407
3So6I 140 9,79070
3SoT 174 9,20610
3Tiv 138 9,92434
3U2hL 154 9,33053
3Uf4A 179 9,72249
3Ur0o 140 10,00048
3WpEP 140 9,88321
3WvTn 140 10,00048
3XtiR 144 9,32995
3YLLL 163 9,61354
3aIlr 194 10,56716
3aUQn 181 9,77026
3aUoN 191 9,96238
3b0ST 153 8,83521
3b2je 151 8,80859
3b4Vi 146 7,97617
3b4y6 153 8,78248
3b5Ft 152 9,12674
3b7aE 153 8,83521
3bMpM 195 10,17906
3bhEJ 152 8,00099
3biRe 153 8,96847
3bkPa 158 8,55175
3bp7M 131 9,36237
3bvQ6 140 9,69645
3bwKY 140 9,83505
40nIJ 153 9,25574
43BDO 161 9,81720
43K31 163 9,61354
43VJC 140 10,00048
43sDq 168 9,17799
43uAP 153 9,49408
43woh 149 9,52161
444ZF 139 9,27276
44dUR 142 9,39777
463rs 153 8,63195
466h3 176 9,79386
466q7 139 10,10105
468lp 154 8,65193
46AzW 156 9,07330
46Mdf 153 9,09870
46dSu 155 9,07259
46hdG 163 9,72546
46iuC 146 9,68439
46iyZ 143 9,21216
46jwM 151 9,14028
46tR1 152 8,99149
46wPa 152 7,94755
46zGl 153 8,78106
477m2 152 8,99200
47CRX 152 8,67011
47Dki 151 9,04873
47IBv 153 8,80865
47JYn 140 9,06988
47MW4 143 9,62682
47fVW 145 8,75798
47uBT 148 9,07336
47w96 177 9,61986
47y5F 154 9,12706
485SC 152 8,67275
486Nb 154 9,02507
487Fm 146 9,24743
48AaD 156 9,27315
48IXm 140 9,27141
48WqB 152 8,78248
48XLs 148 9,07336
48Yo1 155 8,80794
48cU5 125 8,92895
48e5i 152 8,65374
495Q5 165 9,74867
496Oq 163 9,69800
49Fcw 153 9,45443
49MTI 142 10,33410
49OfH 189 9,47184
49Q2y 146 9,83383
49U8Q 175 9,41098
49UsR 165 9,74867
49VYB 153 9,25574
49oKc 178 9,42607
49pPD 172 9,49879
49rgG 169 9,54450
49tvZ 110 7,01782
49x5l 163 9,72546
49yIq 177 9,49395
49z1H 161 9,62025
4A0Ex 146 9,73958
4A0Hz 198 9,58782
4A5D4 147 9,51793
4A7vb 140 10,23766
4ARVZ 149 9,66002
4AbsA 151 9,79302
4Aeb2 200 9,57512
4AfKv 135 9,40499
4AfNh 152 9,77832
4CHIt 168 9,72391
4CHJf 140 9,63978
4CHSO 170 9,57576
4CHTj 140 9,73455
4CQlw 140 9,67627
4CQne 151 9,45037
4CQy5 140 9,86349
4CRGD 140 9,67685
4CRMj 170 9,42671
4CRY0 140 9,62818
4CRoP 140 9,67627
4EOn 199 9,98649
4J0wf 154 7,14048
4Jakd 132 9,94542
4Je8E 146 7,97617
4KJEu 140 9,67627
4KJUM 140 9,67627
4KJUo 140 9,67633
4KJeT 170 9,50543
4KJrh 140 9,89746
4KLZP 171 9,08471
4KM0O 140 9,75015
4KNie 140 9,44264
4KPon 140 9,67633
4KSps 140 10,19530
4KTn4 174 9,60806
4KUaK 140 9,44264
4KUj3 142 9,92899
4KkDu 140 9,93891
4KnY8 142 9,69625
4NCbc 165 9,58550
4NHWj 181 9,61335
4NIgC 167 9,79818
4NOuz 145 9,89578
4Ny2Y 157 9,35193
4PyjK 228 10,08680
4Rq6o 140 9,63978
4RqAI 140 9,58788
4RqIY 140 9,79070
4Rqlw 184 9,20288
4SSf7 206 9,57035
4VekE 228 10,06140
4W7ZO 140 9,97631
4WZ9n 140 9,97553
4XD6S 222 10,22464
4XEBY 140 9,67685
4XPLE 159 9,83957
4XPN5 159 9,83957
4YnIr 182 9,81797
4Zdls 140 9,88231
4a0WG 178 9,32208
4aE7V 161 9,46868
4aEZa 152 8,99149
4aH9g 163 9,69800
4aKex 154 9,07291
4aKq4 142 8,94752
4ab4n 153 9,33020
4adBu 149 9,61980
4ahbj 153 8,96847
4ai8g 155 9,42536
4aiMY 176 9,59265
4ainm 151 9,12739
4ajOX 153 9,07291
4alpL 150 9,39377
4avZb 151 9,67350
4awXD 161 9,84834
4axvY 146 9,91815
4b829 163 9,64455
4b8lV 143 9,27308
4b9nz 167 9,02572
4bCxJ 150 9,25071
4bGh7 146 9,09908
4bHiY 155 8,67295
4bIF2 143 7,76088
4bIsW 128 9,48074
4bL4M 152 8,99149
4bLpC 140 9,55378
4bY8u 176 9,49428
4bg9n 140 9,93795
4bhH8 140 9,83383
4bhYm 145 10,00042
4bqtd 179 9,79386
4bwrP 153 9,57596
4bxyL 150 9,27508
4bysb 150 9,22815
4c1um 157 9,51619
4cgtv 171 9,19727
4cuxq 147 9,41614
4egsd 166 9,60903
4l4K5 159 9,30126
4l6Ga 165 9,74867
4lhpn 171 9,76762
4lkTs 177 9,67027
4lqWa 131 9,25987
4nO7m 203 9,54920
4nQh1 210 9,40956
4nRnU 208 9,99577
4nSre 207 9,51413
4nfe5 170 9,14408
4oTgn 142 10,33410
4oVKF 140 10,19124
4oaqu 167 9,66911
4ocEa 149 9,79302
4oegM 152 9,63675
4ogbM 146 9,64977
4ohGa 136 9,76820
4pBAt 178 9,40196
4pWsV 149 9,79302
4qJ7K 140 9,93795
4rPny 146 9,32698
4rb4l 146 9,32575
4rey9 154 9,09863
4rjDh 152 8,04889
4rjP0 171 9,81668
4sa7Z 154 9,49402
4wkpy 170 10,17932
4xUsa 168 9,69638
4zP4w 194 9,49621
4zR2g 152 9,84053
4zS37 167 9,56158
4zVaH 184 9,69890
50Fri 177 9,67053
50NL0 162 9,68317
50OVj 163 9,64455
50Oqh 162 9,59298
50a8q 146 9,90926
51BfC 182 9,81797
51lmi 182 9,66402
52Ey8 178 9,53773
533G7 187 9,73687
533L1 166 9,43277
54eDD 177 9,64500
557rI 147 9,46056
558ov 182 9,81797
55GHE 160 9,51968
56pNC 163 9,56738
56rRF 165 9,79431
580yz 154 9,34664
5Axdn 170 10,17932
5Axoy 142 10,22735
5BWm2 136 9,83273
5CbYq 142 9,88231
5CeFh 142 10,13825
5CeKg 140 10,00048
5D1qU 146 9,65686
5abm6 178 9,40170
5adNa 146 9,45978
5bssZ 140 9,88321
5bsxj 140 10,06250
5buGX 140 9,88321
5c1Pj 140 10,00048
5cD2p 155 9,55178
5cOkM 163 9,61354
5d0Xd 155 9,55094
5dAXS 135 9,79747
5diZc 136 9,90732
5diu0 165 9,74867
5dwRK 144 9,32995
5dy1Z 163 9,69761
5eJLf 142 9,01469
5eSQC 162 9,13035
5eVah 142 9,21132
5esbT 142 9,16233
5euAY 158 9,59259
5fhn7 189 9,49640
5gphR 179 9,79386
5hjH3 140 10,00048
5kTDF 160 8,95919
5kUY2 146 8,65006
5kqYV 165 9,77652
5krYV 181 9,33807
5l9Yz 148 9,57061
5lAUJ 148 9,69535
5lBXx 148 10,00396
5lOoE 179 9,72249
5lRcl 182 9,66402
5lib8 148 9,67562
5lnxZ 119 8,06024
5lw1W 140 9,93795
5ma8c 158 9,68072
5maJS 163 9,56738
5maLX 161 9,47268
5madl 176 9,16639
5mahi 146 9,65686
5mavO 147 9,45978
5mbKj 163 9,56738
5mbl4 161 9,51710
5sRCd 158 9,59298
5sWcX 198 9,81636
5tepf 222 10,27828
5uJvU 146 9,95851
5uOnf 156 9,54540
5wScP 148 9,44206
5wdeW 146 9,32698
5x2La 182 9,93543
5xtTW 157 9,62637
5yPAr 140 10,21581
5ypos 187 9,73687
5yvvQ 171 9,68310
5ywKE 144 9,67201
5zF2G 148 9,63926
5zIY7 163 9,56222
5zMtV 185 9,76047
5zOcJ 159 9,62063
6ADqx 140 9,83460
6AFB5 177 9,34767
6BNFZ 149 9,52193
6BOWw 160 9,76807
6BR7Q 153 9,34664
6BSsf 155 9,55094
6BVV0 159 9,40827
6BVfj 149 9,11888
6GAnB 171 9,60890
6GLR6 147 8,59668
6GUh3 146 9,07317
6GjG4 151 9,66615
6Gk4e 171 9,62940
6GkDD 146 9,32704
6GlbH 179 9,72855
6Gm9l 157 9,35199
6Gmg7 163 9,58550
6Gn8K 148 9,57834
6Go3X 187 9,57841
6Go4a 163 9,51774
6Gz3o 160 9,76807
6IHEt 142 9,55114
6II5W 163 9,56738
6IIgX 156 9,27785
6IJgv 163 9,65499
6It29 163 9,58543
6ItSK 165 9,25181
6Ixfl 140 9,58859
6KSqP 178 9,84531
6Kwrj 129 9,56990
6L8dh 146 9,71334
6LDLH 146 9,39783
6LzyO 146 9,39783
6M334 176 9,30068
6M4JN 148 9,54649
6M74z 147 9,45978
6M7AM 135 9,91042
6M7aJ 176 9,32176
6M8AA 162 9,70999
6M90g 163 9,56738
6M9pD 161 9,62747
6MG32 140 9,75963
6MeCx 145 9,94491
6MeL5 145 9,93685
6MfHL 142 10,22735
6Mjnk 163 9,65499
6Mjo2 151 9,56957
6MoHq 148 7,89475
6Mt87 154 9,09895
6MtPB 156 9,45476
6QhYs 150 9,32924
6Qi7G 163 9,72823
6QiP9 236 9,94181
6UoBv 140 9,86245
6Uoe2 140 10,00048
6VKBF 151 9,05228
6Vz0N 159 9,83957
6xGG7 146 9,32698
6xvoo 140 10,00048
7LgYt 149 9,52161
7LsqK 168 9,44135
7W6Tn 146 9,48847
7XFgy 162 9,25052
7XKde 142 9,18618
7XLJc 157 9,18766
7XOu3 157 9,01592
7XZsQ 153 9,25568
7Xd96 178 9,13287
7XghW 153 9,07278
7XqJX 198 9,69825
7YjrS 179 9,24259
7ZBCh 177 9,47287
807m6 167 9,54243
80RKF 140 9,51806
80c1l 152 8,99149
80nFc 192 9,61199
83FUK 146 9,07317
83nHH 143 9,32762
84Gxk 146 8,00054
85FAI 163 9,56738
85FG2 178 9,75041
85NY9 191 9,92389
87mCp 179 7,87689
88fYF 152 8,96873
88gKQ 140 9,18592
89rG8 144 8,76146
89sbi 142 7,90455
8APpZ 227 9,97875
8BdRn 167 9,42246
8BiwD 196 9,68704
8CuOK 195 9,17458
8DcOL 140 9,94497
8DhSi 173 9,31576
8DhsG 193 9,52851
8E3N5 196 9,57989
8E5qu 196 9,66776
8ERPC 187 9,67827
8EZqn 168 9,69638
8FdPs 166 9,48744
8Flzi 193 9,68891
8GGzW 170 9,57286
8b6rN 176 9,09870
8eLG 151 9,48203
8eN9V 165 9,77652
8lv7B 161 9,84350
8m2LK 153 9,46784
8m2pE 163 9,35199
8mS8H 153 8,83515
8mTtL 152 8,99149
8mtcX 140 9,18592
8nApC 154 9,12706
8nlr3 142 8,96802
8p5If 146 9,81352
8p5b4 146 9,55262
8p5mF 145 9,83312
8pxj7 153 9,81288
8r9U7 143 8,43209
8rC6l 153 9,52155
8rQdd 219 8,40876
8rRzU 204 5,47458
8rclD 144 9,52077
8s3Nx 194 9,05950
8s3k5 200 8,95712
8s3pc 227 9,08935
8s4vp 182 9,71727
8sO2T 155 9,42536
8wEiV 168 9,63881
8wHqS 184 9,76388
8wJHn 140 9,58685
8wJKF 166 9,65596
8xlR4 166 9,60903
8yle8 168 9,63881
8zK1H 204 9,40595
AfH0 205 9,93266
BhKK 193 9,58356
Bhwa 227 9,14969
DFC 183 9,62624
FNjg 165 9,68497
FOAi 199 8,59249
GXuv 153 9,24710
GYUR 163 9,72546
H04v 139 9,24665
H7nj 140 10,00048
HejJ 169 9,54450
IQsH 152 9,62998
J08b 151 9,27296
Junc 163 9,52038
KeLV 147 9,62063
LEHh 146 9,91815
LNyy 154 9,19907
LUS5 134 9,73448
RYdR 152 9,01592
RcZD 182 9,67363
SEVd 140 9,55378
SOnp 154 9,07291
SpeV 153 9,12655
SsbU 152 8,99149
TCh3 153 9,09915
TO7N 140 9,44399
TYJS 146 8,78203
Uii0 168 9,19920
Uk73 176 9,68272
UuNU 149 9,46791
X8KB 197 10,12619
X9fs 197 10,15288
anKL 140 9,75963
clyO 166 9,90578
cpZn 206 9,17696
cqz9 186 9,53012
crAJ 185 10,07230
crAX 184 9,55352
crof 206 9,06214
dW0N 155 9,12706
e4qq 155 8,65193
eL3j 164 9,60471
fS8c 197 10,06263
gGni 235 9,42097
iWNT 161 9,46062
ilCn 198 9,72855
irTV 185 9,46533
itJT 197 10,06263
jVnn 155 9,27328
jWtA 140 10,00048
koqA 140 9,48183
vfeC 178 9,71927
yGqs 174 9,86046
yYmN 181 9,52877
yy10 140 9,89914
A0A6J5KPV0 155 9,65068
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_26528
804oT
 5 41,0% 190 2.198E-47
2 phalp2_24155
2cPfi
 141 43,7% 151 1.453E-46
3 phalp2_40030
25nI3
 8 30,2% 185 7.014E-46
4 phalp2_4831
5ksbg
 93 42,4% 146 2.050E-40
5 phalp2_8002
5ma4R
 98 45,3% 128 5.266E-40
6 phalp2_32978
49AHS
 6 27,2% 154 7.035E-25
7 phalp2_40405
499XR
 8 25,9% 158 1.125E-20
8 phalp2_1234
l7WO
 2 29,7% 148 1.536E-20
9 phalp2_40658
5cY9d
 663 34,5% 136 3.458E-19
10 phalp2_39871
17EO3
 18 31,2% 147 4.720E-19

Domains

Domains
PET_M23
Disordered region
Representative sequence (used for alignment): 1iBPp (183 AA)
Member sequence: 2XS7d (208 AA)
1 183 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01551

Taxonomy

  Name Taxonomy ID Lineage
Phage Unknown from Metagenome
[NCBI] 		UNKNOWN_ENVHOG No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)

No CDS data available.

Gene Ontology

No Gene Ontology terms available.

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (1iBPp) rather than this protein.
PDB ID
1iBPp
Method AlphaFoldv2
Resolution 88.14
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50